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mmu_circPoglut1_341
Basic information of circular RNA
PanCircBase ID:
mmu_circPoglut1_341
mm39_Chr. Position ID:
chr16|38352145|38363321|-
Gene name:
Poglut1
Transcript:
ENSMUST00000036210.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,2941,6123,11054
Genomic length:
11176
Exon/intron sizes in nt:
5,91,00,60,122
Flanking exon/introns:
chr16:38349974-38352145|chr16:38363321-38366110
Splice length:
341
Other database IDs:
circBase: mmu_circ_0006269, CIRCpedia: MMU_CIRCpedia_216468, circAtlas: mmu-Poglut1_0002
Human Ortholog IDs:
Mature spliced Sequence:
ACATCGGAGTACCATGATATCATGTATCCTGCGTGGACATTTTGGGAAGGGGGCCCTGCTGTGTGGCCACTTTATCCTACAGGTCTTGGACGGTGGGACCTCTTCAGAGAAGACCTGTTAAGGTCAGCAGCGCAATGGCCGTGGGAAAAGAAAAATTCTACAGCATATTTCCGAGGATCAAGGACAAGCCCAGAAAGAGATCCTCTCATTCTCCTATCTCGGAAAAATCCAAAGCTCGTCGATGCCGAGTACACCAAAAACCAGGCCTGGAAGTCTATGAAAGATACTCTGGGAAAGCCAGCCGCTAAGGATGTACACCTCATAGATCACTGCAAATACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATACTCTGGGAAAGCCAGCC
59.45
ACCGTCCAAGACCTGTAGGA
59.89
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATACTCTGGGAAAGCCAGCC
59.45
CACCGTCCAAGACCTGTAGG
59.75
153
PCR template sequence:
ATGCCGAGTACACCAAAAACCAGGCCTGGAAGTCTATGAAAGATACTCTGGGAAAGCCAGCCGCTAAGGATGTACACCTCATAGATCACTGCAAATACAGACATCGGAGTACCATGATATCATGTATCCTGCGTGGACATTTTGGGAAGGGGGCCCTGCTGTGTGGCCACTTTATCCTACAGGTCTTGGACGGTGGGACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-296-5p(1), mmu-let-7e-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTGCAAATACAGACATCGGAGTACC
siRNA sets
CTCCGATGTCTGTATTTGC, TCCGATGTCTGTATTTGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPOGLUT1_341_ORF_1
0.4588
0.031997897
0.251115792
441
ORF sequence:
ATGTATCCTGCGTGGACATTTTGGGAAGGGGGCCCTGCTGTGTGGCCACTTTATCCTACAGGTCTTGGACGGTGGGACCTCTTCAGAGAAGACCTGTTAAGGTCAGCAGCGCAATGGCCGTGGGAAAAGAAAAATTCTACAGCATATTTCCGAGGATCAAGGACAAGCCCAGAAAGAGATCCTCTCATTCTCCTATCTCGGAAAAATCCAAAGCTCGTCGATGCCGAGTACACCAAAAACCAGGCCTGGAAGTCTATGAAAGATACTCTGGGAAAGCCAGCCGCTAAGGATGTACACCTCATAGATCACTGCAAATACAGACATCGGAGTACCATGATATCATGTATCCTGCGTGGACATTTTGGGAAGGGGGCCCTGCTGTGTGGCCACTTTATCCTACAGGTCTTGGACGGTGGGACCTCTTCAGAGAAGACCTGTTAA
Peptide sequence:
MYPAWTFWEGGPAVWPLYPTGLGRWDLFREDLLRSAAQWPWEKKNSTAYFRGSRTSPERDPLILLSRKNPKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLIDHCKYRHRSTMISCILRGHFGKGALLCGHFILQVLDGGTSSEKTC
IRESfinder result
IRESfinder score
0.358347975
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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