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mmu_circPpp6r1_111
Basic information of circular RNA
PanCircBase ID:
mmu_circPpp6r1_111
mm39_Chr. Position ID:
chr7|4635487|4635598|-
Gene name:
Ppp6r1
Transcript:
ENSMUST00000064099.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
22
Exon/intron offsets:
0
Genomic length:
111
Exon/intron sizes in nt:
111
Flanking exon/introns:
chr7:4635396-4635487|chr7:4635598-4636057
Splice length:
111
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGCAACCAGAGACCCCTCTACCTCTGTCCCAGACTTCAAGGCCCACCAGTCTCCTCAGACCATGGAGGGGAAAAGGAGCCCAGAACATTTGGGGCTTCCCCAAAGCCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAAAAGGAGCCCAGAACA
59.89
GAGACTGGTGGGCCTTGAAG
60.32
97
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGCCAGAGTGCAACCAGA
59.82
GAGACTGGTGGGCCTTGAAG
60.32
63
PCR template sequence:
AGACCATGGAGGGGAAAAGGAGCCCAGAACATTTGGGGCTTCCCCAAAGCCAGAGTGCAACCAGAGACCCCTCTACCTCTGTCCCAGACTTCAAGGCCCACCAGTCTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCAAAGCCAGAGTGCAACCAGAGAC
siRNA sets
GTCTCTGGTTGCACTCTGG,GTTGCACTCTGGCTTTGGG,TCTCTGGTTGCACTCTGGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPP6R1_111_ORF_1
0.6436
0.05106839
0.0632525
159
ORF sequence:
ATGGAGGGGAAAAGGAGCCCAGAACATTTGGGGCTTCCCCAAAGCCAGAGTGCAACCAGAGACCCCTCTACCTCTGTCCCAGACTTCAAGGCCCACCAGTCTCCTCAGACCATGGAGGGGAAAAGGAGCCCAGAACATTTGGGGCTTCCCCAAAGCCAG
Peptide sequence:
MEGKRSPEHLGLPQSQSATRDPSTSVPDFKAHQSPQTMEGKRSPEHLGLPQSQ
IRESfinder result
IRESfinder score
0.72655465
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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