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mmu_circPsme4_1710
Basic information of circular RNA
PanCircBase ID:
mmu_circPsme4_1710
mm39_Chr. Position ID:
chr11|30769003|30793588|+
Gene name:
Psme4
Transcript:
ENSMUST00000041231.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
16
Exon/intron indexes:
17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32
Exon/intron offsets:
0,924,1912,4055,11346,13130,13385,13528,15082,15270,16332,18278,19881,22565,22987,24503
Genomic length:
24585
Exon/intron sizes in nt:
68,216,158,96,56,57,56,99,63,96,132,210,137,54,130,82
Flanking exon/introns:
chr11:30768158-30769003|chr11:30793588-30795179
Splice length:
1710
Other database IDs:
circAtlas: mmu-Psme4_0068
Human Ortholog IDs:
Mature spliced Sequence:
ATGATGATGTATTGAATGAAGAAGAGCTAGACAAGGAGTTGCTGTGGAACCTTCAGCTTCTGTCTGAGATTACTCGTGTAGACGGGAAGAAGTTACTTCTGTATAGGGAGCAGCTTGTAAAGATTCTCCAGCGAACCTTACACCTGACCTGTAAGCAGGGCTACACGCTGTCTTGCAACCTTCTGCATCATCTTCTTCGTTCTACCACACTAATCTACCCTACAGAGTACTGCAGTGTTCCGGGGGGCTTTAACAAGCCGCCTTCTGAATACTTCCCTGTCAAGGACTGGGGTAAGCCTGGAGATTTGTGGAATCTGGGGATCCAGTGGCATGTTCCTTCTTCAGAAGAAGTCTCTTTTGCCTTCTATTTGTTAGACTCCTTTCTTCAGCCTGAGCTTATCAAACTCCAGTGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGTACACAGCTGCTTGATTGGTTCAGGGAACCTTCTACCTCCATTGAAAGGAGAGGCAGTTACTAACTTGGTACCAAGTATGGTCTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAGAGAAAACTACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATACCAAATCACTGTTTCTCATCATAAAGATCATTGGAGACCTTCTGCACTTCCAAGGATCTCATAAACATGAATTTGACTCTCGATGGAAAAGCTTTAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGCAACATATCAGAGCATTACTAATTGACAGAGTGATGCTGCAGCATGAGTTGCGAACACTAACTGTTGAGGGTTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGCTCATACAGTCAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTGGGGGCATATAACTTCTGTTGCAGAGATATTATCCCCTTGGTTTTGGAATTCCTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGCCTTATATTGTCTACTTGGAAATCACAGTGGTGTGTGCCTGGCAAACCTTCATGATTGGGACTGTATTGTGCAGACCTGGCCAGCACTTGTTTCTTCAGGGCTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGAGACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTGGATTTTACAATTCCAAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTTAGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAAGATAAGAATGCTGATGCCCTAAGGAACTATGAATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGGAATCGGGCTTCTGTCTCTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTTGAAAATCTCAACCATGATGCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGCAGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGAAAGATGGCTATCTCAGCT
59.04
AGGTTCCACAGCAACTCCTT
59.15
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGAAAGATGGCTATCTCAGCT
59.04
AAGGTTCCACAGCAACTCCT
59.15
142
PCR template sequence:
GCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGCAGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATCAATGATGATGTATTGAATGAAGAAGAGCTAGACAAGGAGTTGCTGTGGAACCTTCAGCTTCTGTCTGAGATTACTCGTGTAGACGGGAAGAAGTTACTTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
24
Interacting miRNAs (#binding sites)
mmu-miR-3470b(2), mmu-let-7a-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-135a-5p(1), mmu-let-7c-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-216a-5p(1), mmu-miR-181b-5p(1), mmu-let-7d-5p(1), mmu-miR-425-5p(1), mmu-miR-362-5p(1), mmu-miR-98-5p(1), mmu-miR-190b-5p(1), mmu-miR-3065-5p(1), mmu-miR-544-5p(1), mmu-miR-296-3p(1), mmu-miR-181d-5p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTTATGAAATCAATGATGATGTATT
siRNA sets
