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mmu_circQrich1_1810
Basic information of circular RNA
PanCircBase ID:
mmu_circQrich1_1810
mm39_Chr. Position ID:
chr9|108405787|108422176|+
Gene name:
Qrich1
Transcript:
ENSMUST00000112155.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,4998,13099,13843,16274
Genomic length:
16389
Exon/intron sizes in nt:
330,1032,178,155,115
Flanking exon/introns:
chr9:108394439-108405787|chr9:108422176-108433160
Splice length:
1810
Other database IDs:
circBase: mmu_circ_0015257, CIRCpedia: MMU_CIRCpedia_2398, circAtlas: mmu-Qrich1_0002
circBase: hsa_circ_0124033, circAtlas: hsa-QRICH1_0004, CIRCpedia: HSA_CIRCpedia_46091
Human Ortholog IDs:
Mature spliced Sequence:
GAACCCTAAGGACTCTGCAATATGAATAATTCTCTAGAGAACACCATCTCATTTGAAGAGTACATCCGAGTAAAGGCGAGGTCTGTCCCGCAACACAGGATGAAGGAATTTCTGGACTCACTGGCTTCTAAGGGACCAGAAGCTCTTCAGGAGTTCCAGCAGACTGCCACCACCACCATGGTGTACCAGCAGGGTGGGAACTGCATTTATACAGACAGCACTGAAGTGGCTGGGTCTTTGCTTGAACTTGCTTGTCCAGTCACAACCAGTGTTCAACCACAAACTCAGCAAGAGCAGCAGATCCAGGTTCAGCAGCCACAGCAGGTTCAGGTACAGGTGCAGGTGCAGCAGTCTCCACAACAGGTCTCAGCTCAGCAACTTTCCCCACAGTTCACCGTTCACCAGCCTGCTGAGCAACCCATCCAGGTCCAGGTGCAGATCCAGGGCCAAGCACCACAGTCCGCAGCCCCCTCCATTCAGACTCCATCTCTGCAGAGCCCCAGCCCCTCACAGCTGCAAGCAGCTCAGATCCAGGTGCAGCACGTCCAGGCGGCCCAACAGATCCAGGCTGCAGAAATCCCAGAGGAGCATATCCCACATCAACAAATCCAGGCTCAGTTGGTAGCTGGCCAATCTCTTGCTGGGGGCCAGCAAATTCAAATTCAGACTGTGGGTGCTCTTTCCCCACCACCATCCCAGCAGGGCTCACCCAGGGAAGGGGAACGGAGGGTTGGCACAGCCAGTGTCCTTCAGCCAGTGAAGAAACGCAAAGTGGACATGCCTATCACCGTGTCCTACGCCATCTCAGGGCAGCCAGTGGCCACTGTGCTGGCCATTCCACAAGGCCAGCAACAGAGTTATGTGTCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGGACCATAACTAGCCCTACAGGAGAAACTTGGACCATCCCTGTTTATTCTGCCCAGCCCCGCGGGGATCCTCAGCAGCAGAGCATTACGCACATTGCCATTCCCCAGGAAGCCTACAATGCGGTTCATGTCAGTGGCTCACCTACGGCCCTGGCAGCTGTAAAGCTGGAGGATGACAAGGAGAAGATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATGAAGAGGTAGTGCAGACCCTTGCAAACTCTCTCTTTCCAGCACAGTTCATGAATGGCAACATCCACATTCCAGTGGCTGTGCAGGCTGTAGCAGGCACATACCAGAATACGGCTCAGACTGTACATATATGGGACCCACAGCAGCAGCCACAACAGCAAACTGCACAAGAGCAGACACCACCACCACAACAGCAGCAGCAGCAGCTGCAGGTCACTTGTTCAGCACAGACTGTTCAGGTTGCTGAAGTTGAGCCACAGTCACAACCACAGCCTTCACCAGAGCTTTTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAAGTATGGAAAAACTGGGCTCAGACCAAGAATGCTGAACTAGAAAAGGATGCTCAAAACAGATTGGCCCCCATTGGGAGGCGACAGTTGCTACGATTTCAGGAAGATCTGATCTCCTCTGCTGTGGCTGAATTGAATTATGGCCTTTGTTTAATGACACGAGAAGCTCGGAATGGAGAAGGTGAACCCTATGACCCAGATGTACTCTACTACATTTTCCTGTGTATACAGAAGTATCTTTTTGAAAATGGAAGAGTAGATGACATATTTTCTGATCTTTACTATGTTCGTTTCACCGAATGGCTACATGAAGTTCTGAAGGATGTACAGCCTCGGGTCACTCCACTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGTTTCACCGAATGGCTACA
60
GGGACAGACCTCGCCTTTAC
60.11
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGTTTCACCGAATGGCTACA
60
CAGACCTCGCCTTTACTCGG
60.18
147
PCR template sequence:
GGAAGAGTAGATGACATATTTTCTGATCTTTACTATGTTCGTTTCACCGAATGGCTACATGAAGTTCTGAAGGATGTACAGCCTCGGGTCACTCCACTTGGAACCCTAAGGACTCTGCAATATGAATAATTCTCTAGAGAACACCATCTCATTTGAAGAGTACATCCGAGTAAAGGCGAGGTCTGTCCCGCAACACAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-433-5p(2), mmu-miR-370-3p(2), mmu-miR-758-3p(2), mmu-miR-210-3p(1), mmu-miR-134-5p(1), mmu-miR-206-3p(1), mmu-miR-328-3p(1), mmu-miR-298-5p(1), mmu-miR-138-5p(1), mmu-miR-24-3p(1), mmu-miR-351-5p(1), mmu-miR-22-3p(1), mmu-miR-383-5p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCACTCCACTTGGAACCCTAAGGAC
