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mmu_circPlcb4_115
Basic information of circular RNA
PanCircBase ID:
mmu_circPlcb4_115
mm39_Chr. Position ID:
chr2|135583749|135647525|+
Gene name:
Plcb4
Transcript:
ENSMUST00000137969.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
1,2
Exon/intron offsets:
0,63719
Genomic length:
63776
Exon/intron sizes in nt:
58,57
Flanking exon/introns:
None|chr2:135647525-135719302
Splice length:
115
Other database IDs:
circAtlas: mmu-Plcb4_0031
Human Ortholog IDs:
Mature spliced Sequence:
GAGCCTGCTTTGTGCGTGCTGTCAACTACCTGGGAGGATTCTCGACACAGCAGCCTGGGGACTGTGCAGGATTTGATCGGGTAGACCTCCCTTGCTTGCTGCTCCCAGCTGCCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGTAGACCTCCCTTGCTTG
59.75
TGTCGAGAATCCTCCCAGGT
59.96
84
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGTAGACCTCCCTTGCTTG
59.75
GCTGCTGTGTCGAGAATCCT
60.11
91
PCR template sequence:
GGACTGTGCAGGATTTGATCGGGTAGACCTCCCTTGCTTGCTGCTCCCAGCTGCCTGGAGCCTGCTTTGTGCGTGCTGTCAACTACCTGGGAGGATTCTCGACACAGCAGCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-15b-5p(1), mmu-miR-214-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCCAGCTGCCTGGAGCCTGCTTTGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.817016153
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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