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mmu_circRasal2_2599
Basic information of circular RNA
PanCircBase ID:
mmu_circRasal2_2599
mm39_Chr. Position ID:
chr1|156984475|157006822|-
Gene name:
Rasal2
Transcript:
ENSMUST00000078308.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
14,13,12,11,10,9,8,7,6
Exon/intron offsets:
0,1753,4219,6797,7701,17237,18757,20740,22193
Genomic length:
22347
Exon/intron sizes in nt:
8,62,20,02,02,23,71,45,00,00,00,00,000
Flanking exon/introns:
chr1:156977477-156984475|chr1:157006822-157020316
Splice length:
2599
Other database IDs:
CIRCpedia: MMU_CIRCpedia_216931, circAtlas: mmu-Rasal2_0041
Human Ortholog IDs:
Mature spliced Sequence:
GTCTCGAGGTCTGCCTAAACTGAAAGAGTCGCGTTCCCATGAGTCCCTGCTGAGCCCATGCAGTGCAGTGGAGTGTCTGGATCTAGGTAGAGGAGAGCCAGTCTCAGTGAAGCCACTGCACAGTAGCATCCTCGGACAGGACTTCTGCTTCGAGGTCACCTACTTAAGTGGAAGTAAATGCTTTAGCTGTAGCTCTGCCTCCGAGAGAGATAAGTGGATGGAAAACCTTCGAAGGACCGTTCAGCCAAATAAGGACAATTGTAGACGAGCTGAAAATGTTCTCCGTTTATGGATCATTGAAGCCAAGGACCTTGCCCCTAAAAAAAAATATTTCTGCGAACTGTGCCTTGATGATACCCTCTTTGCTCGTACAACCAGCAAGACCAAAGCAGACAATATTTTTTGGGGTGAACATTTTGAATTCTATAGCCTTCCACCTCTTCATAGCATCACGGTTCATATTTATAAGGATGTGGAAAAAAAGAAAAAAAAGGACAAGAATAATTATGTAGGGCTAGTCAATATCCCCACTGCCAGTGTGACTGGTCGCCAGTTTGTAGAAAAGTGGTACCCAGTGAGTACACCCACACCCAACAAAGGAAAGACAGGAGGACCTTCTATTCGGATTAAGTCACGTTTCCAAACTATCACCATTCTGCCTATGGAGCAATACAAAGAGTTTGCAGAATTCATCACCAGCAACTACACCATGCTGTGTTCTGTTCTTGAGCCAGTAATTAGTGTGAGGAATAAAGAGGAGTTGGCTTGTGCCTTAGTACACATTCTTCAAAGTACTGGCAGAGCCAAGGATTTTCTGACTGACTTGGTGATGTCAGAGGTAGATCGTTGTGGAGAACATGATGTCTTGATCTTCAGAGAAAACACCATTGCCACTAAATCCATTGAGGAATACCTCAAGTTGGTGGGACAACAGTACCTTCATGATGCACTCGGGGAGTTCATCAAAGCCTTGTATGAATCAGATGAGAACTGTGAAGTGGATCCCAGCAAGTGTTCATCTAGTGAACTGATGGACCATCAGAGCAACCTGAAAATGTGCTGTGAGTTGGCTTTCTGCAAGATCATCAACTCATACTGTGTCTTCCCGCGTGAGTTGAAAGAAGTGTTTGCATCATGGAAGCAGCAGTGCCTGAACCGAGGCAAGCAAGACATCAGTGAGCGGCTCATCAGTGCCTCATTGTTTCTCCGTTTTCTTTGCCCAGCCATTATGTCTCCCAGTCTTTTCAACCTGATGCAGGAATATCCTGATGACCGCACATCTCGGACACTAACTCTTATTGCCAAGGTCATTCAGAACCTGGCCAACTTTGCCAAGTTTGGTAACAAAGAGGAGTACATGGCATTCATGAATGATTTTTTAGAACATGAATGGGGTGGAATGAAGCGGTTCCTTTTGGAGATCTCTAATCCTGACACCATCTCAAACACCCCAGGCTTCGATGGTTACATTGATTTGGGCAGAGAGCTGTCAGTTTTGCATTCCTTACTATGGGAAGTAGTTTCCCAACTTGATAAGGGTGAAAATTCCTTTCTACAGGCAACTGTGGCAAAACTGGGACCTCTCCCTCGTGTTCTTGCTGACATTACCAAGTCTCTGACTAATCCTACGCCAATACAGCAGCAGCTAAGACGTTTCGCTGAGCACAGCTCCAGTCCAAACGTCAGCGGAAGCCTCTCCTCTGGGCTGCAGAGAATATGTGAAGACCCCACTGACAGTGATTTGCATAAACTAAAGTCTCCAAGCCAGGATAACACAGACAGCTATTTCAGAGGAAAGACGTTATTGTTGGTTCAGCAAGCCTCCTCCCAGAGCATGACGTACTCTGAAAAGGATGAAAAGGAAAACAGCCTTCCTAACGGTCGGAGCATCTCCCTCATGGACCTCCAGGACACTCATGCTGCTCAGGCGGAGCATGCATCTGTCATGCTTGATGTGCCTATGCGCCTTGCAGGAAGCCAGCTTTCTATAACCCAGGTAGCTAGCATCAAACAGCTGCGGGAAACCCAGAGCACTCCCCAGAGTGCACCTCAAGTGAGAAGACCCCTGCACCCAGCCTTGAACCAGCCAGGAAGTCTCCAGCCCTTGTCATTCCAGAATCCTGTCTATCACCTCAACAACCCAGTTCCAGCAATGCCAAAGGCCTCTGCGGATTCCAGTTTGGAGAACCTAAGCACTGCCAGTTCTAGAAGCCAAAGCAATAGTGAAGACTTCAAACTCAGTGGACCCAGCAATAGCAGCATGGAAGATTTCACCAAAAGAAGCTCCCACAGTGAAGACTTCTCCAGGCGCCACACTGTACCAGACAGACACATACCTCTTGCTCTACCACGACAAAATAGTACGGGGCAGTCCCAAATCCGAAAACTGGACCATTCTGGGTTAGGTGCCCGAGCCAAAGCACCCCCATCTCTGCCACACAGTGCTTCCTTGCGTAGCACAGGGAGCATGTCAGTGGCGTCTGCAGCCCTAATGGCTGAACCTGTGCAGAACGGGAGCCGGTCCAGGCAGCAGTCCTCTTCCTCCAGAGAGAGCCCTGTTCCCAAAGTGAGAGCAATCCAGAGACAACAGACACAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTCCTCCAGAGAGAGCCCT
60.03
ATCCAGACACTCCACTGCAC
59.68
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTCCTCCAGAGAGAGCCC
60.03
ATCCAGACACTCCACTGCAC
59.68
145
PCR template sequence:
ACCTGTGCAGAACGGGAGCCGGTCCAGGCAGCAGTCCTCTTCCTCCAGAGAGAGCCCTGTTCCCAAAGTGAGAGCAATCCAGAGACAACAGACACAACAGGTCTCGAGGTCTGCCTAAACTGAAAGAGTCGCGTTCCCATGAGTCCCTGCTGAGCCCATGCAGTGCAGTGGAGTGTCTGGATCTAGGTAGAGGAGAGCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
25
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(2), mmu-miR-431-5p(2), mmu-miR-9-5p(1), mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-miR-322-5p(1), mmu-miR-217-5p(1), mmu-miR-7a-5p(1), mmu-miR-709(1), mmu-miR-320-3p(1), mmu-miR-326-3p(1), mmu-miR-138-5p(1), mmu-miR-214-3p(1), mmu-miR-182-5p(1), mmu-miR-511-3p(1), mmu-miR-34b-5p(1), mmu-miR-107-3p(1), mmu-miR-300-3p(1), mmu-miR-5101(1), mmu-miR-3087-3p(1), mmu-miR-670-5p(1), mmu-miR-697(1), mmu-miR-202-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACAGACACAACAGGTCTCGAGGTCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCRASAL2_2599_ORF_1
0.9051
0.168401094
0.9999994
2400
ORF sequence:
ATGGAAAACCTTCGAAGGACCGTTCAGCCAAATAAGGACAATTGTAGACGAGCTGAAAATGTTCTCCGTTTATGGATCATTGAAGCCAAGGACCTTGCCCCTAAAAAAAAATATTTCTGCGAACTGTGCCTTGATGATACCCTCTTTGCTCGTACAACCAGCAAGACCAAAGCAGACAATATTTTTTGGGGTGAACATTTTGAATTCTATAGCCTTCCACCTCTTCATAGCATCACGGTTCATATTTATAAGGATGTGGAAAAAAAGAAAAAAAAGGACAAGAATAATTATGTAGGGCTAGTCAATATCCCCACTGCCAGTGTGACTGGTCGCCAGTTTGTAGAAAAGTGGTACCCAGTGAGTACACCCACACCCAACAAAGGAAAGACAGGAGGACCTTCTATTCGGATTAAGTCACGTTTCCAAACTATCACCATTCTGCCTATGGAGCAATACAAAGAGTTTGCAGAATTCATCACCAGCAACTACACCATGCTGTGTTCTGTTCTTGAGCCAGTAATTAGTGTGAGGAATAAAGAGGAGTTGGCTTGTGCCTTAGTACACATTCTTCAAAGTACTGGCAGAGCCAAGGATTTTCTGACTGACTTGGTGATGTCAGAGGTAGATCGTTGTGGAGAACATGATGTCTTGATCTTCAGAGAAAACACCATTGCCACTAAATCCATTGAGGAATACCTCAAGTTGGTGGGACAACAGTACCTTCATGATGCACTCGGGGAGTTCATCAAAGCCTTGTATGAATCAGATGAGAACTGTGAAGTGGATCCCAGCAAGTGTTCATCTAGTGAACTGATGGACCATCAGAGCAACCTGAAAATGTGCTGTGAGTTGGCTTTCTGCAAGATCATCAACTCATACTGTGTCTTCCCGCGTGAGTTGAAAGAAGTGTTTGCATCATGGAAGCAGCAGTGCCTGAACCGAGGCAAGCAAGACATCAGTGAGCGGCTCATCAGTGCCTCATTGTTTCTCCGTTTTCTTTGCCCAGCCATTATGTCTCCCAGTCTTTTCAACCTGATGCAGGAATATCCTGATGACCGCACATCTCGGACACTAACTCTTATTGCCAAGGTCATTCAGAACCTGGCCAACTTTGCCAAGTTTGGTAACAAAGAGGAGTACATGGCATTCATGAATGATTTTTTAGAACATGAATGGGGTGGAATGAAGCGGTTCCTTTTGGAGATCTCTAATCCTGACACCATCTCAAACACCCCAGGCTTCGATGGTTACATTGATTTGGGCAGAGAGCTGTCAGTTTTGCATTCCTTACTATGGGAAGTAGTTTCCCAACTTGATAAGGGTGAAAATTCCTTTCTACAGGCAACTGTGGCAAAACTGGGACCTCTCCCTCGTGTTCTTGCTGACATTACCAAGTCTCTGACTAATCCTACGCCAATACAGCAGCAGCTAAGACGTTTCGCTGAGCACAGCTCCAGTCCAAACGTCAGCGGAAGCCTCTCCTCTGGGCTGCAGAGAATATGTGAAGACCCCACTGACAGTGATTTGCATAAACTAAAGTCTCCAAGCCAGGATAACACAGACAGCTATTTCAGAGGAAAGACGTTATTGTTGGTTCAGCAAGCCTCCTCCCAGAGCATGACGTACTCTGAAAAGGATGAAAAGGAAAACAGCCTTCCTAACGGTCGGAGCATCTCCCTCATGGACCTCCAGGACACTCATGCTGCTCAGGCGGAGCATGCATCTGTCATGCTTGATGTGCCTATGCGCCTTGCAGGAAGCCAGCTTTCTATAACCCAGGTAGCTAGCATCAAACAGCTGCGGGAAACCCAGAGCACTCCCCAGAGTGCACCTCAAGTGAGAAGACCCCTGCACCCAGCCTTGAACCAGCCAGGAAGTCTCCAGCCCTTGTCATTCCAGAATCCTGTCTATCACCTCAACAACCCAGTTCCAGCAATGCCAAAGGCCTCTGCGGATTCCAGTTTGGAGAACCTAAGCACTGCCAGTTCTAGAAGCCAAAGCAATAGTGAAGACTTCAAACTCAGTGGACCCAGCAATAGCAGCATGGAAGATTTCACCAAAAGAAGCTCCCACAGTGAAGACTTCTCCAGGCGCCACACTGTACCAGACAGACACATACCTCTTGCTCTACCACGACAAAATAGTACGGGGCAGTCCCAAATCCGAAAACTGGACCATTCTGGGTTAGGTGCCCGAGCCAAAGCACCCCCATCTCTGCCACACAGTGCTTCCTTGCGTAGCACAGGGAGCATGTCAGTGGCGTCTGCAGCCCTAATGGCTGAACCTGTGCAGAACGGGAGCCGGTCCAGGCAGCAGTCCTCTTCCTCCAGAGAGAGCCCTGTTCCCAAAGTGAGAGCAATCCAGAGACAACAGACACAACAGGTCTCGAGGTCTGCCTAA
Peptide sequence:
MENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFITSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELMDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFAEHSSSPNVSGSLSSGLQRICEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKENSLPNGRSISLMDLQDTHAAQAEHASVMLDVPMRLAGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPVPAMPKASADSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSSHSEDFSRRHTVPDRHIPLALPRQNSTGQSQIRKLDHSGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVSRSA
IRESfinder result
IRESfinder score
0.626913434
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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