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mmu_circSnrnp70_312
Basic information of circular RNA
PanCircBase ID:
mmu_circSnrnp70_312
mm39_Chr. Position ID:
chr7|45030140|45036877|-
Gene name:
Snrnp70
Transcript:
ENSMUST00000074575.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,3087,6526,6672
Genomic length:
6737
Exon/intron sizes in nt:
102,82,63,65
Flanking exon/introns:
chr7:45026867-45030140|chr7:45036877-45041502
Splice length:
312
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGGACCCTCACAATGATCCCAATGCTCAGGGCGATGCCTTCAAGACTCTGTTCGTGGCTAGAGTGAACTATGACACAACAGAATCTAAGCTTCGGAGAGAGTTTGAGGTGTATGGACCCATCAAAAGAATCCACATGGTATACAGTAAACGCTCTGGAAAACCCCGTGGTTATGCCTTCATCGAGTATGAGCATGAACGAGACATGCACTCCGCTTACAAGCACGCAGATGGCAAGAAGATAGATGGCAGGAGGGTCCTCGTGGATGTGGAGAGGGGCCGGACTGTGAAGGGCTGGAGGCCCCGGCGCCTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTACAAGCACGCAGATGGC
59.9
CAGAGTCTTGAAGGCATCGC
58.99
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTACAAGCACGCAGATGGC
59.9
AGCCACGAACAGAGTCTTGA
58.96
158
PCR template sequence:
GCTTACAAGCACGCAGATGGCAAGAAGATAGATGGCAGGAGGGTCCTCGTGGATGTGGAGAGGGGCCGGACTGTGAAGGGCTGGAGGCCCCGGCGCCTAGGGGACCCTCACAATGATCCCAATGCTCAGGGCGATGCCTTCAAGACTCTGTTCGTGGCTAGAGTGAACTATGACACAACAGAATCTAAGCTTCGGAGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-27a-3p(1), mmu-miR-324-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCCGGCGCCTAGGGGACCCTCACAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSNRNP70_312_ORF_1
0.9759
0.422962908
0.859989307
489
ORF sequence:
ATGGTATACAGTAAACGCTCTGGAAAACCCCGTGGTTATGCCTTCATCGAGTATGAGCATGAACGAGACATGCACTCCGCTTACAAGCACGCAGATGGCAAGAAGATAGATGGCAGGAGGGTCCTCGTGGATGTGGAGAGGGGCCGGACTGTGAAGGGCTGGAGGCCCCGGCGCCTAGGGGACCCTCACAATGATCCCAATGCTCAGGGCGATGCCTTCAAGACTCTGTTCGTGGCTAGAGTGAACTATGACACAACAGAATCTAAGCTTCGGAGAGAGTTTGAGGTGTATGGACCCATCAAAAGAATCCACATGGTATACAGTAAACGCTCTGGAAAACCCCGTGGTTATGCCTTCATCGAGTATGAGCATGAACGAGACATGCACTCCGCTTACAAGCACGCAGATGGCAAGAAGATAGATGGCAGGAGGGTCCTCGTGGATGTGGAGAGGGGCCGGACTGTGAAGGGCTGGAGGCCCCGGCGCCTA
Peptide sequence:
MVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRL
IRESfinder result
IRESfinder score
0.133543253
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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