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mmu_circPpip5k2_2415
Basic information of circular RNA
PanCircBase ID:
mmu_circPpip5k2_2415
mm39_Chr. Position ID:
chr1|97656642|97687089|-
Gene name:
Ppip5k2
Transcript:
ENSMUST00000042509.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
19
Exon/intron indexes:
21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,993,4968,6049,11544,11906,12152,15139,16245,16477,17500,18629,20624,21649,23786,26137,26968,27926,30251
Genomic length:
30447
Exon/intron sizes in nt:
1,15,12,82,24,14,21,83,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:97655227-97656642|chr1:97687089-97689149
Splice length:
2415
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CCACCAGAAAGGCAGATTGTGGTTGGAATATGTTCCATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTTAAGTACATCACAGTAGTGGTTTTTGAAGAGGAAATTATTTTGAATGAACCCGTGGAAAACTGGCCTTTATGTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGCATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAATTAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATGCTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCCCTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAGGAATATGGTATAACTAAAGCTGAAAAACTGGAGATTGCCAAAGGCTACTGTACTCCACTAGTTAGAAAAATCCGATCAGATCTTCAGAGGACACAAGATGATGATACTGTCAATAAACTCCATCCTGTGTATTCTAGAGGTGTTCTGTCTCCTGAGCGGCACGTCCGCACCAGATTATATTTTACCAGTGAGAGTCATGTGCATTCTTTACTATCCATTCTTCGGTATGGCGCCTTATGCGAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCACGTCCGCACCAGATTAT
60.81
ACAAGGAGATCCGTTCCAGA
58.06
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAGTGAGAGTCATGTGCA
58.95
ACAAGGAGATCCGTTCCAGA
58.06
154
PCR template sequence:
TCTGTCTCCTGAGCGGCACGTCCGCACCAGATTATATTTTACCAGTGAGAGTCATGTGCATTCTTTACTATCCATTCTTCGGTATGGCGCCTTATGCGATCCACCAGAAAGGCAGATTGTGGTTGGAATATGTTCCATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTTAAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
26
Interacting miRNAs (#binding sites)
mmu-miR-153-3p(2), mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-miR-103-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-25-3p(1), mmu-miR-339-5p(1), mmu-let-7d-5p(1), mmu-miR-136-5p(1), mmu-miR-96-5p(1), mmu-miR-382-5p(1), mmu-miR-425-5p(1), mmu-miR-107-3p(1), mmu-miR-98-5p(1), mmu-miR-377-3p(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-669d-5p(1), mmu-miR-466b-3p(1), mmu-miR-670-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CGCCTTATGCGATCCACCAGAAAGGC
siRNA sets
TTCTGGTGGATCGCATAAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPIP5K2_2415_ORF_1
1.0082
0.122775716
1
4794
ORF sequence:
ATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTTAAGTACATCACAGTAGTGGTTTTTGAAGAGGAAATTATTTTGAATGAACCCGTGGAAAACTGGCCTTTATGTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGCATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAATTAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATGCTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCCCTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAGGAATATGGTATAACTAAAGCTGAAAAACTGGAGATTGCCAAAGGCTACTGTACTCCACTAGTTAGAAAAATCCGATCAGATCTTCAGAGGACACAAGATGATGATACTGTCAATAAACTCCATCCTGTGTATTCTAGAGGTGTTCTGTCTCCTGAGCGGCACGTCCGCACCAGATTATATTTTACCAGTGAGAGTCATGTGCATTCTTTACTATCCATTCTTCGGTATGGCGCCTTATGCGATCCACCAGAAAGGCAGATTGTGGTTGGAATATGTTCCATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTTAAGTACATCACAGTAGTGGTTTTTGAAGAGGAAATTATTTTGAATGAACCCGTGGAAAACTGGCCTTTATGTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGCATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAATTAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATGCTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCCCTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAGGAATATGGTATAACTAAAGCTGAAAAACTGGAGATTGCCAAAGGCTACTGTACTCCACTAGTTAGAAAAATCCGATCAGATCTTCAGAGGACACAAGATGATGATACTGTCAATAAACTCCATCCTGTGTATTCTAGAGGTGTTCTGTCTCCTGAGCGGCACGTCCGCACCAGATTATATTTTACCAGTGAGAGTCATGTGCATTCTTTACTATCCATTCTTCGGTATGGCGCCTTATGCGAT
Peptide sequence:
MAKKSKSKPMKEILERISLFKYITVVVFEEEIILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSILQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKDRDFTAEDYEKLTPSGSISVIKSMHLIKNPVKTCDKVYSLIQSLTSQIRYRMEDPKSADIQLYHSETLELMLRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQEYGITKAEKLEIAKGYCTPLVRKIRSDLQRTQDDDTVNKLHPVYSRGVLSPERHVRTRLYFTSESHVHSLLSILRYGALCDPPERQIVVGICSMAKKSKSKPMKEILERISLFKYITVVVFEEEIILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSILQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKDRDFTAEDYEKLTPSGSISVIKSMHLIKNPVKTCDKVYSLIQSLTSQIRYRMEDPKSADIQLYHSETLELMLRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQEYGITKAEKLEIAKGYCTPLVRKIRSDLQRTQDDDTVNKLHPVYSRGVLSPERHVRTRLYFTSESHVHSLLSILRYGALCD
IRESfinder result
IRESfinder score
0.559282306
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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