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mmu_circSenp7_852
Basic information of circular RNA
PanCircBase ID:
mmu_circSenp7_852
mm39_Chr. Position ID:
chr16|55971636|55982771|+
Gene name:
Senp7
Transcript:
ENSMUST00000089360.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,8,9,10,11
Exon/intron offsets:
0,2101,3950,7194,11101
Genomic length:
11135
Exon/intron sizes in nt:
185,334,152,147,34
Flanking exon/introns:
chr16:55959463-55971636|chr16:55982771-55986195
Splice length:
852
Other database IDs:
circAtlas: mmu-Senp7_0018
Human Ortholog IDs:
Mature spliced Sequence:
ACTTTAATACTGGAAATAAAGGTTGTGATTATCTTGAAGAGGGGACCAGCAACAAGAATACTTCATATTCTTATTCGGAAATGGACCATACTCCAGTTTCCAGGAAGAGAAAGAAAAGGGGTAGAAGTAATTTTCATGATTCTCATAATTCTAAGACTTCTTTGGATAAGCCAACAGAACATACAAAAGAAGAAGAAAATGACTCTTCAGTCTCCCGTAAGCTTGAAGAGTCCGGTGAAGACTCTCACCAGGACCCAGCACCACCCGAAGGACTCGCGCCAGAATCTTTAGAGAGCGAGGCCACTAACCTGCGCTCATTCGCTGCTGGTCAGGAGCCAGATGCTAGCGCTGCCTCTGGAAGGGCCTCTTCACCCAACAAAAGCTTGGAGAGCTCTGCTTCTAGTGAAGTGTCGGAGAATTCTTCCGTGGCTGTGAAGGGTGAGGCGTTGACTCTAAAAGAAGCATCCCCTCCGGGTGGAAGCAGTGAAGAGAGCCAGTTGTTGATCTCAGCTGAGCCAATTGTAGTTTCCAGTGATGAAGAAGGACCGGTTGAACATAAGAATTCAGTAATTCTAAAATTACAGCCTCCTCATGAGATTATGAGTGAAAATCAGGGCACTTCTGACCCACAGTTGTCAGAGCTGACACTCGGTGCATGTGAATCTGTACAGGTAACATCTGAATTATTTCCATACAATCCTGATGTAGAAAACATTTCCTGTATTAAATCTAATAGTGAAATGGATCTGAAATTGGATTTCATATTTACATGTGTGTATATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAAAATCCCGTTTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCAAAAGGTTGTGTCACGT
58.99
ACTGGAGTATGGTCCATTTCCG
59.83
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCCAAAAGGTTGTGTCACG
58.99
ACTGGAGTATGGTCCATTTCCG
59.83
152
PCR template sequence:
TTGGATTTCATATTTACATGTGTGTATATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAAAATCCCGTTTCAAGACTTTAATACTGGAAATAAAGGTTGTGATTATCTTGAAGAGGGGACCAGCAACAAGAATACTTCATATTCTTATTCGGAAATGGACCATACTCCAGTTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-216a-5p(1), mmu-miR-24-3p(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCCCGTTTCAAGACTTTAATACTGG
siRNA sets
TATTAAAGTCTTGAAACGG, ATTAAAGTCTTGAAACGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSENP7_852_ORF_1
1.0569
0.059055209
0.999822451
1623
ORF sequence:
ATGGACCATACTCCAGTTTCCAGGAAGAGAAAGAAAAGGGGTAGAAGTAATTTTCATGATTCTCATAATTCTAAGACTTCTTTGGATAAGCCAACAGAACATACAAAAGAAGAAGAAAATGACTCTTCAGTCTCCCGTAAGCTTGAAGAGTCCGGTGAAGACTCTCACCAGGACCCAGCACCACCCGAAGGACTCGCGCCAGAATCTTTAGAGAGCGAGGCCACTAACCTGCGCTCATTCGCTGCTGGTCAGGAGCCAGATGCTAGCGCTGCCTCTGGAAGGGCCTCTTCACCCAACAAAAGCTTGGAGAGCTCTGCTTCTAGTGAAGTGTCGGAGAATTCTTCCGTGGCTGTGAAGGGTGAGGCGTTGACTCTAAAAGAAGCATCCCCTCCGGGTGGAAGCAGTGAAGAGAGCCAGTTGTTGATCTCAGCTGAGCCAATTGTAGTTTCCAGTGATGAAGAAGGACCGGTTGAACATAAGAATTCAGTAATTCTAAAATTACAGCCTCCTCATGAGATTATGAGTGAAAATCAGGGCACTTCTGACCCACAGTTGTCAGAGCTGACACTCGGTGCATGTGAATCTGTACAGGTAACATCTGAATTATTTCCATACAATCCTGATGTAGAAAACATTTCCTGTATTAAATCTAATAGTGAAATGGATCTGAAATTGGATTTCATATTTACATGTGTGTATATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAAAATCCCGTTTCAAGACTTTAATACTGGAAATAAAGGTTGTGATTATCTTGAAGAGGGGACCAGCAACAAGAATACTTCATATTCTTATTCGGAAATGGACCATACTCCAGTTTCCAGGAAGAGAAAGAAAAGGGGTAGAAGTAATTTTCATGATTCTCATAATTCTAAGACTTCTTTGGATAAGCCAACAGAACATACAAAAGAAGAAGAAAATGACTCTTCAGTCTCCCGTAAGCTTGAAGAGTCCGGTGAAGACTCTCACCAGGACCCAGCACCACCCGAAGGACTCGCGCCAGAATCTTTAGAGAGCGAGGCCACTAACCTGCGCTCATTCGCTGCTGGTCAGGAGCCAGATGCTAGCGCTGCCTCTGGAAGGGCCTCTTCACCCAACAAAAGCTTGGAGAGCTCTGCTTCTAGTGAAGTGTCGGAGAATTCTTCCGTGGCTGTGAAGGGTGAGGCGTTGACTCTAAAAGAAGCATCCCCTCCGGGTGGAAGCAGTGAAGAGAGCCAGTTGTTGATCTCAGCTGAGCCAATTGTAGTTTCCAGTGATGAAGAAGGACCGGTTGAACATAAGAATTCAGTAATTCTAAAATTACAGCCTCCTCATGAGATTATGAGTGAAAATCAGGGCACTTCTGACCCACAGTTGTCAGAGCTGACACTCGGTGCATGTGAATCTGTACAGGTAACATCTGAATTATTTCCATACAATCCTGATGTAGAAAACATTTCCTGTATTAAATCTAATAGTGAAATGGATCTGAAATTGGATTTCATATTTACATGTGTGTATATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAAAATCCCGTTTCAA
Peptide sequence:
MDHTPVSRKRKKRGRSNFHDSHNSKTSLDKPTEHTKEEENDSSVSRKLEESGEDSHQDPAPPEGLAPESLESEATNLRSFAAGQEPDASAASGRASSPNKSLESSASSEVSENSSVAVKGEALTLKEASPPGGSSEESQLLISAEPIVVSSDEEGPVEHKNSVILKLQPPHEIMSENQGTSDPQLSELTLGACESVQVTSELFPYNPDVENISCIKSNSEMDLKLDFIFTCVYIGKIKGTPKGCVTFTKKYIKIPFQDFNTGNKGCDYLEEGTSNKNTSYSYSEMDHTPVSRKRKKRGRSNFHDSHNSKTSLDKPTEHTKEEENDSSVSRKLEESGEDSHQDPAPPEGLAPESLESEATNLRSFAAGQEPDASAASGRASSPNKSLESSASSEVSENSSVAVKGEALTLKEASPPGGSSEESQLLISAEPIVVSSDEEGPVEHKNSVILKLQPPHEIMSENQGTSDPQLSELTLGACESVQVTSELFPYNPDVENISCIKSNSEMDLKLDFIFTCVYIGKIKGTPKGCVTFTKKYIKIPFQ
IRESfinder result
IRESfinder score
0.646009633
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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