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mmu_circPpp6r3_864
Basic information of circular RNA
PanCircBase ID:
mmu_circPpp6r3_864
mm39_Chr. Position ID:
chr19|3542365|3568382|-
Gene name:
Ppp6r3
Transcript:
ENSMUST00000025846.16
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
11,10,9,8,7,6,5
Exon/intron offsets:
0,1520,4156,14876,19563,22288,25879
Genomic length:
26017
Exon/intron sizes in nt:
150,153,130,114,113,66,138
Flanking exon/introns:
chr19:3540838-3542365|chr19:3568382-3571733
Splice length:
864
Other database IDs:
CIRCpedia: MMU_CIRCpedia_17556
circAtlas: hsa-PPP6R3_0178
Human Ortholog IDs:
Mature spliced Sequence:
ATTGTGGATTTCTTGAAAAAGAAGCGTGATTTTGTGGATCTCATTATAAAGCACATAGGAACTTCTGCTATCATGGATTTATTGCTCAGGCTCTTGACATGTATTGAGCCTCCACAACCCAGGCAAGATGTTCTGAATTGGCTAAATGAGGAGAGGATTATCCAGAGGCTTGTGGAAATAGTTCACCCATCGCAAGAAGAAGATCGGCATTCAAATGCCTCACAATCACTTTGTGAAATTGTTCGCCTGAGCAGAGACCAGATGTTACAAGTTCAGAACAGTACAGAGCCAGACCCCCTGCTTGCCACTCTAGAAAAGCAAGAAATTATTGAGCAGCTTCTGTCAAATATTTTTCACAAGGAGAAGAATGAATCGGCCATAGTCAGTGCAATTCAGATTTTGCTGACTTTGCTTGAGACTCGACGGCCAACGTTTGAAGGCCATATAGAAATCTGCCCCCCGGGCATGAGTCATTCAGCTTGTTCAGTTAACAAGAGTGTTCTTGAAGCCATCCGAGGAAGACTTGGATCTTTCCATGAACTCCTACTCGAGCCACCCAAGAAAAGTGTGATGAAGACTACATGGGGTATATTGGACCCACCTGTGGGGAATACTCGATTAAATGTCATTAGGTTGATATCCAGCCTGCTCCAAACCAATACCAGCAGCATAAATGGAGACCTGATGGAACTGAACAGCATTGGCGTCATATTGGATATGTTCTTCAAGTACACATGGAATAACTTTCTACACACGCAAGTGGAAATTTGCATTGCTCTCATTCTGGCCAGTCCTTTTGAGAATGCAGAGAATGGCACAATTACTGATCAAGACTCCACTGGTGACAATTTGTTATTGAAGCAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTCTCATTCTGGCCAGTC
59.75
AGCAGAAGTTCCTATGTGCT
56.54
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCATTGCTCTCATTCTGGCC
59.62
AGCAGAAGTTCCTATGTGCT
56.54
164
PCR template sequence:
AATTTGCATTGCTCTCATTCTGGCCAGTCCTTTTGAGAATGCAGAGAATGGCACAATTACTGATCAAGACTCCACTGGTGACAATTTGTTATTGAAGCATATTGTGGATTTCTTGAAAAAGAAGCGTGATTTTGTGGATCTCATTATAAAGCACATAGGAACTTCTGCTATCATGGATTTATTGCTCAGGCTCTTGACAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-miR-92a-3p(1), mmu-miR-335-5p(1), mmu-miR-25-3p(1), mmu-miR-138-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-802-5p(1), mmu-miR-544-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTATTGAAGCATATTGTGGATTTCT
siRNA sets
AAATCCACAATATGCTTCA, GAAATCCACAATATGCTTC, AATCCACAATATGCTTCAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPP6R3_864_ORF_1
1.0256
0.092449826
0.999870537
1656
