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mmu_circPpig_787
Basic information of circular RNA
PanCircBase ID:
mmu_circPpig_787
mm39_Chr. Position ID:
chr2|69563161|69574124|+
Gene name:
Ppig
Transcript:
ENSMUST00000090858.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
5,6,7,8,9,10,11
Exon/intron offsets:
0,371,1473,3050,3171,8668,10795
Genomic length:
10963
Exon/intron sizes in nt:
1,08,45,88,30,14,02,08,000
Flanking exon/introns:
chr2:69562798-69563161|chr2:69574124-69576371
Splice length:
787
Other database IDs:
circAtlas: mmu-Ppig_0006
Human Ortholog IDs:
Mature spliced Sequence:
GTGAAAAAGGGACAGGGAAATCAACTCAGAAGCCATTACATTATAAGAGCTGTCTCTTTCATAGAGTTGTCAAGGATTTCATGGTTCAGGGTGGTGACTTCAGTGAAGGGAATGGAAGAGGAGGAGAATCTATTTATGGAGGATTCTTTGAAGATGAGAGTTTTGCTGTTAAACACAACAAAGAGTTTCTCTTATCAATGGCCAACAGAGGGAAGGATACAAATGGCTCTCAGTTCTTTATAACAACAAAACCAACTCCTCATTTAGATGGGCATCATGTTGTTTTTGGGCAAGTGATCTCTGGTCAGGAAGTTGTGAGAGAGATTGAAAACCAGAAAACAGATGCCGCTAGCAAACCATTTGCTGAAGTTCGGATACTCAGTTGCGGAGAGCTGATTCCAAAGTCTAAGGTTAAGAAAGAAGAAAAGAAAAGGCATAAGTCCTCGTCCTCCTCCTCCTCCAGTGACTCAGACAGCTCAAGCGATTCTCAGTCCTCCTCTGAGTCTTCTGATTCTGAAAGCGCTTCCGAAGAAAAATCCAGAAAAAGGAAAAAGAAACATAGGAAAAATTCCCGAAAGCATAAGAAGGAGAAGAAGAAGCGAAAGAAAAGCAAGAAAAGCCCATCTAGTGAAAGTGAAGCAGAAAATGTTGATGCACAGCCCCAGTCTACTGTCCGTCCAGAAGAGATCCCTCCTATACCAGAAAATAGATTTCTAATGAGAAAAAGTCCTCCTAAAGCCGATGACAAGGAGAGGAAAAACAGAGAGAGAGAAAGAGAAAGAGAGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCGATGACAAGGAGAGGA
60.32
GTCACCACCCTGAACCATGA
59.6
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCGATGACAAGGAGAGGA
60.32
AGTCACCACCCTGAACCATG
59.6
150
PCR template sequence:
TCCCTCCTATACCAGAAAATAGATTTCTAATGAGAAAAAGTCCTCCTAAAGCCGATGACAAGGAGAGGAAAAACAGAGAGAGAGAAAGAGAAAGAGAGTGGTGAAAAAGGGACAGGGAAATCAACTCAGAAGCCATTACATTATAAGAGCTGTCTCTTTCATAGAGTTGTCAAGGATTTCATGGTTCAGGGTGGTGACTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-298-5p(1), mmu-miR-362-5p(1), mmu-miR-340-5p(1), mmu-miR-680(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GAGAAAGAGAGTGGTGAAAAAGGGAC
siRNA sets
TTTTCACCACTCTCTTTCT, TTTTTCACCACTCTCTTTC, TTTCACCACTCTCTTTCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPIG_787_ORF_1
0.9136
0.11221086
0.946577238
831
ORF sequence:
ATGACAAGGAGAGGAAAAACAGAGAGAGAGAAAGAGAAAGAGAGTGGTGAAAAAGGGACAGGGAAATCAACTCAGAAGCCATTACATTATAAGAGCTGTCTCTTTCATAGAGTTGTCAAGGATTTCATGGTTCAGGGTGGTGACTTCAGTGAAGGGAATGGAAGAGGAGGAGAATCTATTTATGGAGGATTCTTTGAAGATGAGAGTTTTGCTGTTAAACACAACAAAGAGTTTCTCTTATCAATGGCCAACAGAGGGAAGGATACAAATGGCTCTCAGTTCTTTATAACAACAAAACCAACTCCTCATTTAGATGGGCATCATGTTGTTTTTGGGCAAGTGATCTCTGGTCAGGAAGTTGTGAGAGAGATTGAAAACCAGAAAACAGATGCCGCTAGCAAACCATTTGCTGAAGTTCGGATACTCAGTTGCGGAGAGCTGATTCCAAAGTCTAAGGTTAAGAAAGAAGAAAAGAAAAGGCATAAGTCCTCGTCCTCCTCCTCCTCCAGTGACTCAGACAGCTCAAGCGATTCTCAGTCCTCCTCTGAGTCTTCTGATTCTGAAAGCGCTTCCGAAGAAAAATCCAGAAAAAGGAAAAAGAAACATAGGAAAAATTCCCGAAAGCATAAGAAGGAGAAGAAGAAGCGAAAGAAAAGCAAGAAAAGCCCATCTAGTGAAAGTGAAGCAGAAAATGTTGATGCACAGCCCCAGTCTACTGTCCGTCCAGAAGAGATCCCTCCTATACCAGAAAATAGATTTCTAATGAGAAAAAGTCCTCCTAAAGCCGATGACAAGGAGAGGAAAAACAGAGAGAGAGAAAGAGAAAGAGAG
Peptide sequence:
MTRRGKTEREKEKESGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSDSDSSSDSQSSSESSDSESASEEKSRKRKKKHRKNSRKHKKEKKKRKKSKKSPSSESEAENVDAQPQSTVRPEEIPPIPENRFLMRKSPPKADDKERKNRERERERE
IRESfinder result
IRESfinder score
0.509851536
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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