top of page
mmu_circPola2_903
Basic information of circular RNA
PanCircBase ID:
mmu_circPola2_903
mm39_Chr. Position ID:
chr19|5992023|6001814|-
Gene name:
Pola2
Transcript:
ENSMUST00000025752.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
17,16,15,14,13,12,11,10,9,8
Exon/intron offsets:
0,354,1227,1559,4474,5708,6406,8450,9146,9635
Genomic length:
9791
Exon/intron sizes in nt:
12,76,01,07,10,97,43,90,00,00,00,000
Flanking exon/introns:
chr19:5991045-5992023|chr19:6001814-6003092
Splice length:
903
Other database IDs:
circAtlas: mmu-Pola2_0003
circBase: hsa_circ_0096120, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_128344
Human Ortholog IDs:
Mature spliced Sequence:
GAGCCTGTTATTCTCCTGGGTCAGATTGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTATGGCGCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTATGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTTCTATCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACCACCTCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATGTCTGCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCCATTCGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTTGTCTTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCGAGCTGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGTGATGTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGGACGTCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCACCCCACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTTCATAGTGCCGTCCGAGCTGAGATACTTTGTGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATACCTATGCCCAGCTGCC
59.02
CTGGTCCCCCTCGAGAATCA
60.69
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTATACCTATGCCCAGCTGCC
60.07
CTGGTCCCCCTCGAGAATCA
60.69
157
PCR template sequence:
GGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTTCATAGTGCCGTCCGAGCTGAGATACTTTGTGAAGGAGCCTGTTATTCTCCTGGGTCAGATTGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTATGGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1), mmu-miR-433-3p(1), mmu-miR-483-5p(1), mmu-miR-192-5p(1), mmu-miR-705(1)
siRNAs for circular RNA silencing
Junction sequence
ATACTTTGTGAAGGAGCCTGTTATTC
siRNA sets
AATAACAGGCTCCTTCACA, GAATAACAGGCTCCTTCAC, ATAACAGGCTCCTTCACAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPOLA2_903_ORF_1
1.1827
0.34963313
0.999962291
1641
ORF sequence:
ATGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTTCTATCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACCACCTCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATGTCTGCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCCATTCGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTTGTCTTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCGAGCTGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGTGATGTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGGACGTCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCACCCCACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTTCATAGTGCCGTCCGAGCTGAGATACTTTGTGAAGGAGCCTGTTATTCTCCTGGGTCAGATTGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTATGGCGCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTATGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTTCTATCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACCACCTCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATGTCTGCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCCATTCGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTTGTCTTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCGAGCTGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGTGATGTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGGACGTCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCACCCCACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTTCATAGTGCCGTCCGAGCTGAGATACTTTGTGAAG
Peptide sequence:
MEGFNTTGRRLTATKLYEGVPLPFYQPTEEEGASEQTMVVVACGPYTTSDSITYDPLLDLIAIINRDQPDVCILFGPFLDAKHEQVENCKLTSPFEDVFKQCLRTVIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFTFSELSREDKKRVQFVSEPCSLSINGVMFGLTSTDLLFHIGAEEIFSSSGTSDRFSRVLKHILTQRSYYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKEPVILLGQIGCDSNGKLNSKSVILEGDQEHSYGAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLYEGVPLPFYQPTEEEGASEQTMVVVACGPYTTSDSITYDPLLDLIAIINRDQPDVCILFGPFLDAKHEQVENCKLTSPFEDVFKQCLRTVIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFTFSELSREDKKRVQFVSEPCSLSINGVMFGLTSTDLLFHIGAEEIFSSSGTSDRFSRVLKHILTQRSYYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVK
IRESfinder result
IRESfinder score
0.481406748
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page