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mmu_circPrrc2b_334
Basic information of circular RNA
PanCircBase ID:
mmu_circPrrc2b_334
mm39_Chr. Position ID:
chr2|32106057|32106769|+
Gene name:
Prrc2b
Transcript:
ENSMUST00000036691.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
16,17
Exon/intron offsets:
0,566
Genomic length:
712
Exon/intron sizes in nt:
1,88,146
Flanking exon/introns:
chr2:32102303-32106057|chr2:32106769-32107208
Splice length:
334
Other database IDs:
circBase: hsa_circ_0089224, CIRCpedia: HSA_CIRCpedia_112428
Human Ortholog IDs:
Mature spliced Sequence:
GTCTCCAGATGAGGCCTTGCCTGGAGGCCTTGGCAGCCATTCACCCTATGCCTTGGAGAGAACCACTCACGCCAGCTCTGACGGTCCTGAAACCCCCAGTAAGAAATCAGAGAGAGAGGTTTCTTTGCCTACCCAGAGGGCTAGTGAGCAGGAAGAGGCCCGGAAGCAGTTTGACCTGGGCTATGGAAATGCTCTCATTGACAACTGCGCCTCCAGCCCTGGGGAAGAGAATGAAGCAAGCTCTGTGGTGGGTGAAGGCTTCATTGAAGTCCTGACCAAGAAGCAGCGTCGCCTGCTGGAGGAGGAGAGAAGAAAAAAAGAACAGGCTGCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCCTGACCAAGAAGCAGC
59.96
GGGTTTCAGGACCGTCAGAG
60.04
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCTCTGTGGTGGGTGAAG
59.89
GCGTGAGTGGTTCTCTCCAA
59.97
169
PCR template sequence:
AGCAAGCTCTGTGGTGGGTGAAGGCTTCATTGAAGTCCTGACCAAGAAGCAGCGTCGCCTGCTGGAGGAGGAGAGAAGAAAAAAAGAACAGGCTGCTCAGGTCTCCAGATGAGGCCTTGCCTGGAGGCCTTGGCAGCCATTCACCCTATGCCTTGGAGAGAACCACTCACGCCAGCTCTGACGGTCCTGAAACCCCCAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-29b-1-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACAGGCTGCTCAGGTCTCCAGATGAG
siRNA sets
TCATCTGGAGACCTGAGCA, CATCTGGAGACCTGAGCAG, CTCATCTGGAGACCTGAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.543940427
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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