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mmu_circRian_489
Basic information of circular RNA
PanCircBase ID:
mmu_circRian_489
mm39_Chr. Position ID:
chr12|109615587|109620866|+
Gene name:
Rian
Transcript:
ENSMUST00000238468.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
17,18,19,20,21
Exon/intron offsets:
0,918,1859,2705,5132
Genomic length:
5279
Exon/intron sizes in nt:
89,86,87,80,147
Flanking exon/introns:
chr12:109613185-109615587|chr12:109620866-109622282
Splice length:
489
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AATGCATTCTTCCACCAAGATGATTTACACAGCCTAGCTGGATCAGGAGCCCCCTAATAAGCTGCTTCAGATTGTAATATAGACACAGTAATGTATGCCTGCAGGATGAGTCCCACCTTCTTCATTAGTCTTGAGGTTTGAGATTCCCTGTTTGTGTCTGCAGGATTCAGATAGTAATGGATATCCTGCAAGTCGGCTTCGTAGAGTCTCCCTTGAAAGTGGACCTCATGATCTAGCCTTTATGTCCGTGGATTGACACTGTGATGGATTCCCCCCAAGTTCATTTAGATATTCTCTCTTATTCATAAATGTCACGGTCAGCTCTGTTCTTATAGATACCAGAATGGATACCCCTAAGCTGATGTATATACACCCTTCCTCGGTTGTGAGATTCCTGCTCTGGTGGTTGTAGTCATAAGATATACATCGTTGCGTACACCTTGGTTGTGGGCACGCACACTACTGTTGAATTACCACCTTGTCCAAGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGCTCTGGTGGTTGTAGT
59.31
GCAGCTTATTAGGGGGCTCC
60.25
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTCCTGCTCTGGTGGTTGT
59.23
GCAGCTTATTAGGGGGCTCC
60.25
164
PCR template sequence:
GATTCCTGCTCTGGTGGTTGTAGTCATAAGATATACATCGTTGCGTACACCTTGGTTGTGGGCACGCACACTACTGTTGAATTACCACCTTGTCCAAGTGAATGCATTCTTCCACCAAGATGATTTACACAGCCTAGCTGGATCAGGAGCCCCCTAATAAGCTGCTTCAGATTGTAATATAGACACAGTAATGTATGCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-690(1)
siRNAs for circular RNA silencing
Junction sequence
CCTTGTCCAAGTGAATGCATTCTTCC
siRNA sets
GAAGAATGCATTCACTTGG, AAGAATGCATTCACTTGGA, AGAATGCATTCACTTGGAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.734524736
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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