ATACATCATCATTGATTTC, TACATCATCATTGATTTCA, AATACATCATCATTGATTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPSME4_1710_ORF_1
0.8937
0.017898001
0.999999984
2985
ORF sequence:
ATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGTACACAGCTGCTTGATTGGTTCAGGGAACCTTCTACCTCCATTGAAAGGAGAGGCAGTTACTAACTTGGTACCAAGTATGGTCTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAGAGAAAACTACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATACCAAATCACTGTTTCTCATCATAAAGATCATTGGAGACCTTCTGCACTTCCAAGGATCTCATAAACATGAATTTGACTCTCGATGGAAAAGCTTTAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGCAACATATCAGAGCATTACTAATTGACAGAGTGATGCTGCAGCATGAGTTGCGAACACTAACTGTTGAGGGTTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGCTCATACAGTCAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTGGGGGCATATAACTTCTGTTGCAGAGATATTATCCCCTTGGTTTTGGAATTCCTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGCCTTATATTGTCTACTTGGAAATCACAGTGGTGTGTGCCTGGCAAACCTTCATGATTGGGACTGTATTGTGCAGACCTGGCCAGCACTTGTTTCTTCAGGGCTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGAGACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTGGATTTTACAATTCCAAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTTAGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAAGATAAGAATGCTGATGCCCTAAGGAACTATGAATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGGAATCGGGCTTCTGTCTCTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTTGAAAATCTCAACCATGATGCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGCAGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATCAATGATGATGTATTGAATGAAGAAGAGCTAGACAAGGAGTTGCTGTGGAACCTTCAGCTTCTGTCTGAGATTACTCGTGTAGACGGGAAGAAGTTACTTCTGTATAGGGAGCAGCTTGTAAAGATTCTCCAGCGAACCTTACACCTGACCTGTAAGCAGGGCTACACGCTGTCTTGCAACCTTCTGCATCATCTTCTTCGTTCTACCACACTAATCTACCCTACAGAGTACTGCAGTGTTCCGGGGGGCTTTAACAAGCCGCCTTCTGAATACTTCCCTGTCAAGGACTGGGGTAAGCCTGGAGATTTGTGGAATCTGGGGATCCAGTGGCATGTTCCTTCTTCAGAAGAAGTCTCTTTTGCCTTCTATTTGTTAGACTCCTTTCTTCAGCCTGAGCTTATCAAACTCCAGTGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGTACACAGCTGCTTGATTGGTTCAGGGAACCTTCTACCTCCATTGAAAGGAGAGGCAGTTACTAACTTGGTACCAAGTATGGTCTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAGAGAAAACTACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATACCAAATCACTGTTTCTCATCATAAAGATCATTGGAGACCTTCTGCACTTCCAAGGATCTCATAAACATGAATTTGACTCTCGATGGAAAAGCTTTAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGCAACATATCAGAGCATTACTAATTGACAGAGTGATGCTGCAGCATGAGTTGCGAACACTAACTGTTGAGGGTTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGCTCATACAGTCAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTGGGGGCATATAACTTCTGTTGCAGAGATATTATCCCCTTGGTTTTGGAATTCCTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGCCTTATATTGTCTACTTGGAAATCACAGTGGTGTGTGCCTGGCAAACCTTCATGATTGGGACTGTATTGTGCAGACCTGGCCAGCACTTGTTTCTTCAGGGCTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGAGACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTGGATTTTACAATTCCAAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTTAGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAAGATAAGAATGCTGATGCCCTAAGGAACTATGAATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGGAATCGGGCTTCTGTCTCTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTTGAAAATCTCAACCATGATGCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGCAGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATC
Peptide sequence:
MSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEINDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEI
IRESfinder result
IRESfinder score
0.686431076
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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