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mmu_circPpip5k2_1305
Basic information of circular RNA
PanCircBase ID:
mmu_circPpip5k2_1305
mm39_Chr. Position ID:
chr1|97668794|97684659|-
Gene name:
Ppip5k2
Transcript:
ENSMUST00000042509.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,2987,4093,4325,5348,6477,8472,9497,11634,13985,14816,15774
Genomic length:
15865
Exon/intron sizes in nt:
126,86,110,76,87,102,122,162,102,155,86,91
Flanking exon/introns:
chr1:97668670-97668794|chr1:97684659-97686893
Splice length:
1305
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAATGGGGAGGAGAACTGACC
59.36
ACAAATGGATTCCTGAGTTTTGCA
59.66
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAATGGGGAGGAGAACTGACC
59.96
ACAAATGGATTCCTGAGTTTTGCA
59.66
141
PCR template sequence:
CTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-153-3p(2), mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-196a-5p(1), mmu-let-7d-5p(1), mmu-miR-136-5p(1), mmu-miR-96-5p(1), mmu-miR-382-5p(1), mmu-miR-425-5p(1), mmu-miR-98-5p(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-466b-3p(1), mmu-miR-670-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGGAGGTCAAGGATTTCCACTGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPIP5K2_1305_ORF_1
1.0814
0.154768354
0.999999844
2541
ORF sequence:
ATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATTCTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAATGCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAAGCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGACTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCATATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGCACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTAAGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAACGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTTGCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCTCTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAAAATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGGAAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGCTGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGAGATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAAACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAGAACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGGTCAA
Peptide sequence:
MQYLIQDRRDVYSILQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSILQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQ
IRESfinder result
IRESfinder score
0.466495425
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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