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mmu_circPpp1r21_1619
Basic information of circular RNA
PanCircBase ID:
mmu_circPpp1r21_1619
mm39_Chr. Position ID:
chr17|88862277|88891409|+
Gene name:
Ppp1r21
Transcript:
ENSMUST00000038551.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
8,9,10,11,12,13,14,15,16,17,18,19,20,21
Exon/intron offsets:
0,732,3739,3927,7259,10791,14230,17753,20342,22288,23932,25585,27565,29003
Genomic length:
29132
Exon/intron sizes in nt:
5,31,50,10,28,91,37,93,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr17:88858184-88862277|chr17:88891409-88895111
Splice length:
1619
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGTGTCATCTGTACAACGCTCTCAATGTTCCCCTGCACAATAGGCGACACCAGCTGAAGATGCGAGATATAGCTGGGCAGGCCCTGGCTTTTGTTCAGGATCTTGTACCGGCTCTTCTGAACTTTCACACCTATACAGAACAGAGGATTCAAATTTTCCCTGTTGATTCTGCCATAGACACTATATCACCATTGAATCAGAAGTTCTCCCAATACCTTCATGAAAACGCATCGTATGTCCGCCCCCTTGAGGAAGGAATGCTGCACTTATTCGAGAGCATTACAGAGGATACTGTGACTGTCCTGGAGACAACTGTGAAATTGAAAATGTTTTCAGATCATCTAACTTCCTACGTACGCTTCCTGAGGAAGATCCTTCCTTATCAGTTAAAAAGTTTAGAAGAAGAGTGTGAATCTTCCCTCTGCACACCTGCACTGAGAGCCAGGAACCTGGAGCTGTCCCAGGACATGAAGACGATGACAGCCGTGTTTGAGAAGCTGCAGACGTATGTCACTCTCCTCGCCTTGCCAAGTACAGAGCCAGATGGACTCCTTCGAACAAACTACACTTCCGTCTTAACAAATGTTGGTGCTGCTCTGCATGGATTTCATGATGTCATGAAAGATATTTCCAAACACTATAGTCAGAAGGCTTCCATAGAGCATGAAATTCCAACAGCAACACAGAAGCTAGTAACGACTAACGACTGCATCTTGTCCTCAGCGGTGACGTTAACAAACGGAGCGGGCAAGATTGCGTCTTTCTTCGGCAACAACGTGGACTACTTCATTGCTTCACTGAGCTATGGGCCCAAGACAGCCAGCGGATTCATTAGTCCCCTTTCGGCTGAGTGCATGCTGCAGTACAAGAAGAAGGCTGCTGCCTATATGAAGTCTTTGAGAACGCCCCTCGCAGAGTCTGTGCCTTACGGGGAAGCTGTGGCCAACCGCCGGGTTCTCCTCAGCTCTACAGAGAGTCGGGAAGGCCTTGCACAGCAGGTTCAGCAGAGTTTGGAAAAGATTTCTAAGCTGGAGCAGGAGAAAGAACATTGGATGCTAGAAGCACAGTTAGCCAAAATCAAGCTGGAGAAAGAAAACCAACGGATTGCAGACAGGCTGCGGGGCACCACCAGTGCCCAGCTGCCTGGGCTGGCCCAGGAGAATGCGACTGTGCCCATTGCTTCCAGCCAGGAGGAGGCTGCAGCCAAGGTCCTGACAGAGCCTGTGCAGAGCACCAGTCTGGTTGGGATGTTAACCAGGACGCCAGACAGTGAGGCTCCGGATGTTGAGTCCCGTGAAGACCTAATTAAGAGTCACTACATGGCCAGGATAGCGGAGCTCACATCTCAGCTGCAGCTGGCTGACAGTAAGTCAGTGCACTTCTACGCCGAGTGCCGTGCGCTCTCTAAGAGACTAGCCCTGGCGGAGAAGTCGAAGGAGACACTGACAGAGGAGATGAGGCTAGCCAGTCAGAACATCAGCAGGCTTCAGGACGAGCTGATGACCACCAAGCGGAGCTACGAGGATCAGCTGAGCATGATGAGCGACCACCTATGCAGCATGAACGAGACCCTGTCCAAACAGAGGGAGGAGATTGACACGCTCAAGATGGCCAGTAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCATGAACGAGACCCTGT
60.04
CCTGCCCAGCTATATCTCGC
60.11
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCATGAACGAGACCCTGT
60.04
GCCTGCCCAGCTATATCTCG
60.11
146
PCR template sequence:
