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mmu_circSlc25a14_385
Basic information of circular RNA
PanCircBase ID:
mmu_circSlc25a14_385
mm39_Chr. Position ID:
chrX|47717012|47723728|+
Gene name:
Slc25a14
Transcript:
ENSMUST00000134757.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,1116,2753,6621
Genomic length:
6716
Exon/intron sizes in nt:
48,94,148,95
Flanking exon/introns:
chrX:47714517-47717012|chrX:47723728-47726266
Splice length:
385
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAAATGGAGGCAGAGAAAGATTGAATTACTTTCCCAGAGCCACGACTACATCAGAAAAGTTCCACTTTAAGCCATGAGATGTCTGGTCTGAACTGGAAACCTTTTGTGTATGGCGGCCTTGCCTCTATTGTTGCCGAGTTCGGCACTTTCCCTGTGGATCTTACTAAAACACGGCTGCAAGTCCAAGGCCAGAGTATCGATGTTCGTTTCAAAGAAATAAAATATAGAGGGATGTTTCATGCCTTGTTCCGAATCTATAAAGAAGAAGGGATCTTGGCTCTGTATTCAGGAATTGCCCCTGCGTTACTAAGACAGGCATCATATGGCACCATCAAAATTGGTATTTATCAAAGCTTGAAGCGATTATTTGTAGAACGTTTGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCCTGCGTTACTAAGACA
59.75
GATGTAGTCGTGGCTCTGGG
59.9
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCCTGCGTTACTAAGAC
59.75
GATGTAGTCGTGGCTCTGGG
59.9
144
PCR template sequence:
TCAGGAATTGCCCCTGCGTTACTAAGACAGGCATCATATGGCACCATCAAAATTGGTATTTATCAAAGCTTGAAGCGATTATTTGTAGAACGTTTGGAAGAAAATGGAGGCAGAGAAAGATTGAATTACTTTCCCAGAGCCACGACTACATCAGAAAAGTTCCACTTTAAGCCATGAGATGTCTGGTCTGAACTGGAAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-106b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAACGTTTGGAAGAAAATGGAGGCAG
siRNA sets
CTCCATTTTCTTCCAAACG, CTGCCTCCATTTTCTTCCA, CATTTTCTTCCAAACGTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSLC25A14_385_ORF_1
0.5447
0.024515167
0.20150745
384
ORF sequence:
ATGTCTGGTCTGAACTGGAAACCTTTTGTGTATGGCGGCCTTGCCTCTATTGTTGCCGAGTTCGGCACTTTCCCTGTGGATCTTACTAAAACACGGCTGCAAGTCCAAGGCCAGAGTATCGATGTTCGTTTCAAAGAAATAAAATATAGAGGGATGTTTCATGCCTTGTTCCGAATCTATAAAGAAGAAGGGATCTTGGCTCTGTATTCAGGAATTGCCCCTGCGTTACTAAGACAGGCATCATATGGCACCATCAAAATTGGTATTTATCAAAGCTTGAAGCGATTATTTGTAGAACGTTTGGAAGAAAATGGAGGCAGAGAAAGATTGAATTACTTTCCCAGAGCCACGACTACATCAGAAAAGTTCCACTTTAAGCCATGA
Peptide sequence:
MSGLNWKPFVYGGLASIVAEFGTFPVDLTKTRLQVQGQSIDVRFKEIKYRGMFHALFRIYKEEGILALYSGIAPALLRQASYGTIKIGIYQSLKRLFVERLEENGGRERLNYFPRATTTSEKFHFKP
IRESfinder result
IRESfinder score
0.554205454
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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