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mmu_circPpm1g_228
Basic information of circular RNA
PanCircBase ID:
mmu_circPpm1g_228
mm39_Chr. Position ID:
chr5|31363381|31365039|-
Gene name:
Ppm1g
Transcript:
ENSMUST00000202294.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,4
Exon/intron offsets:
0,1525
Genomic length:
1658
Exon/intron sizes in nt:
95,133
Flanking exon/introns:
chr5:31362473-31363381|chr5:31365039-31365356
Splice length:
228
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTTTACAAGATGCCTTCTTGGCTATTGATGCCAAGCTGACCACAGAGGAAGTCATTAAGGAACTGGCCCAGATTGCAGGGAGACCCACTGAAGATGAGGATGATAAAGACAAAGTAGCAGATGAGGATGATGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTTTGAGGACAGTGAAGATGAATCAGATGAGGTGGAGGAAGAGGAGGATGACAGTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTGCCTCGAGTTGCTAAG
60.11
GTGGGTCTCCCTGCAATCTG
60.39
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTGCCTCGAGTTGCTAAG
60.11
CAGTGGGTCTCCCTGCAATC
60.39
177
PCR template sequence:
TGATGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTTTGAGGACAGTGAAGATGAATCAGATGAGGTGGAGGAAGAGGAGGATGACAGTGAGGCTTTACAAGATGCCTTCTTGGCTATTGATGCCAAGCTGACCACAGAGGAAGTCATTAAGGAACTGGCCCAGATTGCAGGGAGACCCACTGAAGATGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-223-3p(1), mmu-miR-27a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGATGACAGTGAGGCTTTACAAGATG
siRNA sets
ATCTTGTAAAGCCTCACTG, CTTGTAAAGCCTCACTGTC, TAAAGCCTCACTGTCATCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPM1G_228_ORF_1
1.2826
-0.141037661
0.084370015
147
ORF sequence:
ATGAGGATGATGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTTTGAGGACAGTGAAGATGAATCAGATGAGGTGGAGGAAGAGGAGGATGACAGTGAGGCTTTACAAGATGCCTTCTTGGCTATTGATGCCAAGCTGA
Peptide sequence:
MRMMPLTSCLELLSPSSLRTVKMNQMRWRKRRMTVRLYKMPSWLLMPS
IRESfinder result
IRESfinder score
0.149796698
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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