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mmu_circRnf130_251
Basic information of circular RNA
PanCircBase ID:
mmu_circRnf130_251
mm39_Chr. Position ID:
chr11|49961997|49962248|+
Gene name:
Rnf130
Transcript:
ENSMUST00000054684.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
251
Exon/intron sizes in nt:
251
Flanking exon/introns:
chr11:49943797-49961997|chr11:49962248-49976567
Splice length:
251
Other database IDs:
circAtlas: mmu-Rnf130_0007
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_153677
Human Ortholog IDs:
Mature spliced Sequence:
GCACGGGAGATATTATTGCTGTCATGATAACAGAATTGAGGGGTAAGGATATTTTGAGTTATCTGGAGAAAAACATCTCTGTACAAATGACAATAGCTGTTGGAACTCGAATGCCGCCAAAGAACTTCAGCCGTGGCTCTCTAGTCTTCGTGTCAATCTCCTTTATTGTTTTGATGATTATTTCTTCAGCGTGGCTCATATTCTACTTCATTCAGAAGATCAGGTACACAAATGCTCGGGACAGGAACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTTCTTCAGCGTGGCTCAT
57.88
TGTACAGAGATGTTTTTCTCCAGA
57.58
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTTCTTCAGCGTGGCTCAT
57.88
ACAGCTATTGTCATTTGTACAGAGA
58.3
172
PCR template sequence:
GTCAATCTCCTTTATTGTTTTGATGATTATTTCTTCAGCGTGGCTCATATTCTACTTCATTCAGAAGATCAGGTACACAAATGCTCGGGACAGGAACCAGGCACGGGAGATATTATTGCTGTCATGATAACAGAATTGAGGGGTAAGGATATTTTGAGTTATCTGGAGAAAAACATCTCTGTACAAATGACAATAGCTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
GGACAGGAACCAGGCACGGGAGATAT
siRNA sets
TCTCCCGTGCCTGGTTCCT, ATCTCCCGTGCCTGGTTCC, ATATCTCCCGTGCCTGGTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCRNF130_251_ORF_1
0.9844
-0.009157448
0.164999446
255
ORF sequence:
ATGATAACAGAATTGAGGGGTAAGGATATTTTGAGTTATCTGGAGAAAAACATCTCTGTACAAATGACAATAGCTGTTGGAACTCGAATGCCGCCAAAGAACTTCAGCCGTGGCTCTCTAGTCTTCGTGTCAATCTCCTTTATTGTTTTGATGATTATTTCTTCAGCGTGGCTCATATTCTACTTCATTCAGAAGATCAGGTACACAAATGCTCGGGACAGGAACCAGGCACGGGAGATATTATTGCTGTCATGA
Peptide sequence:
MITELRGKDILSYLEKNISVQMTIAVGTRMPPKNFSRGSLVFVSISFIVLMIISSAWLIFYFIQKIRYTNARDRNQAREILLLS
IRESfinder result
IRESfinder score
0.855472323
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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