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mmu_circPlcg1_3618
Basic information of circular RNA
PanCircBase ID:
mmu_circPlcg1_3618
mm39_Chr. Position ID:
chr2|160589694|160603843|+
Gene name:
Plcg1
Transcript:
ENSMUST00000151590.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
30
Exon/intron indexes:
3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32
Exon/intron offsets:
0,259,507,3188,3368,3661,3858,4009,4313,4520,4679,5410,5667,5868,6049,6510,6788,6986,7966,8766,9095,9439,10197,11679,11907,12208,13305,13530,13785,13964
Genomic length:
14149
Exon/intron sizes in nt:
1,53,94,48,85,84,35,73,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:160573953-160589694|chr2:160603843-160604013
Splice length:
3618
Other database IDs:
CIRCpedia: MMU_CIRCpedia_11134
Human Ortholog IDs:
Mature spliced Sequence:
TTGATATCCGAGAAATCAAGGAGATCCGTCCAGGGAAGACTTCTCGGGACTTTGACCGCTACCAAGAAGACCCTGCCTTCCGGCCAGATCAATCACACTGCTTTGTCATCCTCTATGGAATGGAATTCCGCTTGAAGACCCTGAGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTAATGGAGGATACACTGCAGGCAGCCACACCCCTGCAAATTGAGAGGTGGCTCCGGAAGCAGTTCTACTCAGTGGATCGTAACCGAGAGGATCGTATATCAGCCAAGGACTTGAAGAACATGCTGTCTCAGGTCAACTACCGAGTCCCCAATATGCGCTTCCTCCGAGAGCGGCTGACGGACCTTGAACAGCGCAGCGGGGACATCACCTACGGGCAGTTTGCTCAGCTGTACCGCAGCCTCATGTACAGCGCCCAGAAGACGATGGACCTTCCCTTCTTGGAAACCAACGCTTTGAGGACTGGAGAGCGTCCGGAGCATTGCCAGGTGTCCCTTTCTGAGTTCCAGCAGTTCCTCCTTGAATACCAGGGGGAGCTGTGGGCTGTCGACCGGCTCCAGGTGCAGGAATTCATGCTCAGCTTCCTTCGAGACCCCTTGCGAGAGATTGAGGAGCCATACTTCTTCTTGGATGAGCTTGTCACCTTCCTGTTCTCCAAAGAGAACAGCGTGTGGAACTCACAGCTGGATGCTGTGTGCCCGGACACCATGAACAACCCACTCTCTCACTATTGGATCTCCTCCTCACATAATACGTACCTGACTGGGGACCAGTTCTCCAGTGAGTCCTCCCTGGAAGCCTATGCTCGCTGCCTGAGGATGGGCTGTCGCTGCATTGAGTTGGACTGCTGGGATGGGCCAGATGGGATGCCAGTCATTTACCATGGGCACACTCTCACCACCAAGATCAAGTTCTCTGACGTCCTGCACACCATCAAGGAGCATGCTTTCGTAGCCTCAGAGTACCCAGTCATCCTGTCCATCGAGGACCACTGCAGCATTGCCCAGCAGAGAAACATGGCTCAGCACTTCAGAAAGGTGCTTGGCGACACACTCCTTACCAAGCCTGTGGACATTGCTGCTGATGGCCTTCCTTCTCCCAACCAGCTCAGGAGGAAGATCCTTATTAAGCACAAGAAGCTGGCTGAGGGCAGTGCCTATGAGGAGGTGCCTACCTCTGTGATGTACTCCGAGAATGACATCAGTAACTCCATCAAGAATGGTATCCTCTACTTGGAGGACCCCGTGAATCATGTGAGGAGTGGTATCCCCACTACTTCGTTCTGACTAGCAGCAAGATCTACTACTCTGAGGAGACCAGCAGTGACCAGGGCAATGAGGATGAAGAGGAGCCGAAGGAGGCCAGCAGCAGCACAGAGCTGCACTCGAGTGAGAAGTGGTTCCACGGGAAGCTCGGGGCTGGACGTGACGGGCGGCACATTGCTGAGCGCCTGCTCACTGAGTACTGCATAGAGACTGGGGCTCCTGATGGCTCCTTCCTAGTGCGAGAAAGTGAGACCTTCGTGGGTGACTACACGCTGTCTTTCTGGCGGAATGGGAAAGTCCAGCACTGCCGCATCCACTCCCGGCAGGATGCTGGGACTCCTAAGTTCTTCTTGACAGATAACCTTGTCTTTGACTCTCTCTATGACCTCATCACACATTATCAGCAAGTACCCCTGCGCTGCAATGAGTTTGAGATGCGCCTTTCAGAGCCTGTTCCACAGACGAATGCCCATGAGAGCAAAGAGTGGTACCACGCAAGCCTGACTAGAGCTCAGGCTGAACATATGCTGATGCGAGTGCCCCGGGATGGGGCCTTCCTGGTGCGGAAACGCAATGAGCCTAACTCATATGCCATCTCTTTCCGGGCTGAGGGAAAGATCAAGCACTGCCGAGTACAGCAGGAAGGCCAGACAGTGATGCTGGGGAACTCTGAGTTTGACAGCCTGGTTGACCTCATCAGCTACTATGAGAAGCACCCCCTGTACCGCAAAATGAAGCTACGCTACCCCATCAACGAGGAGGCACTGGAGAAGATCGGGACAGCTGAACCCGATTATGGGGCACTATACGAGGGCCGCAACCCTGGTTTCTATGTGGAGGCAAACCCTATGCCAACTTTCAAGTGTGCAGTAAAAGCCCTCTTCGACTACAAGGCCCAGAGAGAGGATGAGCTGACCTTCACCAAGAGTGCCATCATCCAGAATGTGGAAAAGCAAGATGGTGGCTGGTGGCGAGGGGACTATGGTGGGAAGAAGCAGCTGTGGTTCCCCTCAAACTATGTGGAAGAGATGATCAATCCAGCAGTCCTAGAGCCTGAGAGGGAGCACCTGGATGAGAACAGCCCACTGGGGGACTTGCTGCGAGGGGTCTTAGATGTGCCAGCTTGTCAGATCGCCATCCGTCCTGAGGGCAAAAACAACCGGCTCTTCGTCTTCTCCATCAGCATGCCATCAGTGGCTCAGTGGTCCCTGGATGTTGCAGCTGACTCACAGGAGGAGTTACAGGACTGGGTGAAAAAGATCCGTGAAGTTGCCCAGACTGCAGATGCCAGGCTCACTGAGGGAAAGATGATGGAGAGGAGGAAGAAGATCGCCTTGGAGCTCTCCGAGCTTGTGGTCTACTGCCGGCCCGTTCCCTTTGATGAAGAGAAGATTGGCACAGAACGTGCTTGTTACCGGGACATGTCCTCCTTTCCGGAAACCAAGGCTGAGAAGTATGTGAACAAGGCCAAAGGCAAGAAGTTCCTCCAGTACAACCGGCTGCAGCTCTCGCGCATCTACCCTAAGGGCCAGAGGCTAGACTCCTCCAATTATGACCCTCTGCCCATGTGGATCTGCGGTAGCCAGCTTGTAGCACTCAATTTCCAGACCCCAGACAAGCCTATGCAGATGAACCAGGCCCTCTTCATGGCTGGTGGGCATTGTGGCTATGTGCTGCAGCCAAGCACCATGAGAGACGAAGCCTTTGACCCCTTTGATAAGAGCAGTCTCCGAGGTCTGGAACCCTGTGTCATTTGCATTGAGGTGCTGGGGGCCAGGCATCTGCCGAAGAATGGCCGGGGTATTGTGTGTCCTTTTGTGGAGATTGAGGTGGCTGGGGCTGAGTACGACAGCACCAAGCAAAAGACGGAGTTTGTAGTGGACAACGGACTGAACCCTGTGTGGCCTGCTAAGCCCTTCCACTTCCAGATCAGTAACCCAGAGTTTGCCTTTCTGCGCTTTGTGGTGTATGAGGAAGACATGTTTAGTGACCAGAACTTCTTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATATAGAGCAGTGCCTTTGAAGAACAACTACAGTGAAGACCTGGAGTTGGCCTCCCTGCTCATCAAGATTGACATTTTCCCTGCTAAGGAGAACGGTGACCTCAGTCCTTTCAGTGGCATATCCCTAAGGGAACGGGCCTCAGATGCCTCCAGCCAGCTGTTCCATGTCCGGGCCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGATTTCCGCATCTCGCAGGAGCATCTAGCAGACCATTTTGACAGTCGGGAACGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGCCAGATACCAGCAGC
60.11
TTGGTAGCGGTCAAAGTCCC
59.96
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGGGTCCTTTGAAGCCA
59.89
TTGGTAGCGGTCAAAGTCCC
59.96
161
PCR template sequence:
CCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGATTTCCGCATCTCGCAGGAGCATCTAGCAGACCATTTTGACAGTCGGGAACGAAGTTGATATCCGAGAAATCAAGGAGATCCGTCCAGGGAAGACTTCTCGGGACTTTGACCGCTACCAAGAAGACCCTGCCTTCCGGCCAGATCAATCACACTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
31
Interacting miRNAs (#binding sites)
mmu-miR-146a-5p(2), mmu-miR-218-5p(2), mmu-let-7a-5p(1), mmu-miR-32-5p(1), mmu-let-7e-5p(1), mmu-miR-34a-5p(1), mmu-miR-122-5p(1), mmu-miR-322-5p(1), mmu-miR-298-5p(1), mmu-miR-184-3p(1), mmu-miR-324-5p(1), mmu-miR-326-3p(1), mmu-miR-138-5p(1), mmu-let-7d-5p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-351-5p(1), mmu-miR-370-3p(1), mmu-miR-183-5p(1), mmu-miR-324-3p(1), mmu-miR-342-5p(1), mmu-miR-27a-5p(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-484(1), mmu-miR-452-3p(1), mmu-miR-574-5p(1), mmu-miR-146b-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCGGGAACGAAGTTGATATCCGAGA
