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mmu_circPrmt3_826
Basic information of circular RNA
PanCircBase ID:
mmu_circPrmt3_826
mm39_Chr. Position ID:
chr7|49430056|49456374|+
Gene name:
Prmt3
Transcript:
ENSMUST00000032715.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
3,4,5,6,7,8,9,10
Exon/intron offsets:
0,812,1633,5758,6924,11664,17997,26218
Genomic length:
26318
Exon/intron sizes in nt:
83,50,10,31,60,14,26,60,00,000
Flanking exon/introns:
chr7:49428461-49430056|chr7:49456374-49467779
Splice length:
826
Other database IDs:
circAtlas: hsa-PRMT3_0028
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTTCGCCTCTGCTGAGGAAACGTTTTCACACTGTAAGTTGGAGCATCAATTTAATATTGATAGTATGGTCCATAAACATGGACTTGAATTTTATGGATACATTAAACTAATAAATTTTATTAGACTTAAGAATCCTACGGTTGAATATATGAATTCCATATATAACCCTGTGCCTTGGGAAAAAGATGAGTACCTGAAGCCAGTGTTGGAAGATGACCTTTTGCTTCAGTTTGATGTAGAAGATCTTTATGAGCCTGTGTCTACTCCATTCTCATACCCCAATGGACTCAGTGAAAGCGCATCTGTTGTTGAGAAGTTGAAGCACATGGAAGCGCGGGCGCTGTCTGCTGAGGCTGCCTTGGCTCGGGCGCGTGAGGATCTGCAGAAGATGAAACAATTTGCTCAGGACTTTGTGATGAACGTAGATGTCAGAACCTGCTCCTCCACGACGACCATTGCAGACCTGCAGGAGGATGAGGATGGCGTCTACTTCAGCTCCTATGGGCATTATGGGATACATGAAGAAATGCTAAAGGACAAAGTACGCACAGAAAGTTACAGAGATTTCATATACCAAAATCCACACATCTTCAAAGATAAGGTTGTTCTGGATGTTGGGTGTGGAACTGGAATTCTCTCCATGTTTGCTGCCAAAGTTGGGGCAAAGAAAGTGATTGCAGTCGACCAGTCTGAAATCCTCTACCAGGCTATGGATATCATAAGACTAAATAAACTTGAAGATACCATTGTACTAATTAAAGGAAAGATTGAAGAAGTGAGTCTTCCTGTGGAAAAGGTGGATGTTATTATATCAGAGTGGATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCTGTGGAAAAGGTGGATGT
59.22
TCCATAAAATTCAAGTCCATGTTT
55.24
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTCCTGTGGAAAAGGTGGA
58.87
TCCATAAAATTCAAGTCCATGTTT
55.24
143
PCR template sequence:
ACTAAATAAACTTGAAGATACCATTGTACTAATTAAAGGAAAGATTGAAGAAGTGAGTCTTCCTGTGGAAAAGGTGGATGTTATTATATCAGAGTGGATGGTTGTTCGCCTCTGCTGAGGAAACGTTTTCACACTGTAAGTTGGAGCATCAATTTAATATTGATAGTATGGTCCATAAACATGGACTTGAATTTTATGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-124-3p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-185-5p(1), mmu-miR-301b-3p(1), mmu-miR-218-5p(1), mmu-miR-301a-3p(1), mmu-miR-1187(1), mmu-miR-350-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCAGAGTGGATGGTTGTTCGCCTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPRMT3_826_ORF_1
1.0382
0.136498235
0.9655673
849
ORF sequence:
ATGTTATTATATCAGAGTGGATGGTTGTTCGCCTCTGCTGAGGAAACGTTTTCACACTGTAAGTTGGAGCATCAATTTAATATTGATAGTATGGTCCATAAACATGGACTTGAATTTTATGGATACATTAAACTAATAAATTTTATTAGACTTAAGAATCCTACGGTTGAATATATGAATTCCATATATAACCCTGTGCCTTGGGAAAAAGATGAGTACCTGAAGCCAGTGTTGGAAGATGACCTTTTGCTTCAGTTTGATGTAGAAGATCTTTATGAGCCTGTGTCTACTCCATTCTCATACCCCAATGGACTCAGTGAAAGCGCATCTGTTGTTGAGAAGTTGAAGCACATGGAAGCGCGGGCGCTGTCTGCTGAGGCTGCCTTGGCTCGGGCGCGTGAGGATCTGCAGAAGATGAAACAATTTGCTCAGGACTTTGTGATGAACGTAGATGTCAGAACCTGCTCCTCCACGACGACCATTGCAGACCTGCAGGAGGATGAGGATGGCGTCTACTTCAGCTCCTATGGGCATTATGGGATACATGAAGAAATGCTAAAGGACAAAGTACGCACAGAAAGTTACAGAGATTTCATATACCAAAATCCACACATCTTCAAAGATAAGGTTGTTCTGGATGTTGGGTGTGGAACTGGAATTCTCTCCATGTTTGCTGCCAAAGTTGGGGCAAAGAAAGTGATTGCAGTCGACCAGTCTGAAATCCTCTACCAGGCTATGGATATCATAAGACTAAATAAACTTGAAGATACCATTGTACTAATTAAAGGAAAGATTGAAGAAGTGAGTCTTCCTGTGGAAAAGGTGGATGTTATTATATCAGAGTGGATG
Peptide sequence:
MLLYQSGWLFASAEETFSHCKLEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKDEYLKPVLEDDLLLQFDVEDLYEPVSTPFSYPNGLSESASVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMNVDVRTCSSTTTIADLQEDEDGVYFSSYGHYGIHEEMLKDKVRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKVGAKKVIAVDQSEILYQAMDIIRLNKLEDTIVLIKGKIEEVSLPVEKVDVIISEWM
IRESfinder result
IRESfinder score
0.524178268
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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