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mmu_circPpip5k2_1542
Basic information of circular RNA
PanCircBase ID:
mmu_circPpip5k2_1542
mm39_Chr. Position ID:
chr1|97661610|97678453|-
Gene name:
Ppip5k2
Transcript:
ENSMUST00000042509.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
19,18,17,16,15,14,13,12,11,10,9,8
Exon/intron offsets:
0,1081,6576,6938,7184,10171,11277,11509,12532,13661,15656,16681
Genomic length:
16843
Exon/intron sizes in nt:
22,41,42,18,31,22,12,60,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:97657763-97661610|chr1:97678453-97680428
Splice length:
1542
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGCATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAATTAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATGCTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCCCTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGTTCAGCATAACGGTTCCT
58.28
CTGTCTCGCTCTACCTTGCC
60.18
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTTCAGCATAACGGTTCCT
58.28
CTTCGCTGTCTCGCTCTACC
60.25
173
PCR template sequence:
CATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-153-3p(2), mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-miR-103-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-25-3p(1), mmu-miR-339-5p(1), mmu-let-7d-5p(1), mmu-miR-96-5p(1), mmu-miR-382-5p(1), mmu-miR-425-5p(1), mmu-miR-107-3p(1), mmu-miR-98-5p(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-466b-3p(1), mmu-miR-670-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTTATCCCTCAGGTTTATACCGTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPIP5K2_1542_ORF_1
1.0317
0.122054496
0.999999976
2835
ORF sequence:
ATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGCATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAATTAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATGCTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCCCTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTCAAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTCTAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAGTGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGACAGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGCATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAATTAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATGCTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCCCTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGAACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAG
Peptide sequence:
MKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKDRDFTAEDYEKLTPSGSISVIKSMHLIKNPVKTCDKVYSLIQSLTSQIRYRMEDPKSADIQLYHSETLELMLRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKDRDFTAEDYEKLTPSGSISVIKSMHLIKNPVKTCDKVYSLIQSLTSQIRYRMEDPKSADIQLYHSETLELMLRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQ
IRESfinder result
IRESfinder score
0.528977054
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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