siRNA sets
ATATCAACTTCGTTCCCGA, GATATCAACTTCGTTCCCG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPLCG1_3618_ORF_1
1.1628
0.451153496
0.999994921
1884
ORF sequence:
ATGCGCCTTTCAGAGCCTGTTCCACAGACGAATGCCCATGAGAGCAAAGAGTGGTACCACGCAAGCCTGACTAGAGCTCAGGCTGAACATATGCTGATGCGAGTGCCCCGGGATGGGGCCTTCCTGGTGCGGAAACGCAATGAGCCTAACTCATATGCCATCTCTTTCCGGGCTGAGGGAAAGATCAAGCACTGCCGAGTACAGCAGGAAGGCCAGACAGTGATGCTGGGGAACTCTGAGTTTGACAGCCTGGTTGACCTCATCAGCTACTATGAGAAGCACCCCCTGTACCGCAAAATGAAGCTACGCTACCCCATCAACGAGGAGGCACTGGAGAAGATCGGGACAGCTGAACCCGATTATGGGGCACTATACGAGGGCCGCAACCCTGGTTTCTATGTGGAGGCAAACCCTATGCCAACTTTCAAGTGTGCAGTAAAAGCCCTCTTCGACTACAAGGCCCAGAGAGAGGATGAGCTGACCTTCACCAAGAGTGCCATCATCCAGAATGTGGAAAAGCAAGATGGTGGCTGGTGGCGAGGGGACTATGGTGGGAAGAAGCAGCTGTGGTTCCCCTCAAACTATGTGGAAGAGATGATCAATCCAGCAGTCCTAGAGCCTGAGAGGGAGCACCTGGATGAGAACAGCCCACTGGGGGACTTGCTGCGAGGGGTCTTAGATGTGCCAGCTTGTCAGATCGCCATCCGTCCTGAGGGCAAAAACAACCGGCTCTTCGTCTTCTCCATCAGCATGCCATCAGTGGCTCAGTGGTCCCTGGATGTTGCAGCTGACTCACAGGAGGAGTTACAGGACTGGGTGAAAAAGATCCGTGAAGTTGCCCAGACTGCAGATGCCAGGCTCACTGAGGGAAAGATGATGGAGAGGAGGAAGAAGATCGCCTTGGAGCTCTCCGAGCTTGTGGTCTACTGCCGGCCCGTTCCCTTTGATGAAGAGAAGATTGGCACAGAACGTGCTTGTTACCGGGACATGTCCTCCTTTCCGGAAACCAAGGCTGAGAAGTATGTGAACAAGGCCAAAGGCAAGAAGTTCCTCCAGTACAACCGGCTGCAGCTCTCGCGCATCTACCCTAAGGGCCAGAGGCTAGACTCCTCCAATTATGACCCTCTGCCCATGTGGATCTGCGGTAGCCAGCTTGTAGCACTCAATTTCCAGACCCCAGACAAGCCTATGCAGATGAACCAGGCCCTCTTCATGGCTGGTGGGCATTGTGGCTATGTGCTGCAGCCAAGCACCATGAGAGACGAAGCCTTTGACCCCTTTGATAAGAGCAGTCTCCGAGGTCTGGAACCCTGTGTCATTTGCATTGAGGTGCTGGGGGCCAGGCATCTGCCGAAGAATGGCCGGGGTATTGTGTGTCCTTTTGTGGAGATTGAGGTGGCTGGGGCTGAGTACGACAGCACCAAGCAAAAGACGGAGTTTGTAGTGGACAACGGACTGAACCCTGTGTGGCCTGCTAAGCCCTTCCACTTCCAGATCAGTAACCCAGAGTTTGCCTTTCTGCGCTTTGTGGTGTATGAGGAAGACATGTTTAGTGACCAGAACTTCTTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATATAGAGCAGTGCCTTTGAAGAACAACTACAGTGAAGACCTGGAGTTGGCCTCCCTGCTCATCAAGATTGACATTTTCCCTGCTAAGGAGAACGGTGACCTCAGTCCTTTCAGTGGCATATCCCTAAGGGAACGGGCCTCAGATGCCTCCAGCCAGCTGTTCCATGTCCGGGCCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGATTTCCGCATCTCGCAGGAGCATCTAGCAGACCATTTTGACAGTCGGGAACGAAGTTGA
Peptide sequence:
MRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLWFPSNYVEEMINPAVLEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMPSVAQWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGISLRERASDASSQLFHVRAREGSFEARYQQPFEDFRISQEHLADHFDSRERS
IRESfinder result
IRESfinder score
0.433465417
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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