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mmu_circSeptin8_752
Basic information of circular RNA
PanCircBase ID:
mmu_circSeptin8_752
mm39_Chr. Position ID:
chr11|53426774|53428742|+
Gene name:
Septin8
Transcript:
ENSMUST00000108987.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,704,1206,1539,1777
Genomic length:
1968
Exon/intron sizes in nt:
1,62,97,16,91,33,191
Flanking exon/introns:
chr11:53425962-53426774|chr11:53428742-53432036
Splice length:
752
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_351906
Human Ortholog IDs:
Mature spliced Sequence:
GTGAATATAATCCCCATCATTGCCAAGGCTGACACCATCTCCAAGAGCGAGCTCCACAAGTTCAAAATCAAGATCATGGGTGAGCTGGTCAGCAACGGAGTCCAGATCTACCAGTTTCCCACCGATGACGAGGCTGTCGCCGAGATTAATGCAGTCATGAACGCACACCTGCCTTTTGCCGTGGTGGGCAGCACAGAGGAGGTGAAGGTGGGGAATAAGCTGGTTCGAGCACGACAGTACCCTTGGGGTGTGGTGCAGGTGGAGAATGAGAATCACTGTGACTTCGTGAAGCTGCGGGAGATGCTGATCCGGGTGAACATGGAGGACCTGCGCGAGCAGACCCACAGCCGGCACTACGAGCTCTACCGCCGCTGCAAGTTGGAGGAGATGGGCTTCCAGGACAGTGACGGTGACAGCCAGCCCTTCAGCCTCCAAGAGACATATGAGGCAAAGAGGAAGGAGTTCCTAAGCGAGCTGCAGAGGAAGGAGGAAGAGATGCGACAGATGTTTGTCAACAAAGTCAAGGAGACAGAGCTTGAGCTGAAGGAGAAGGAACGGGAGCTCCATGAGAAGTTTGAGCACCTGAAGCGAATCCACCAGGAGGAGAAGCGCAAGGTAGAGGAGAAGCGCAGGGAGCTGGAAGAGGAGACCAACGCCTTCAACTGCCGGAAGGCGGCCATGGAGGCCCTGCAGTCACAGGCCTTGCATGCCACTTCACAGCAGCCTCTGAGGAAAGACAAGGACAAGAAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTTGCATGCCACTTCACAG
59.76
TGACCAGCTCACCCATGATC
59.46
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTTGCATGCCACTTCACAG
59.76
CTGACCAGCTCACCCATGAT
59.45
144
PCR template sequence:
ACGCCTTCAACTGCCGGAAGGCGGCCATGGAGGCCCTGCAGTCACAGGCCTTGCATGCCACTTCACAGCAGCCTCTGAGGAAAGACAAGGACAAGAAGAAGTGAATATAATCCCCATCATTGCCAAGGCTGACACCATCTCCAAGAGCGAGCTCCACAAGTTCAAAATCAAGATCATGGGTGAGCTGGTCAGCAACGGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-128-3p(1), mmu-miR-182-5p(1), mmu-miR-224-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
AGGACAAGAAGAAGTGAATATAATCC
siRNA sets
TATTCACTTCTTCTTGTCC, TATATTCACTTCTTCTTGT, ATTATATTCACTTCTTCTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSEPTIN8_752_ORF_1
1.231
0.687421544
0.991301218
681
ORF sequence:
ATGGGTGAGCTGGTCAGCAACGGAGTCCAGATCTACCAGTTTCCCACCGATGACGAGGCTGTCGCCGAGATTAATGCAGTCATGAACGCACACCTGCCTTTTGCCGTGGTGGGCAGCACAGAGGAGGTGAAGGTGGGGAATAAGCTGGTTCGAGCACGACAGTACCCTTGGGGTGTGGTGCAGGTGGAGAATGAGAATCACTGTGACTTCGTGAAGCTGCGGGAGATGCTGATCCGGGTGAACATGGAGGACCTGCGCGAGCAGACCCACAGCCGGCACTACGAGCTCTACCGCCGCTGCAAGTTGGAGGAGATGGGCTTCCAGGACAGTGACGGTGACAGCCAGCCCTTCAGCCTCCAAGAGACATATGAGGCAAAGAGGAAGGAGTTCCTAAGCGAGCTGCAGAGGAAGGAGGAAGAGATGCGACAGATGTTTGTCAACAAAGTCAAGGAGACAGAGCTTGAGCTGAAGGAGAAGGAACGGGAGCTCCATGAGAAGTTTGAGCACCTGAAGCGAATCCACCAGGAGGAGAAGCGCAAGGTAGAGGAGAAGCGCAGGGAGCTGGAAGAGGAGACCAACGCCTTCAACTGCCGGAAGGCGGCCATGGAGGCCCTGCAGTCACAGGCCTTGCATGCCACTTCACAGCAGCCTCTGAGGAAAGACAAGGACAAGAAGAAGTGA
Peptide sequence:
MGELVSNGVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVNKVKETELELKEKERELHEKFEHLKRIHQEEKRKVEEKRRELEEETNAFNCRKAAMEALQSQALHATSQQPLRKDKDKKK
IRESfinder result
IRESfinder score
0.192793374
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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