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mmu_circPik3c3_529
Basic information of circular RNA
PanCircBase ID:
mmu_circPik3c3_529
mm39_Chr. Position ID:
chr18|30410024|30426183|+
Gene name:
Pik3c3
Transcript:
ENSMUST00000115812.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,4985,11779,13499,16087
Genomic length:
16159
Exon/intron sizes in nt:
144,130,87,96,72
Flanking exon/introns:
chr18:30407510-30410024|chr18:30426183-30426671
Splice length:
529
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTGGAACGAGTGGCTGAAACTTCCGGTGAAGTACCCTGACCTGCCCAGGAATGCCCAAGTGGCCTTGACTATATGGGATGTGTATGGACCGGGAAGCGCTGTGCCAGTGGGAGGAACAACCGTGTCGCTCTTTGGAAAATATGGCATGTTTCGCCAAGGAATGCATGACTTGAAGGTCTGGCCTAACGTGGAGGCAGATGGTTCTGAACCTACAAGAACTCCCGGCAGAACAAGCAGCACACTGTCAGAAGATCAGATGAGCCGCCTCGCCAAGCTCACCAAGGCTCATCGGCAAGGACACATGGTGAAAGTGGATTGGCTGGACAGATTAACATTTAGAGAGATAGAAATGATAAATGAGAGTGAGAAACGAAGCTCTAATTTCATGTACTTGATGGTTGAGTTTCGCTGTGTCAAGTGCGATGACAAGGAGTATGGCATTGTTTACTATGAAAAGGATGGTGACGAATCATCTCCAATTTTAACCAGTTTTGAGTTAGTGAAAGTTCCTGATCCTCAAATGTCTATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGATGGTGACGAATCATCTCCA
59.9
GCTTCCCGGTCCATACACAT
59.82
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGATGGTGACGAATCATCTCC
59.63
GCTTCCCGGTCCATACACAT
59.82
171
PCR template sequence:
GGAGTATGGCATTGTTTACTATGAAAAGGATGGTGACGAATCATCTCCAATTTTAACCAGTTTTGAGTTAGTGAAAGTTCCTGATCCTCAAATGTCTATGTTGGAACGAGTGGCTGAAACTTCCGGTGAAGTACCCTGACCTGCCCAGGAATGCCCAAGTGGCCTTGACTATATGGGATGTGTATGGACCGGGAAGCGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-34a-5p(1), mmu-miR-92a-3p(1), mmu-miR-30c-5p(1), mmu-miR-25-3p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-26b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAAATGTCTATGTTGGAACGAGTGG
siRNA sets
GTTCCAACATAGACATTTG, CACTCGTTCCAACATAGAC, ACTCGTTCCAACATAGACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPIK3C3_529_ORF_1
0.8212
0.210089504
0.542583423
423
ORF sequence:
ATGTTTCGCCAAGGAATGCATGACTTGAAGGTCTGGCCTAACGTGGAGGCAGATGGTTCTGAACCTACAAGAACTCCCGGCAGAACAAGCAGCACACTGTCAGAAGATCAGATGAGCCGCCTCGCCAAGCTCACCAAGGCTCATCGGCAAGGACACATGGTGAAAGTGGATTGGCTGGACAGATTAACATTTAGAGAGATAGAAATGATAAATGAGAGTGAGAAACGAAGCTCTAATTTCATGTACTTGATGGTTGAGTTTCGCTGTGTCAAGTGCGATGACAAGGAGTATGGCATTGTTTACTATGAAAAGGATGGTGACGAATCATCTCCAATTTTAACCAGTTTTGAGTTAGTGAAAGTTCCTGATCCTCAAATGTCTATGTTGGAACGAGTGGCTGAAACTTCCGGTGAAGTACCCTGA
Peptide sequence:
MFRQGMHDLKVWPNVEADGSEPTRTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMLERVAETSGEVP
IRESfinder result
IRESfinder score
0.377953394
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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