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mmu_circPrr14l_521
Basic information of circular RNA
PanCircBase ID:
mmu_circPrr14l_521
mm39_Chr. Position ID:
chr5|33001387|33001908|-
Gene name:
Prr14l
Transcript:
ENSMUST00000120129.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
521
Exon/intron sizes in nt:
521
Flanking exon/introns:
chr5:32993032-33001387|chr5:33001908-33009225
Splice length:
521
Other database IDs:
CIRCpedia: MMU_CIRCpedia_16676
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_36452
Human Ortholog IDs:
Mature spliced Sequence:
GGTAAACCATTGATGCAAAAGACTTGACTCCATAATCTCCATCTGCCCTAATGCTGTCATCTGGAGTAGAGACCCAGCCAGTTCCACTTGATTCCTCCATGTCTGCTGTGGTACAGGAATTGTACTCGGAACTCTCAGTAAGTGTCTCCAAAGAGCTTCATGCTGAGCAGGAGCCAAGTAAGATTGCAGATGTAAAGCCTGGAGCCTCACGCTCTCCTCTAAGTCATGGCAGGGCCTCACCCCTGGGGTTGCAGAGGACTCATGCAGAAGGTTGTCGAGAAGAAAGCTCTGAGACCTTGGATTGTCGGGGCAAGAATGGGTGGTGCGGACTAGTGGATCCTACAAGAGATCCTATGGCTTATGAGATCTTGGATAGGGAAGAGAGACCCAAGAGCATGGAGCCAAAGGTCTTCAGAGATCAAGGAGACCAGGCACAAGTTTTCAGAGACCCATGTGAGGGAGCAAAGGAAGACCCGTGCCAACATTCCACAGCTACTAAAGAAAAGATCTGCCCCTGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGGAGCAAAGGAAGACCC
60.03
GGCTGGGTCTCTACTCCAGA
60.03
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGGAGCAAAGGAAGACCC
60.03
TGGAACTGGCTGGGTCTCTA
59.88
152
PCR template sequence:
AGGAGACCAGGCACAAGTTTTCAGAGACCCATGTGAGGGAGCAAAGGAAGACCCGTGCCAACATTCCACAGCTACTAAAGAAAAGATCTGCCCCTGTCAGGGTAAACCATTGATGCAAAAGACTTGACTCCATAATCTCCATCTGCCCTAATGCTGTCATCTGGAGTAGAGACCCAGCCAGTTCCACTTGATTCCTCCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-206-3p(1), mmu-miR-127-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTGCCCCTGTCAGGGTAAACCATTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPRR14L_521_ORF_1
0.8665
0.109903488
0.595555578
498
ORF sequence:
ATGCTGTCATCTGGAGTAGAGACCCAGCCAGTTCCACTTGATTCCTCCATGTCTGCTGTGGTACAGGAATTGTACTCGGAACTCTCAGTAAGTGTCTCCAAAGAGCTTCATGCTGAGCAGGAGCCAAGTAAGATTGCAGATGTAAAGCCTGGAGCCTCACGCTCTCCTCTAAGTCATGGCAGGGCCTCACCCCTGGGGTTGCAGAGGACTCATGCAGAAGGTTGTCGAGAAGAAAGCTCTGAGACCTTGGATTGTCGGGGCAAGAATGGGTGGTGCGGACTAGTGGATCCTACAAGAGATCCTATGGCTTATGAGATCTTGGATAGGGAAGAGAGACCCAAGAGCATGGAGCCAAAGGTCTTCAGAGATCAAGGAGACCAGGCACAAGTTTTCAGAGACCCATGTGAGGGAGCAAAGGAAGACCCGTGCCAACATTCCACAGCTACTAAAGAAAAGATCTGCCCCTGTCAGGGTAAACCATTGATGCAAAAGACTTGA
Peptide sequence:
MLSSGVETQPVPLDSSMSAVVQELYSELSVSVSKELHAEQEPSKIADVKPGASRSPLSHGRASPLGLQRTHAEGCREESSETLDCRGKNGWCGLVDPTRDPMAYEILDREERPKSMEPKVFRDQGDQAQVFRDPCEGAKEDPCQHSTATKEKICPCQGKPLMQKT
IRESfinder result
IRESfinder score
0.420127013
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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