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mmu_circPolq_1272
Basic information of circular RNA
PanCircBase ID:
mmu_circPolq_1272
mm39_Chr. Position ID:
chr16|36833468|36866236|+
Gene name:
Polq
Transcript:
ENSMUST00000071452.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
2,3,4,5,6,7,8,9
Exon/intron offsets:
0,1972,4092,27376,28615,29007,31631,32643
Genomic length:
32768
Exon/intron sizes in nt:
1,80,13,11,57,13,72,05,00,00,00,000
Flanking exon/introns:
chr16:36832421-36833468|chr16:36866236-36868900
Splice length:
1272
Other database IDs:
circAtlas: mmu-Polq_0019
Human Ortholog IDs:
Mature spliced Sequence:
GAACAAACAAGCGAAGAGTTTCTGATGACCAGATAGACCAGCTGCTGTTGGCAAATTGGGGACTTCCTAAAGCAGTTCTGGAGAAATACCATAGTTTTGGTGTAAGAAAGATGTTCGAATGGCAGGCAGAATGCCTTTTGCTTGGTCACGTCTTGGAAGGAAAGAATCTAGTTTATTCAGCTCCTACAAGTGCTGGGAAGACTCTTGTTGCAGAGTTACTTATTCTGAAGCGGGTTTTGGAAACACGGAAGAAAGCGTTGTTTATTTTGCCCTTTGTGTCTGTGGCTAAAGAGAAGAAATGCTACCTCCAGAGTCTGTTTCAGGAAGTAGGACTAAAGGTAGATGGCTATATGGGCAGCACCTCACCAACGGGACAGTTCTCCTCCTTGGACATTGCTGTTTGCACTATTGAGAGAGCCAATGGGCTGGTCAATCGCCTCATTGAGGAAAATAAGATGGATCTGTTAGGTGAAAGTATCCTAGTTTGTAAGAACTCTGAGAAATCCAAGGGCATTGCTCTGCTTCAAGGATCTCTGGAGCCTGTGCACAGCTGTCTGCAGAGACAGGGAGAAGTCACTGCTAGCATGATACGAGCTATTCTGGAGATAATCGTTGGTGGGGTGGCCAGTACGTCACAAGATATGCAGACTTATGCCGCCTGCACCTTCTTGGCTGCTGCTATACAGGAAGGGAAGCAAGGGATGCAGAGAAATCAAGATGATGCTCAGCTTGGAGCGATTGATGCCTGTGTGACGTGGCTGTTAGAAAATGAGTTCATCCAGGTGGCAGAGCCCGGGGACGGCACAGGAGGAAAGGTGTATCACCCAACACATCTTGGATCAGCTACTCTTTCTTCTTCACTGTCTCCAACTGATACCCTTGATATTTTTGCTGACCTCCAAAGAGCAATGAAAGGCTTTGTTTTAGAGAATGACCTTCATATTGTCTACCTGGTTACACCTGTGTTTGAAGATTGGATTAGTATTGATTGGTACCGATTTTTCTGTTTATGGGAAAAATTACCAACTTCTATGAAAAGGGTGGCAGAGCTGGTTGGAGTGGAGGAAGGGTTCTTGGCTCGATGTGTGAAAGGAAAAGTAGTAGCTAGAACTGAGAGACAGCATCGGCAGATGGCCATCCATAAAAGGTTTTTCACAAGTCTTGTGTTACTGGATTTAATCAGTGAAATTCCCTTAAAGGACATTAATCAGAAGTATGGATGTAATCGTGGACAGATTCAGTCTTTGCAACAGTCAGCTGCTGTCTATGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGTGAAATTCCCTTAAAGGACA
58.05
GTCCCCAATTTGCCAACAGC
60.32
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGTGAAATTCCCTTAAAGGACA
