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mmu_circPrrc1_371
Basic information of circular RNA
PanCircBase ID:
mmu_circPrrc1_371
mm39_Chr. Position ID:
chr18|57504438|57514803|+
Gene name:
Prrc1
Transcript:
ENSMUST00000025490.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,3160,10261
Genomic length:
10365
Exon/intron sizes in nt:
10,31,64,104
Flanking exon/introns:
chr18:57498421-57504438|chr18:57514803-57517245
Splice length:
371
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGCGTAGCTGGGAACCCTATGGTGAAATCTGTCCTGGATAAGACCAAGCATTCTGTAGAAAGCATGATCACCACGCTGGATCCTGGCATGGCTCCATATATCAAATCTGGAGGTGAACTGGATATTGTCGTAACCTCAAATAAAGAAGTGAAAGTGGCTGCTGTCCGAGATGCCTTCCAGGAGGTCTTTGGCTTAGCTGTGGTTGTTGGGGAAGCTGGACAGTCCAATATCGCCCCTCAGCCAGTCGGGTATGCAGCTGGATTGAAGGGCGCCCAGGAACGCATCGACAGCCTGAGGAGGTCGGGAGCGATCCACGAGAAACAGACGGCGGTGTCGGTAGAAAACTTCATTGCTGAGCTGCTGCCTGACAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGCGATCCACGAGAAACA
60.11
GGATCCAGCGTGGTGATCAT
59.89
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCCACGAGAAACAGACGGC
60.39
GGATCCAGCGTGGTGATCAT
59.89
145
PCR template sequence:
CCCAGGAACGCATCGACAGCCTGAGGAGGTCGGGAGCGATCCACGAGAAACAGACGGCGGTGTCGGTAGAAAACTTCATTGCTGAGCTGCTGCCTGACAAGGCGTAGCTGGGAACCCTATGGTGAAATCTGTCCTGGATAAGACCAAGCATTCTGTAGAAAGCATGATCACCACGCTGGATCCTGGCATGGCTCCATATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-680(1), mmu-miR-1198-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTGCCTGACAAGGCGTAGCTGGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPRRC1_371_ORF_1
1.131
0.403422138
0.74839857
360
ORF sequence:
ATGGTGAAATCTGTCCTGGATAAGACCAAGCATTCTGTAGAAAGCATGATCACCACGCTGGATCCTGGCATGGCTCCATATATCAAATCTGGAGGTGAACTGGATATTGTCGTAACCTCAAATAAAGAAGTGAAAGTGGCTGCTGTCCGAGATGCCTTCCAGGAGGTCTTTGGCTTAGCTGTGGTTGTTGGGGAAGCTGGACAGTCCAATATCGCCCCTCAGCCAGTCGGGTATGCAGCTGGATTGAAGGGCGCCCAGGAACGCATCGACAGCCTGAGGAGGTCGGGAGCGATCCACGAGAAACAGACGGCGGTGTCGGTAGAAAACTTCATTGCTGAGCTGCTGCCTGACAAGGCGTAG
Peptide sequence:
MVKSVLDKTKHSVESMITTLDPGMAPYIKSGGELDIVVTSNKEVKVAAVRDAFQEVFGLAVVVGEAGQSNIAPQPVGYAAGLKGAQERIDSLRRSGAIHEKQTAVSVENFIAELLPDKA
IRESfinder result
IRESfinder score
0.332961983
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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