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mmu_circRanbp17_324
Basic information of circular RNA
PanCircBase ID:
mmu_circRanbp17_324
mm39_Chr. Position ID:
chr11|33441326|33450770|-
Gene name:
Ranbp17
Transcript:
ENSMUST00000102815.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,1924,9353
Genomic length:
9444
Exon/intron sizes in nt:
66,167,91
Flanking exon/introns:
chr11:33437747-33441326|chr11:33450770-33454681
Splice length:
324
Other database IDs:
circAtlas: mmu-Ranbp17_0044
circBase: hsa_circ_0128575, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_51125
Human Ortholog IDs:
Mature spliced Sequence:
ACATCCTATGCTCAGCTCCTTGCAGCAACATGTCTTTCAAAACTTGTTACCCGAATCAATCCTTTACCTATTGAACAGAGGATAGACATCAGAAACTACATCCTGAATTATGTGGCGTCCCAACCTAAGCTGGCGCCCTTTGTCATCCAAGCTCTGATTCAAGTCATCGCTAAACTCACCAAGTTGGGGTGGTTTGAGGTGCAGAAGGATGAGTTTGTTTTCCGAGAAATCATTGCTGATGTGAAGAAGTTCCTCCAGGGCACGGTGGAACACTGCATAATAGGAGTGATAATCCTTTGTGAATTGACACAGGAAATGAACTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGAAGTTCCTCCAGGGCAC
59.6
AGGTAAAGGATTGATTCGGGT
56.69
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGAAGTTCCTCCAGGGCAC
59.6
AGGATTGATTCGGGTAACAAGT
57.43
144
PCR template sequence:
AGAAATCATTGCTGATGTGAAGAAGTTCCTCCAGGGCACGGTGGAACACTGCATAATAGGAGTGATAATCCTTTGTGAATTGACACAGGAAATGAACTTGACATCCTATGCTCAGCTCCTTGCAGCAACATGTCTTTCAAAACTTGTTACCCGAATCAATCCTTTACCTATTGAACAGAGGATAGACATCAGAAACTACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-23a-3p(1), mmu-miR-495-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAAATGAACTTGACATCCTATGCTC
siRNA sets
ATAGGATGTCAAGTTCATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCRANBP17_324_ORF_1
1.0051
0.219479711
0.477828049
333
ORF sequence:
ATGAACTTGACATCCTATGCTCAGCTCCTTGCAGCAACATGTCTTTCAAAACTTGTTACCCGAATCAATCCTTTACCTATTGAACAGAGGATAGACATCAGAAACTACATCCTGAATTATGTGGCGTCCCAACCTAAGCTGGCGCCCTTTGTCATCCAAGCTCTGATTCAAGTCATCGCTAAACTCACCAAGTTGGGGTGGTTTGAGGTGCAGAAGGATGAGTTTGTTTTCCGAGAAATCATTGCTGATGTGAAGAAGTTCCTCCAGGGCACGGTGGAACACTGCATAATAGGAGTGATAATCCTTTGTGAATTGACACAGGAAATGAACTTG
Peptide sequence:
MNLTSYAQLLAATCLSKLVTRINPLPIEQRIDIRNYILNYVASQPKLAPFVIQALIQVIAKLTKLGWFEVQKDEFVFREIIADVKKFLQGTVEHCIIGVIILCELTQEMNL
IRESfinder result
IRESfinder score
0.673233613
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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