siRNA sets
TCCTTAGGGTTCCAAGTGG, GTCCTTAGGGTTCCAAGTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCQRICH1_1810_ORF_1
1.3301
0.239492583
0.999986919
1818
ORF sequence:
ATGAATAATTCTCTAGAGAACACCATCTCATTTGAAGAGTACATCCGAGTAAAGGCGAGGTCTGTCCCGCAACACAGGATGAAGGAATTTCTGGACTCACTGGCTTCTAAGGGACCAGAAGCTCTTCAGGAGTTCCAGCAGACTGCCACCACCACCATGGTGTACCAGCAGGGTGGGAACTGCATTTATACAGACAGCACTGAAGTGGCTGGGTCTTTGCTTGAACTTGCTTGTCCAGTCACAACCAGTGTTCAACCACAAACTCAGCAAGAGCAGCAGATCCAGGTTCAGCAGCCACAGCAGGTTCAGGTACAGGTGCAGGTGCAGCAGTCTCCACAACAGGTCTCAGCTCAGCAACTTTCCCCACAGTTCACCGTTCACCAGCCTGCTGAGCAACCCATCCAGGTCCAGGTGCAGATCCAGGGCCAAGCACCACAGTCCGCAGCCCCCTCCATTCAGACTCCATCTCTGCAGAGCCCCAGCCCCTCACAGCTGCAAGCAGCTCAGATCCAGGTGCAGCACGTCCAGGCGGCCCAACAGATCCAGGCTGCAGAAATCCCAGAGGAGCATATCCCACATCAACAAATCCAGGCTCAGTTGGTAGCTGGCCAATCTCTTGCTGGGGGCCAGCAAATTCAAATTCAGACTGTGGGTGCTCTTTCCCCACCACCATCCCAGCAGGGCTCACCCAGGGAAGGGGAACGGAGGGTTGGCACAGCCAGTGTCCTTCAGCCAGTGAAGAAACGCAAAGTGGACATGCCTATCACCGTGTCCTACGCCATCTCAGGGCAGCCAGTGGCCACTGTGCTGGCCATTCCACAAGGCCAGCAACAGAGTTATGTGTCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGGACCATAACTAGCCCTACAGGAGAAACTTGGACCATCCCTGTTTATTCTGCCCAGCCCCGCGGGGATCCTCAGCAGCAGAGCATTACGCACATTGCCATTCCCCAGGAAGCCTACAATGCGGTTCATGTCAGTGGCTCACCTACGGCCCTGGCAGCTGTAAAGCTGGAGGATGACAAGGAGAAGATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATGAAGAGGTAGTGCAGACCCTTGCAAACTCTCTCTTTCCAGCACAGTTCATGAATGGCAACATCCACATTCCAGTGGCTGTGCAGGCTGTAGCAGGCACATACCAGAATACGGCTCAGACTGTACATATATGGGACCCACAGCAGCAGCCACAACAGCAAACTGCACAAGAGCAGACACCACCACCACAACAGCAGCAGCAGCAGCTGCAGGTCACTTGTTCAGCACAGACTGTTCAGGTTGCTGAAGTTGAGCCACAGTCACAACCACAGCCTTCACCAGAGCTTTTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAAGTATGGAAAAACTGGGCTCAGACCAAGAATGCTGAACTAGAAAAGGATGCTCAAAACAGATTGGCCCCCATTGGGAGGCGACAGTTGCTACGATTTCAGGAAGATCTGATCTCCTCTGCTGTGGCTGAATTGAATTATGGCCTTTGTTTAATGACACGAGAAGCTCGGAATGGAGAAGGTGAACCCTATGACCCAGATGTACTCTACTACATTTTCCTGTGTATACAGAAGTATCTTTTTGAAAATGGAAGAGTAGATGACATATTTTCTGATCTTTACTATGTTCGTTTCACCGAATGGCTACATGAAGTTCTGAAGGATGTACAGCCTCGGGTCACTCCACTTGGAACCCTAAGGACTCTGCAATATGAATAA
Peptide sequence:
MNNSLENTISFEEYIRVKARSVPQHRMKEFLDSLASKGPEALQEFQQTATTTMVYQQGGNCIYTDSTEVAGSLLELACPVTTSVQPQTQQEQQIQVQQPQQVQVQVQVQQSPQQVSAQQLSPQFTVHQPAEQPIQVQVQIQGQAPQSAAPSIQTPSLQSPSPSQLQAAQIQVQHVQAAQQIQAAEIPEEHIPHQQIQAQLVAGQSLAGGQQIQIQTVGALSPPPSQQGSPREGERRVGTASVLQPVKKRKVDMPITVSYAISGQPVATVLAIPQGQQQSYVSLRPDLLTVDSAHLYSATGTITSPTGETWTIPVYSAQPRGDPQQQSITHIAIPQEAYNAVHVSGSPTALAAVKLEDDKEKMVGTTSVVKNSHEEVVQTLANSLFPAQFMNGNIHIPVAVQAVAGTYQNTAQTVHIWDPQQQPQQQTAQEQTPPPQQQQQQLQVTCSAQTVQVAEVEPQSQPQPSPELLLPNSLKPEEGLEVWKNWAQTKNAELEKDAQNRLAPIGRRQLLRFQEDLISSAVAELNYGLCLMTREARNGEGEPYDPDVLYYIFLCIQKYLFENGRVDDIFSDLYYVRFTEWLHEVLKDVQPRVTPLGTLRTLQYE
IRESfinder result
IRESfinder score
0.577687288
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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