top of page
mmu_circRasgrf2_195
Basic information of circular RNA
PanCircBase ID:
mmu_circRasgrf2_195
mm39_Chr. Position ID:
chr13|92117065|92120404|-
Gene name:
Rasgrf2
Transcript:
ENSMUST00000151408.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
17,16
Exon/intron offsets:
0,3234
Genomic length:
3339
Exon/intron sizes in nt:
90,105
Flanking exon/introns:
chr13:92068050-92117065|chr13:92120404-92130712
Splice length:
195
Other database IDs:
circBase: mmu_circ_0004873, CIRCpedia: MMU_CIRCpedia_34256, circAtlas: mmu-Rasgrf2_0006
circBase: hsa_circ_0008315, circAtlas: hsa-RASGRF2_0021, CIRCpedia: HSA_CIRCpedia_52832
Human Ortholog IDs:
Mature spliced Sequence:
CTGTCTTGGAGTCTGCACCAGCCGACAAAGCGGGAGATTCTGCAGACATGTCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCAGCCTGGAGGACAGGTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCTGCAGCAGGACATGGGAGTCCACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTTTGCAATCGCCACAGCT
60.04
GGGGAGTTGTGGGGGATCTA
60.33
123
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGTCACCTGCTTCTGCTT
59.82
GGGGAGTTGTGGGGGATCTA
60.33
140
PCR template sequence:
CCTGGAGGACAGGTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCTGCAGCAGGACATGGGAGTCCACCAGCTGTCTTGGAGTCTGCACCAGCCGACAAAGCGGGAGATTCTGCAGACATGTCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-339-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGAGTCCACCAGCTGTCTTGGAGTC
siRNA sets
GACTCCAAGACAGCTGGTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCRASGRF2_195_ORF_1
1.3455
0.161096
0.59304796
342
ORF sequence:
ATGTCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCAGCCTGGAGGACAGGTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCTGCAGCAGGACATGGGAGTCCACCAGCTGTCTTGGAGTCTGCACCAGCCGACAAAGCGGGAGATTCTGCAGACATGTCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCAGCCTGGAGGACAGGTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCTGCAGCAGGACATGGGAGTCCACCA
Peptide sequence:
MSPCRSPTTPRHLRYRQPGGQVADSAHCSVSPASAFAIATAAAGHGSPPAVLESAPADKAGDSADMSPCRSPTTPRHLRYRQPGGQVADSAHCSVSPASAFAIATAAAGHGSPP
IRESfinder result
IRESfinder score
0.406888575
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page