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mmu_circPpip5k2_2203
Basic information of circular RNA
PanCircBase ID:
mmu_circPpip5k2_2203
mm39_Chr. Position ID:
chr1|97668186|97689546|-
Gene name:
Ppip5k2
Transcript:
ENSMUST00000042509.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
16
Exon/intron indexes:
17,16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,362,608,3595,4701,4933,5956,7085,9080,10105,12242,14593,15424,16382,18707,20963
Genomic length:
21360
Exon/intron sizes in nt:
18,31,22,12,68,61,10,70,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:97662833-97668186|chr1:97689546-97697579
Splice length:
2203
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAAAGGAGGATATCTGTTCCAACCCGGGCAAGCAGCAGCCTCATTGTCCTTGCGTTTTAAACTACAACAGCAATTGGGAGTTCAACAATGAAGCAATGATATCTGAGAACAAAGGAACACTGGCCAACTAAATGTCAGCACATTTCATGCGATTGGGTGAAAACACTGCAGCAGACCTGGGTGTCAGCTGGCAGCCTGAGAATGTGCTCTTCTGACACCATTTATCTGAAGCAGCTTTAGTATAGACTATTTAAACTTTATGTATGTCAAATTCAAGAAAAATGAGTGAACCCCCCAGATTCTTTGTTGGGCCCGAAGATGCGGAAATCAATCCTGGAAACTATCGTCGCTTCTTCCACCATGCTGAAGAGGAGGAGGAGGAGGAGGATGAGTCTCCACCAGAAAGGCAGATTGTGGTTGGAATATGTTCCATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTTAAGTACATCACAGTAGTGGTTTTTGAAGAGGAAATTATTTTGAATGAACCCGTGGAAAACTGGCCTTTATGTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTCAGCAGCGTGTGAAAG
59.77
TAAAACGCAAGGACAATGAGGC
59.77
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCTTTGAGTAGCTGTCAGCA
59.37
GACAATGAGGCTGCTGCTTG
59.83
142
PCR template sequence:
TGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGGTAAAAGGAGGATATCTGTTCCAACCCGGGCAAGCAGCAGCCTCATTGTCCTTGCGTTTTAAACTACAACAGCAATTGGGAGTTCAACAATGAAGCAATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
25
Interacting miRNAs (#binding sites)
mmu-miR-153-3p(2), mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-miR-199a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-25-3p(1), mmu-miR-339-5p(1), mmu-let-7d-5p(1), mmu-miR-136-5p(1), mmu-miR-96-5p(1), mmu-miR-382-5p(1), mmu-miR-425-5p(1), mmu-miR-98-5p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-466b-3p(1), mmu-miR-670-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACTACGAGAAGGTAAAAGGAGGAT
siRNA sets
ATCCTCCTTTTACCTTCTC, TTTTACCTTCTCGTAGTCC, CTTTTACCTTCTCGTAGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPIP5K2_2203_ORF_1
1.0372
0.141929561
0.99999264
2031
ORF sequence:
ATGTCAAATTCAAGAAAAATGAGTGAACCCCCCAGATTCTTTGTTGGGCCCGAAGATGCGGAAATCAATCCTGGAAACTATCGTCGCTTCTTCCACCATGCTGAAGAGGAGGAGGAGGAGGAGGATGAGTCTCCACCAGAAAGGCAGATTGTGGTTGGAATATGTTCCATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTTAAGTACATCACAGTAGTGGTTTTTGAAGAGGAAATTATTTTGAATGAACCCGTGGAAAACTGGCCTTTATGTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGGTAAAAGGAGGATATCTGTTCCAACCCGGGCAAGCAGCAGCCTCATTGTCCTTGCGTTTTAAACTACAACAGCAATTGGGAGTTCAACAATGA
Peptide sequence:
MSNSRKMSEPPRFFVGPEDAEINPGNYRRFFHHAEEEEEEEDESPPERQIVVGICSMAKKSKSKPMKEILERISLFKYITVVVFEEEIILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSILQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKDRDFTAEDYEKVKGGYLFQPGQAAASLSLRFKLQQQLGVQQ
IRESfinder result
IRESfinder score
0.527486666
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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