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mmu_circPrpsap2_345
Basic information of circular RNA
PanCircBase ID:
mmu_circPrpsap2_345
mm39_Chr. Position ID:
chr11|61631771|61639910|-
Gene name:
Prpsap2
Transcript:
ENSMUST00000168115.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,6,5
Exon/intron offsets:
0,3999,7966
Genomic length:
8139
Exon/intron sizes in nt:
5,61,16,173
Flanking exon/introns:
chr11:61627890-61631771|chr11:61639910-61643692
Splice length:
345
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGACGTGAACACCACCATCATGGAGCTGCTGATCATGGTATATGCATGTAAGACCTCATGTGCCAAGAGCATCATCGGAGTGATCCCCTACTTTCCTTATAGCAAGCAATGCAAAATGAGAAAAAGGGGCTCCATTGTCTCAAAATTGCTGGCTTCTATGATGTGCAAAGCTGGTCTGACTCATCTCATCACTATGGATTTACACCAGAAGGAAATCCAGGGCTTCTTCAATATTCCAGTGGATAATTTAAGAGCCTCCCCATTCCTGTTACAATATATTCAAGAAGAGATCCCAGATTATAGGAATGCAGTCATCGTGGCCAAGTCTCCAGCCTCAGCCAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGCCTCCCCATTCCTGTT
60.55
ACTCCGATGATGCTCTTGGC
60.18
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGAGCCTCCCCATTCCTGT
60.55
ACTCCGATGATGCTCTTGGC
60.18
177
PCR template sequence:
ATTTAAGAGCCTCCCCATTCCTGTTACAATATATTCAAGAAGAGATCCCAGATTATAGGAATGCAGTCATCGTGGCCAAGTCTCCAGCCTCAGCCAAGAGGGACGTGAACACCACCATCATGGAGCTGCTGATCATGGTATATGCATGTAAGACCTCATGTGCCAAGAGCATCATCGGAGTGATCCCCTACTTTCCTTAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-143-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTCAGCCAAGAGGGACGTGAACACC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPRPSAP2_345_ORF_1
1.126
0.210985816
0.925881027
669
ORF sequence:
ATGGAGCTGCTGATCATGGTATATGCATGTAAGACCTCATGTGCCAAGAGCATCATCGGAGTGATCCCCTACTTTCCTTATAGCAAGCAATGCAAAATGAGAAAAAGGGGCTCCATTGTCTCAAAATTGCTGGCTTCTATGATGTGCAAAGCTGGTCTGACTCATCTCATCACTATGGATTTACACCAGAAGGAAATCCAGGGCTTCTTCAATATTCCAGTGGATAATTTAAGAGCCTCCCCATTCCTGTTACAATATATTCAAGAAGAGATCCCAGATTATAGGAATGCAGTCATCGTGGCCAAGTCTCCAGCCTCAGCCAAGAGGGACGTGAACACCACCATCATGGAGCTGCTGATCATGGTATATGCATGTAAGACCTCATGTGCCAAGAGCATCATCGGAGTGATCCCCTACTTTCCTTATAGCAAGCAATGCAAAATGAGAAAAAGGGGCTCCATTGTCTCAAAATTGCTGGCTTCTATGATGTGCAAAGCTGGTCTGACTCATCTCATCACTATGGATTTACACCAGAAGGAAATCCAGGGCTTCTTCAATATTCCAGTGGATAATTTAAGAGCCTCCCCATTCCTGTTACAATATATTCAAGAAGAGATCCCAGATTATAGGAATGCAGTCATCGTGGCCAAGTCTCCAGCCTCAGCCAAG
Peptide sequence:
MELLIMVYACKTSCAKSIIGVIPYFPYSKQCKMRKRGSIVSKLLASMMCKAGLTHLITMDLHQKEIQGFFNIPVDNLRASPFLLQYIQEEIPDYRNAVIVAKSPASAKRDVNTTIMELLIMVYACKTSCAKSIIGVIPYFPYSKQCKMRKRGSIVSKLLASMMCKAGLTHLITMDLHQKEIQGFFNIPVDNLRASPFLLQYIQEEIPDYRNAVIVAKSPASAK
IRESfinder result
IRESfinder score
0.644896543
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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