ORF sequence:
ATGGATTTATTGCTCAGGCTCTTGACATGTATTGAGCCTCCACAACCCAGGCAAGATGTTCTGAATTGGCTAAATGAGGAGAGGATTATCCAGAGGCTTGTGGAAATAGTTCACCCATCGCAAGAAGAAGATCGGCATTCAAATGCCTCACAATCACTTTGTGAAATTGTTCGCCTGAGCAGAGACCAGATGTTACAAGTTCAGAACAGTACAGAGCCAGACCCCCTGCTTGCCACTCTAGAAAAGCAAGAAATTATTGAGCAGCTTCTGTCAAATATTTTTCACAAGGAGAAGAATGAATCGGCCATAGTCAGTGCAATTCAGATTTTGCTGACTTTGCTTGAGACTCGACGGCCAACGTTTGAAGGCCATATAGAAATCTGCCCCCCGGGCATGAGTCATTCAGCTTGTTCAGTTAACAAGAGTGTTCTTGAAGCCATCCGAGGAAGACTTGGATCTTTCCATGAACTCCTACTCGAGCCACCCAAGAAAAGTGTGATGAAGACTACATGGGGTATATTGGACCCACCTGTGGGGAATACTCGATTAAATGTCATTAGGTTGATATCCAGCCTGCTCCAAACCAATACCAGCAGCATAAATGGAGACCTGATGGAACTGAACAGCATTGGCGTCATATTGGATATGTTCTTCAAGTACACATGGAATAACTTTCTACACACGCAAGTGGAAATTTGCATTGCTCTCATTCTGGCCAGTCCTTTTGAGAATGCAGAGAATGGCACAATTACTGATCAAGACTCCACTGGTGACAATTTGTTATTGAAGCATATTGTGGATTTCTTGAAAAAGAAGCGTGATTTTGTGGATCTCATTATAAAGCACATAGGAACTTCTGCTATCATGGATTTATTGCTCAGGCTCTTGACATGTATTGAGCCTCCACAACCCAGGCAAGATGTTCTGAATTGGCTAAATGAGGAGAGGATTATCCAGAGGCTTGTGGAAATAGTTCACCCATCGCAAGAAGAAGATCGGCATTCAAATGCCTCACAATCACTTTGTGAAATTGTTCGCCTGAGCAGAGACCAGATGTTACAAGTTCAGAACAGTACAGAGCCAGACCCCCTGCTTGCCACTCTAGAAAAGCAAGAAATTATTGAGCAGCTTCTGTCAAATATTTTTCACAAGGAGAAGAATGAATCGGCCATAGTCAGTGCAATTCAGATTTTGCTGACTTTGCTTGAGACTCGACGGCCAACGTTTGAAGGCCATATAGAAATCTGCCCCCCGGGCATGAGTCATTCAGCTTGTTCAGTTAACAAGAGTGTTCTTGAAGCCATCCGAGGAAGACTTGGATCTTTCCATGAACTCCTACTCGAGCCACCCAAGAAAAGTGTGATGAAGACTACATGGGGTATATTGGACCCACCTGTGGGGAATACTCGATTAAATGTCATTAGGTTGATATCCAGCCTGCTCCAAACCAATACCAGCAGCATAAATGGAGACCTGATGGAACTGAACAGCATTGGCGTCATATTGGATATGTTCTTCAAGTACACATGGAATAACTTTCTACACACGCAAGTGGAAATTTGCATTGCTCTCATTCTGGCCAGTCCTTTTGAGAATGCAGAGAATGGCACAATTACTGATCAAGACTCCACTGGTGACAATTTGTTATTGAAGCAT
Peptide sequence:
MDLLLRLLTCIEPPQPRQDVLNWLNEERIIQRLVEIVHPSQEEDRHSNASQSLCEIVRLSRDQMLQVQNSTEPDPLLATLEKQEIIEQLLSNIFHKEKNESAIVSAIQILLTLLETRRPTFEGHIEICPPGMSHSACSVNKSVLEAIRGRLGSFHELLLEPPKKSVMKTTWGILDPPVGNTRLNVIRLISSLLQTNTSSINGDLMELNSIGVILDMFFKYTWNNFLHTQVEICIALILASPFENAENGTITDQDSTGDNLLLKHIVDFLKKKRDFVDLIIKHIGTSAIMDLLLRLLTCIEPPQPRQDVLNWLNEERIIQRLVEIVHPSQEEDRHSNASQSLCEIVRLSRDQMLQVQNSTEPDPLLATLEKQEIIEQLLSNIFHKEKNESAIVSAIQILLTLLETRRPTFEGHIEICPPGMSHSACSVNKSVLEAIRGRLGSFHELLLEPPKKSVMKTTWGILDPPVGNTRLNVIRLISSLLQTNTSSINGDLMELNSIGVILDMFFKYTWNNFLHTQVEICIALILASPFENAENGTITDQDSTGDNLLLKH
IRESfinder result
IRESfinder score
0.692776398
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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