58.05
GCCAACAGCAGCTGGTCTAT
60.39
145
PCR template sequence:
GATTTAATCAGTGAAATTCCCTTAAAGGACATTAATCAGAAGTATGGATGTAATCGTGGACAGATTCAGTCTTTGCAACAGTCAGCTGCTGTCTATGCAGGAACAAACAAGCGAAGAGTTTCTGATGACCAGATAGACCAGCTGCTGTTGGCAAATTGGGGACTTCCTAAAGCAGTTCTGGAGAAATACCATAGTTTTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-15b-5p(1), mmu-miR-146a-5p(1), mmu-miR-217-5p(1), mmu-miR-339-5p(1), mmu-miR-153-3p(1), mmu-miR-301b-3p(1), mmu-miR-758-3p(1), mmu-miR-362-5p(1), mmu-miR-301a-3p(1), mmu-miR-98-5p(1), mmu-miR-802-5p(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTGTCTATGCAGGAACAAACAAGCG
siRNA sets
TTGTTTGTTCCTGCATAGA, CTTGTTTGTTCCTGCATAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPOLQ_1272_ORF_1
1.0186
0.105023586
0.999999534
2433
ORF sequence:
ATGTTCGAATGGCAGGCAGAATGCCTTTTGCTTGGTCACGTCTTGGAAGGAAAGAATCTAGTTTATTCAGCTCCTACAAGTGCTGGGAAGACTCTTGTTGCAGAGTTACTTATTCTGAAGCGGGTTTTGGAAACACGGAAGAAAGCGTTGTTTATTTTGCCCTTTGTGTCTGTGGCTAAAGAGAAGAAATGCTACCTCCAGAGTCTGTTTCAGGAAGTAGGACTAAAGGTAGATGGCTATATGGGCAGCACCTCACCAACGGGACAGTTCTCCTCCTTGGACATTGCTGTTTGCACTATTGAGAGAGCCAATGGGCTGGTCAATCGCCTCATTGAGGAAAATAAGATGGATCTGTTAGGTGAAAGTATCCTAGTTTGTAAGAACTCTGAGAAATCCAAGGGCATTGCTCTGCTTCAAGGATCTCTGGAGCCTGTGCACAGCTGTCTGCAGAGACAGGGAGAAGTCACTGCTAGCATGATACGAGCTATTCTGGAGATAATCGTTGGTGGGGTGGCCAGTACGTCACAAGATATGCAGACTTATGCCGCCTGCACCTTCTTGGCTGCTGCTATACAGGAAGGGAAGCAAGGGATGCAGAGAAATCAAGATGATGCTCAGCTTGGAGCGATTGATGCCTGTGTGACGTGGCTGTTAGAAAATGAGTTCATCCAGGTGGCAGAGCCCGGGGACGGCACAGGAGGAAAGGTGTATCACCCAACACATCTTGGATCAGCTACTCTTTCTTCTTCACTGTCTCCAACTGATACCCTTGATATTTTTGCTGACCTCCAAAGAGCAATGAAAGGCTTTGTTTTAGAGAATGACCTTCATATTGTCTACCTGGTTACACCTGTGTTTGAAGATTGGATTAGTATTGATTGGTACCGATTTTTCTGTTTATGGGAAAAATTACCAACTTCTATGAAAAGGGTGGCAGAGCTGGTTGGAGTGGAGGAAGGGTTCTTGGCTCGATGTGTGAAAGGAAAAGTAGTAGCTAGAACTGAGAGACAGCATCGGCAGATGGCCATCCATAAAAGGTTTTTCACAAGTCTTGTGTTACTGGATTTAATCAGTGAAATTCCCTTAAAGGACATTAATCAGAAGTATGGATGTAATCGTGGACAGATTCAGTCTTTGCAACAGTCAGCTGCTGTCTATGCAGGAACAAACAAGCGAAGAGTTTCTGATGACCAGATAGACCAGCTGCTGTTGGCAAATTGGGGACTTCCTAAAGCAGTTCTGGAGAAATACCATAGTTTTGGTGTAAGAAAGATGTTCGAATGGCAGGCAGAATGCCTTTTGCTTGGTCACGTCTTGGAAGGAAAGAATCTAGTTTATTCAGCTCCTACAAGTGCTGGGAAGACTCTTGTTGCAGAGTTACTTATTCTGAAGCGGGTTTTGGAAACACGGAAGAAAGCGTTGTTTATTTTGCCCTTTGTGTCTGTGGCTAAAGAGAAGAAATGCTACCTCCAGAGTCTGTTTCAGGAAGTAGGACTAAAGGTAGATGGCTATATGGGCAGCACCTCACCAACGGGACAGTTCTCCTCCTTGGACATTGCTGTTTGCACTATTGAGAGAGCCAATGGGCTGGTCAATCGCCTCATTGAGGAAAATAAGATGGATCTGTTAGGTGAAAGTATCCTAGTTTGTAAGAACTCTGAGAAATCCAAGGGCATTGCTCTGCTTCAAGGATCTCTGGAGCCTGTGCACAGCTGTCTGCAGAGACAGGGAGAAGTCACTGCTAGCATGATACGAGCTATTCTGGAGATAATCGTTGGTGGGGTGGCCAGTACGTCACAAGATATGCAGACTTATGCCGCCTGCACCTTCTTGGCTGCTGCTATACAGGAAGGGAAGCAAGGGATGCAGAGAAATCAAGATGATGCTCAGCTTGGAGCGATTGATGCCTGTGTGACGTGGCTGTTAGAAAATGAGTTCATCCAGGTGGCAGAGCCCGGGGACGGCACAGGAGGAAAGGTGTATCACCCAACACATCTTGGATCAGCTACTCTTTCTTCTTCACTGTCTCCAACTGATACCCTTGATATTTTTGCTGACCTCCAAAGAGCAATGAAAGGCTTTGTTTTAGAGAATGACCTTCATATTGTCTACCTGGTTACACCTGTGTTTGAAGATTGGATTAGTATTGATTGGTACCGATTTTTCTGTTTATGGGAAAAATTACCAACTTCTATGAAAAGGGTGGCAGAGCTGGTTGGAGTGGAGGAAGGGTTCTTGGCTCGATGTGTGAAAGGAAAAGTAGTAGCTAGAACTGAGAGACAGCATCGGCAGATGGCCATCCATAAAAGGTTTTTCACAAGTCTTGTGTTACTGGATTTAATCAGTGAAATTCCCTTAAAGGACATTAATCAGAAGTATGGATGTAATCGTGGACAGATTCAGTCTTTGCAACAGTCAGCTGCTGTCTATGCA
Peptide sequence:
MFEWQAECLLLGHVLEGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKCYLQSLFQEVGLKVDGYMGSTSPTGQFSSLDIAVCTIERANGLVNRLIEENKMDLLGESILVCKNSEKSKGIALLQGSLEPVHSCLQRQGEVTASMIRAILEIIVGGVASTSQDMQTYAACTFLAAAIQEGKQGMQRNQDDAQLGAIDACVTWLLENEFIQVAEPGDGTGGKVYHPTHLGSATLSSSLSPTDTLDIFADLQRAMKGFVLENDLHIVYLVTPVFEDWISIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEIPLKDINQKYGCNRGQIQSLQQSAAVYAGTNKRRVSDDQIDQLLLANWGLPKAVLEKYHSFGVRKMFEWQAECLLLGHVLEGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKCYLQSLFQEVGLKVDGYMGSTSPTGQFSSLDIAVCTIERANGLVNRLIEENKMDLLGESILVCKNSEKSKGIALLQGSLEPVHSCLQRQGEVTASMIRAILEIIVGGVASTSQDMQTYAACTFLAAAIQEGKQGMQRNQDDAQLGAIDACVTWLLENEFIQVAEPGDGTGGKVYHPTHLGSATLSSSLSPTDTLDIFADLQRAMKGFVLENDLHIVYLVTPVFEDWISIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEIPLKDINQKYGCNRGQIQSLQQSAAVYA
IRESfinder result
IRESfinder score
0.59506092
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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