CGAGGATCAGCTGAGCATGATGAGCGACCACCTATGCAGCATGAACGAGACCCTGTCCAAACAGAGGGAGGAGATTGACACGCTCAAGATGGCCAGTAAGAGTGTCATCTGTACAACGCTCTCAATGTTCCCCTGCACAATAGGCGACACCAGCTGAAGATGCGAGATATAGCTGGGCAGGCCCTGGCTTTTGTTCAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-138-5p(3), mmu-miR-155-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-196a-5p(1), mmu-miR-24-3p(1), mmu-miR-149-5p(1), mmu-miR-676-3p(1), mmu-miR-182-5p(1), mmu-miR-107-3p(1), mmu-miR-484(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGGCCAGTAAGAGTGTCATCTGTA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPP1R21_1619_ORF_1
1.1574
0.312023988
0.999943325
1617
ORF sequence:
ATGCGAGATATAGCTGGGCAGGCCCTGGCTTTTGTTCAGGATCTTGTACCGGCTCTTCTGAACTTTCACACCTATACAGAACAGAGGATTCAAATTTTCCCTGTTGATTCTGCCATAGACACTATATCACCATTGAATCAGAAGTTCTCCCAATACCTTCATGAAAACGCATCGTATGTCCGCCCCCTTGAGGAAGGAATGCTGCACTTATTCGAGAGCATTACAGAGGATACTGTGACTGTCCTGGAGACAACTGTGAAATTGAAAATGTTTTCAGATCATCTAACTTCCTACGTACGCTTCCTGAGGAAGATCCTTCCTTATCAGTTAAAAAGTTTAGAAGAAGAGTGTGAATCTTCCCTCTGCACACCTGCACTGAGAGCCAGGAACCTGGAGCTGTCCCAGGACATGAAGACGATGACAGCCGTGTTTGAGAAGCTGCAGACGTATGTCACTCTCCTCGCCTTGCCAAGTACAGAGCCAGATGGACTCCTTCGAACAAACTACACTTCCGTCTTAACAAATGTTGGTGCTGCTCTGCATGGATTTCATGATGTCATGAAAGATATTTCCAAACACTATAGTCAGAAGGCTTCCATAGAGCATGAAATTCCAACAGCAACACAGAAGCTAGTAACGACTAACGACTGCATCTTGTCCTCAGCGGTGACGTTAACAAACGGAGCGGGCAAGATTGCGTCTTTCTTCGGCAACAACGTGGACTACTTCATTGCTTCACTGAGCTATGGGCCCAAGACAGCCAGCGGATTCATTAGTCCCCTTTCGGCTGAGTGCATGCTGCAGTACAAGAAGAAGGCTGCTGCCTATATGAAGTCTTTGAGAACGCCCCTCGCAGAGTCTGTGCCTTACGGGGAAGCTGTGGCCAACCGCCGGGTTCTCCTCAGCTCTACAGAGAGTCGGGAAGGCCTTGCACAGCAGGTTCAGCAGAGTTTGGAAAAGATTTCTAAGCTGGAGCAGGAGAAAGAACATTGGATGCTAGAAGCACAGTTAGCCAAAATCAAGCTGGAGAAAGAAAACCAACGGATTGCAGACAGGCTGCGGGGCACCACCAGTGCCCAGCTGCCTGGGCTGGCCCAGGAGAATGCGACTGTGCCCATTGCTTCCAGCCAGGAGGAGGCTGCAGCCAAGGTCCTGACAGAGCCTGTGCAGAGCACCAGTCTGGTTGGGATGTTAACCAGGACGCCAGACAGTGAGGCTCCGGATGTTGAGTCCCGTGAAGACCTAATTAAGAGTCACTACATGGCCAGGATAGCGGAGCTCACATCTCAGCTGCAGCTGGCTGACAGTAAGTCAGTGCACTTCTACGCCGAGTGCCGTGCGCTCTCTAAGAGACTAGCCCTGGCGGAGAAGTCGAAGGAGACACTGACAGAGGAGATGAGGCTAGCCAGTCAGAACATCAGCAGGCTTCAGGACGAGCTGATGACCACCAAGCGGAGCTACGAGGATCAGCTGAGCATGATGAGCGACCACCTATGCAGCATGAACGAGACCCTGTCCAAACAGAGGGAGGAGATTGACACGCTCAAGATGGCCAGTAAGAGTGTCATCTGTACAACGCTCTCAATGTTCCCCTGCACAATAGGCGACACCAGCTGA
Peptide sequence:
MRDIAGQALAFVQDLVPALLNFHTYTEQRIQIFPVDSAIDTISPLNQKFSQYLHENASYVRPLEEGMLHLFESITEDTVTVLETTVKLKMFSDHLTSYVRFLRKILPYQLKSLEEECESSLCTPALRARNLELSQDMKTMTAVFEKLQTYVTLLALPSTEPDGLLRTNYTSVLTNVGAALHGFHDVMKDISKHYSQKASIEHEIPTATQKLVTTNDCILSSAVTLTNGAGKIASFFGNNVDYFIASLSYGPKTASGFISPLSAECMLQYKKKAAAYMKSLRTPLAESVPYGEAVANRRVLLSSTESREGLAQQVQQSLEKISKLEQEKEHWMLEAQLAKIKLEKENQRIADRLRGTTSAQLPGLAQENATVPIASSQEEAAAKVLTEPVQSTSLVGMLTRTPDSEAPDVESREDLIKSHYMARIAELTSQLQLADSKSVHFYAECRALSKRLALAEKSKETLTEEMRLASQNISRLQDELMTTKRSYEDQLSMMSDHLCSMNETLSKQREEIDTLKMASKSVICTTLSMFPCTIGDTS
IRESfinder result
IRESfinder score
0.438574873
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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