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mmu_circPus7_614
Basic information of circular RNA
PanCircBase ID:
mmu_circPus7_614
mm39_Chr. Position ID:
chr5|23980666|23983497|-
Gene name:
Pus7
Transcript:
ENSMUST00000119946.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,215,2404
Genomic length:
2831
Exon/intron sizes in nt:
1,02,85,427
Flanking exon/introns:
chr5:23973910-23980666|chr5:23983497-23988509
Splice length:
614
Other database IDs:
circBase: hsa_circ_0005705, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_56210
Human Ortholog IDs:
Mature spliced Sequence:
GTTCTCTTAAATGTTTTCTTGGAGCCTTAAAGATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCACACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGACGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAATTCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAAGAAGACGGCCTTTCAGAAGCAGGGGAAGAGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAAGTTCGTGAGTTCCCATCACGGCTTCTCGGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAAATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGATGAAGAGGACCCTCCAGAAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAAAGAAACCAGTGTTGCCATCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCCAGAAGACGCTCTCAC
59.83
TTTCAGGGACGTGCTTGTCA
59.82
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCCAGAAGACGCTCTCAC
59.83
CAACAAGGCACCCACGTTTC
60.25
174
PCR template sequence:
CCCTCCAGAAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAAAGAAACCAGTGTTGCCATCGAGGTTCTCTTAAATGTTTTCTTGGAGCCTTAAAGATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
TGTTGCCATCGAGGTTCTCTTAAATG
siRNA sets
TTTAAGAGAACCTCGATGG, ATTTAAGAGAACCTCGATG, TAAGAGAACCTCGATGGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPUS7_614_ORF_1
1.163
0.384674312
0.946854418
612
ORF sequence:
ATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCACACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGACGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAATTCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAAGAAGACGGCCTTTCAGAAGCAGGGGAAGAGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAAGTTCGTGAGTTCCCATCACGGCTTCTCGGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAAATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGATGAAGAGGACCCTCCAGAAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAAAGAAACCAGTGTTGCCATCGAGGTTCTCTTAAATGTTTTCTTGGAGCCTTAA
Peptide sequence:
MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDENSSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVLLNVFLEP
IRESfinder result
IRESfinder score
0.277385122
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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