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mmu_circPpp1r10_521
Basic information of circular RNA
PanCircBase ID:
mmu_circPpp1r10_521
mm39_Chr. Position ID:
chr17|36237865|36239453|+
Gene name:
Ppp1r10
Transcript:
ENSMUST00000087211.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
10,11,12,13
Exon/intron offsets:
0,936,1208,1429
Genomic length:
1588
Exon/intron sizes in nt:
1,13,10,11,48,159
Flanking exon/introns:
chr17:36237769-36237865|chr17:36239453-36239543
Splice length:
521
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGCCACAGCTGCTCCAGGTGACGCTGCCCCACCTGCAGAGAAGAAATACAAGCCTCTCAACACAGCCCCCAATACCACCAAAGAAATCAAAGTGAAGATCATCCCTCCACAGCCTATGGAGGGCCTGGGTTTTCTGGATGCTCTCAATTCAGCCCCTGTCCCAGGCATCAAAATTAAGAAGAAGAAGAAGGTACTATCGCCGACTGCTGCCAAGCCCAGCCCTTTTGAAGGGAAAACAAGCACCGAGCAGAGCACAGCCAAACCTTCCTCCCCAGAGCCTGCACCACCTGCTGAGCCCATGGACACGGACCGCCCTGGCACCCCTGTTCCCCCTGTTGAAGTTCCAGAGCTCATGGATGCAGCCTCTTCAGAGCCAGGAGCTCTGGATGCAAAGCCTGTGGACAGCCCTGGAGATCCCAACCAGCTGACTCGCAAGGGCAGAAAGAGGAAAACTGTGACTTGGCCTGAGGAGGGCAAGCTGAGAGAGTATTTCTACTTTGAACTGGACGAAACTGAGCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTGACTTGGCCTGAGGAG
60.04
ATTGGGGGCTGTGTTGAGAG
59.96
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTGACTTGGCCTGAGGAG
60.04
GGTATTGGGGGCTGTGTTGA
59.96
145
PCR template sequence:
CAGCTGACTCGCAAGGGCAGAAAGAGGAAAACTGTGACTTGGCCTGAGGAGGGCAAGCTGAGAGAGTATTTCTACTTTGAACTGGACGAAACTGAGCGAGTGCCACAGCTGCTCCAGGTGACGCTGCCCCACCTGCAGAGAAGAAATACAAGCCTCTCAACACAGCCCCCAATACCACCAAAGAAATCAAAGTGAAGATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-224-5p(1), mmu-miR-27a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAACTGAGCGAGTGCCACAGCTGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPP1R10_521_ORF_1
1.063
0.333237157
0.843851473
501
ORF sequence:
ATGGAGGGCCTGGGTTTTCTGGATGCTCTCAATTCAGCCCCTGTCCCAGGCATCAAAATTAAGAAGAAGAAGAAGGTACTATCGCCGACTGCTGCCAAGCCCAGCCCTTTTGAAGGGAAAACAAGCACCGAGCAGAGCACAGCCAAACCTTCCTCCCCAGAGCCTGCACCACCTGCTGAGCCCATGGACACGGACCGCCCTGGCACCCCTGTTCCCCCTGTTGAAGTTCCAGAGCTCATGGATGCAGCCTCTTCAGAGCCAGGAGCTCTGGATGCAAAGCCTGTGGACAGCCCTGGAGATCCCAACCAGCTGACTCGCAAGGGCAGAAAGAGGAAAACTGTGACTTGGCCTGAGGAGGGCAAGCTGAGAGAGTATTTCTACTTTGAACTGGACGAAACTGAGCGAGTGCCACAGCTGCTCCAGGTGACGCTGCCCCACCTGCAGAGAAGAAATACAAGCCTCTCAACACAGCCCCCAATACCACCAAAGAAATCAAAGTGA
Peptide sequence:
MEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEQSTAKPSSPEPAPPAEPMDTDRPGTPVPPVEVPELMDAASSEPGALDAKPVDSPGDPNQLTRKGRKRKTVTWPEEGKLREYFYFELDETERVPQLLQVTLPHLQRRNTSLSTQPPIPPKKSK
IRESfinder result
IRESfinder score
0.479512844
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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