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mmu_circSlc26a8_618
Basic information of circular RNA
PanCircBase ID:
mmu_circSlc26a8_618
mm39_Chr. Position ID:
chr17|28861207|28863900|-
Gene name:
Slc26a8
Transcript:
ENSMUST00000114764.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
19,18,17
Exon/intron offsets:
0,282,2315
Genomic length:
2693
Exon/intron sizes in nt:
1,85,55,378
Flanking exon/introns:
chr17:28857666-28861207|chr17:28863900-28866870
Splice length:
618
Other database IDs:
circBase: hsa_circ_0131660, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_122987
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTCTACACAGAGCGATATGAAAACAGACAGGAGCAGGACTCATCCATCAACCTGATCCGCTGCTCCTACCTGGGCAGCGGGGACAGCAGCCAAGTCACTTCTGAGGAACAGATTCCCTACACAGTGTCTTCCACATCACAGAGAAATATAGTGCAGAGCTACGAGGACACAGAGAAAGCCTGGCTACCCAACAGCCCCCCAAGAAACAGTCCACTGCCACCGCCCGAGGCCTCAGAAAGCTTAGCACAGAGCCGATCCAGATCCATTATCATGCCTTACTCGGATACGAGTGTGCAGAACAACACCCATACCATCATTCTGGACTTTTCCATGGTGCACTATGTGGATAATCGGGCTTTGGTTATACTAAGGCAGATGTGCAATGCTTTCTACAATGCCAACATTTTGGTCCTCATTTCAGGGTGTCACACCTCTGTGGTCAAGTCGTTTGAGAAGAATGATTTCTTTGATGAAGGCATCACGAAGGCCCAGCTCTTCCTCAGCCTCCATGACGCCGTGCTGTTTGCCTTGTCCAGGAAGTTCTCTGAGCCTTCAGACTTAAGTATGGATGAGACCGAGACAGTGATACAGGAAACCTACTCAGAGTCAGACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGCTGTTTGCCTTGTCCAG
59.97
CAGCGGATCAGGTTGATGGA
59.82
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGCTGTTTGCCTTGTCCAG
59.97
TGAGTCCTGCTCCTGTCTGT
60.18
144
PCR template sequence:
CGTGCTGTTTGCCTTGTCCAGGAAGTTCTCTGAGCCTTCAGACTTAAGTATGGATGAGACCGAGACAGTGATACAGGAAACCTACTCAGAGTCAGACAAGGTTGTCTACACAGAGCGATATGAAAACAGACAGGAGCAGGACTCATCCATCAACCTGATCCGCTGCTCCTACCTGGGCAGCGGGGACAGCAGCCAAGTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-miR-7b-5p(1), mmu-miR-92a-3p(1), mmu-miR-205-5p(1), mmu-miR-33-5p(1), mmu-miR-7a-5p(1), mmu-miR-203-3p(1), mmu-miR-25-3p(1), mmu-miR-503-5p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGAGTCAGACAAGGTTGTCTACACAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSLC26A8_618_ORF_1
1.1511
0.335703428
0.99446166
963
ORF sequence:
ATGCCTTACTCGGATACGAGTGTGCAGAACAACACCCATACCATCATTCTGGACTTTTCCATGGTGCACTATGTGGATAATCGGGCTTTGGTTATACTAAGGCAGATGTGCAATGCTTTCTACAATGCCAACATTTTGGTCCTCATTTCAGGGTGTCACACCTCTGTGGTCAAGTCGTTTGAGAAGAATGATTTCTTTGATGAAGGCATCACGAAGGCCCAGCTCTTCCTCAGCCTCCATGACGCCGTGCTGTTTGCCTTGTCCAGGAAGTTCTCTGAGCCTTCAGACTTAAGTATGGATGAGACCGAGACAGTGATACAGGAAACCTACTCAGAGTCAGACAAGGTTGTCTACACAGAGCGATATGAAAACAGACAGGAGCAGGACTCATCCATCAACCTGATCCGCTGCTCCTACCTGGGCAGCGGGGACAGCAGCCAAGTCACTTCTGAGGAACAGATTCCCTACACAGTGTCTTCCACATCACAGAGAAATATAGTGCAGAGCTACGAGGACACAGAGAAAGCCTGGCTACCCAACAGCCCCCCAAGAAACAGTCCACTGCCACCGCCCGAGGCCTCAGAAAGCTTAGCACAGAGCCGATCCAGATCCATTATCATGCCTTACTCGGATACGAGTGTGCAGAACAACACCCATACCATCATTCTGGACTTTTCCATGGTGCACTATGTGGATAATCGGGCTTTGGTTATACTAAGGCAGATGTGCAATGCTTTCTACAATGCCAACATTTTGGTCCTCATTTCAGGGTGTCACACCTCTGTGGTCAAGTCGTTTGAGAAGAATGATTTCTTTGATGAAGGCATCACGAAGGCCCAGCTCTTCCTCAGCCTCCATGACGCCGTGCTGTTTGCCTTGTCCAGGAAGTTCTCTGAGCCTTCAGACTTAAGTATGGATGAGACCGAGACAGTGATACAGGAAACCTACTCAGAGTCAGACAAG
Peptide sequence:
MPYSDTSVQNNTHTIILDFSMVHYVDNRALVILRQMCNAFYNANILVLISGCHTSVVKSFEKNDFFDEGITKAQLFLSLHDAVLFALSRKFSEPSDLSMDETETVIQETYSESDKVVYTERYENRQEQDSSINLIRCSYLGSGDSSQVTSEEQIPYTVSSTSQRNIVQSYEDTEKAWLPNSPPRNSPLPPPEASESLAQSRSRSIIMPYSDTSVQNNTHTIILDFSMVHYVDNRALVILRQMCNAFYNANILVLISGCHTSVVKSFEKNDFFDEGITKAQLFLSLHDAVLFALSRKFSEPSDLSMDETETVIQETYSESDK
IRESfinder result
IRESfinder score
0.566654538
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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