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mmu_circPus10_442
Basic information of circular RNA
PanCircBase ID:
mmu_circPus10_442
mm39_Chr. Position ID:
chr11|23656884|23667618|+
Gene name:
Pus10
Transcript:
ENSMUST00000109525.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,8,9,10,11,12
Exon/intron offsets:
0,638,1873,4311,5315,10677
Genomic length:
10734
Exon/intron sizes in nt:
62,46,65,86,126,57
Flanking exon/introns:
chr11:23653001-23656884|chr11:23667618-23668575
Splice length:
442
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGCTTGTTCGAAGTGAGCGTGGTCTTTGCTCATCCAGAAACAGCTGAAGATTGCCATTTCCTAGGTGAAGTTTGCCGAGATTGTTTCAAACCAGCTAAAAATAAACAGTCGGTATTTACCAGAATGGCAGTTTTGAAAGCTTTGAGTAAGATAAAAGAAGAAGACTTCCTCGGGCAATTTCCTTGTCCTCCAAACTCACCGAAGACTGTATGCACTGTCCTTGAAGTTGAGTGCACTCATGGTGCTGTTTTTGTCGCTGGCAGATATAATAAATACTCCAGGAATCTACCACAAACTCCTTGGATAATTGATGGAGAAAGGAAAATGGAATCTTCGGTGGAAGAATTAATTTCAGATCATCTTCTGGCAGTATTCAGAGCAGAGAGTTTTAATTTCTCATCCTCGGGACGAGAAGACGTAGACGTGAGAACATTAGGAAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCAGTATTCAGAGCAGAGAG
59.57
CTCGGCAAACTTCACCTAGGA
59.72
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCAGTATTCAGAGCAGAGAG
59.57
TCTCGGCAAACTTCACCTAGG
59.72
158
PCR template sequence:
GAATTAATTTCAGATCATCTTCTGGCAGTATTCAGAGCAGAGAGTTTTAATTTCTCATCCTCGGGACGAGAAGACGTAGACGTGAGAACATTAGGAAATGAGCTTGTTCGAAGTGAGCGTGGTCTTTGCTCATCCAGAAACAGCTGAAGATTGCCATTTCCTAGGTGAAGTTTGCCGAGATTGTTTCAAACCAGCTAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-200c-3p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACATTAGGAAATGAGCTTGTTCGAAG
siRNA sets
TTCGAACAAGCTCATTTCC, TCGAACAAGCTCATTTCCT, CTTCGAACAAGCTCATTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPUS10_442_ORF_1
0.8847
-0.071206206
0.330564848
444
ORF sequence:
ATGAGCTTGTTCGAAGTGAGCGTGGTCTTTGCTCATCCAGAAACAGCTGAAGATTGCCATTTCCTAGGTGAAGTTTGCCGAGATTGTTTCAAACCAGCTAAAAATAAACAGTCGGTATTTACCAGAATGGCAGTTTTGAAAGCTTTGAGTAAGATAAAAGAAGAAGACTTCCTCGGGCAATTTCCTTGTCCTCCAAACTCACCGAAGACTGTATGCACTGTCCTTGAAGTTGAGTGCACTCATGGTGCTGTTTTTGTCGCTGGCAGATATAATAAATACTCCAGGAATCTACCACAAACTCCTTGGATAATTGATGGAGAAAGGAAAATGGAATCTTCGGTGGAAGAATTAATTTCAGATCATCTTCTGGCAGTATTCAGAGCAGAGAGTTTTAATTTCTCATCCTCGGGACGAGAAGACGTAGACGTGAGAACATTAGGAAAT
Peptide sequence:
MSLFEVSVVFAHPETAEDCHFLGEVCRDCFKPAKNKQSVFTRMAVLKALSKIKEEDFLGQFPCPPNSPKTVCTVLEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGERKMESSVEELISDHLLAVFRAESFNFSSSGREDVDVRTLGN
IRESfinder result
IRESfinder score
0.751112739
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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