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mmu_circPign_2208
Basic information of circular RNA
PanCircBase ID:
mmu_circPign_2208
mm39_Chr. Position ID:
chr1|105493872|105585575|-
Gene name:
Pign
Transcript:
ENSMUST00000070699.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
20
Exon/intron indexes:
21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,13774,18841,21832,22901,23078,25474,31520,32901,71362,72035,74517,78331,80550,81961,83135,86956,89786,90518,91454
Genomic length:
91703
Exon/intron sizes in nt:
1,03,10,91,09,92,93,10,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:105485815-105493872|chr1:105585575-105586611
Splice length:
2208
Other database IDs:
circAtlas: mmu-Pign_0024
Human Ortholog IDs:
Mature spliced Sequence:
CTTCTCTTGCAGGTAGCCCAGTGTGGATATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATTTTACATCTCCTTTGGTTCATGGAATGACTCCCCAGTTTACACCTTTGCCTCCTCCAGCAAAAAGATTAGTGCTATTTGTTGCTGATGGCCTGAGAGCAGATACTCTTTATGAATTAGATGAAGATGGAAACTCCAGAGCACCTTTCATTAGGAATGTTATAATGCATGAAGGCAGCTGGGGAGTATCTCACACACGTGTGCCAACAGAATCTCGGCCTGGCCATGTAGCCTTGATAGCTGGATTTTATGAGGATGTCAGTGCAGTTGCCAAAGGATGGAAAGAAAATCCTGTAGAATTTGACTCTCTTTTTAATGAAAGCAAGTACACGTGGAGCTGGGGGAGTCCAGATATCTTGCCCATGTTTGCCAAAGGTGCTAGTGGTGACCATGTCTATACATATAGTTACGATGCTCAAAGAGAAGATTTTGGTGCCCACGATGCCACAAAACTGGATACGTGGGTTTTTGATAAAGTTAAGGACTTCTTTGATGCTGCAAGAAACAACCAATCTTTGTTCACAAAAGTAAATGAAGAAAAAGTAGTTTTTTTCTTACATTTATTGGGAATAGATACAAATGGACATGCTCATCGACCATCGTCAAGGGAATATAAGGACAATATTAAAAAAGTTGATGATGGAGTGAAGGAAATCGTATCAATTTTCAAGCATTTTTATGGAGATGATGGGAAAACAGCATTTATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCGTCAGAGACTTTAACTCCGTTCGTTACTTGGGGAGCTGGAATCAAATTTCCTCAAAATGTATCAGCTCAGCAATATGATGATGAGTTTTTAAAAGAATGGAGATTGGAAAACTGGAAAAGGAGAGATGTCAATCAGGCTGATATTGCACCTCTGATGGCATCCCTTATTGGAGTCCCCTTTCCTCTTAATTCGGTGGGAATACTTCCTGTGGGTTATCTTAACAACACTGGTCTCTTCAAAGCAGAGAGCATGTTTACCAATGCAGTACAGATTCTTGAACAGTTCAAGGTGAAAATGACTCAGAAAAAAGAAGCCACTTTACCGTTTTTGTTTACACCATTTAAGTTGCTTTCTGATTCTCAACAACTGGACATTTTAAGAAAAGCAAGATCTTACATAAAACAAGAAAAATTTGATGAAGTAGTGTCCCTCTGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGGGCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTCTAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCTACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGCTGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATTGACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTCAGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGCTGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACTGATGCCTGTCGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTGTCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTCTGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAAAGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTCCTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCCTAAGCACTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCTCTCTCTCAGCGACTG
59.9
AGATGGAAGCGAAGAACACA
57.16
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACTGGCCAGCATACTCCTC
59.89
AGATGGAAGCGAAGAACACA
57.16
141
PCR template sequence:
CTTCTCTGGTGGCACCACTCCTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCCTAAGCACTGGCTTCTCTTGCAGGTAGCCCAGTGTGGATATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
33
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(2), mmu-miR-107-3p(2), mmu-miR-3065-5p(2), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-199a-5p(1), mmu-miR-221-3p(1), mmu-miR-30e-5p(1), mmu-miR-31-5p(1), mmu-miR-200c-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-29b-3p(1), mmu-miR-146a-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-18a-5p(1), mmu-miR-222-3p(1), mmu-miR-132-3p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-136-5p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-340-5p(1), mmu-miR-383-5p(1), mmu-miR-342-5p(1), mmu-miR-466d-3p(1), mmu-miR-540-3p(1), mmu-miR-146b-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCTAAGCACTGGCTTCTCTTGCAGG
siRNA sets
CTGCAAGAGAAGCCAGTGC, TGCAAGAGAAGCCAGTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPIGN_2208_ORF_1
0.834
-0.010204877
0.999993917
2196
ORF sequence:
ATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATTTTACATCTCCTTTGGTTCATGGAATGACTCCCCAGTTTACACCTTTGCCTCCTCCAGCAAAAAGATTAGTGCTATTTGTTGCTGATGGCCTGAGAGCAGATACTCTTTATGAATTAGATGAAGATGGAAACTCCAGAGCACCTTTCATTAGGAATGTTATAATGCATGAAGGCAGCTGGGGAGTATCTCACACACGTGTGCCAACAGAATCTCGGCCTGGCCATGTAGCCTTGATAGCTGGATTTTATGAGGATGTCAGTGCAGTTGCCAAAGGATGGAAAGAAAATCCTGTAGAATTTGACTCTCTTTTTAATGAAAGCAAGTACACGTGGAGCTGGGGGAGTCCAGATATCTTGCCCATGTTTGCCAAAGGTGCTAGTGGTGACCATGTCTATACATATAGTTACGATGCTCAAAGAGAAGATTTTGGTGCCCACGATGCCACAAAACTGGATACGTGGGTTTTTGATAAAGTTAAGGACTTCTTTGATGCTGCAAGAAACAACCAATCTTTGTTCACAAAAGTAAATGAAGAAAAAGTAGTTTTTTTCTTACATTTATTGGGAATAGATACAAATGGACATGCTCATCGACCATCGTCAAGGGAATATAAGGACAATATTAAAAAAGTTGATGATGGAGTGAAGGAAATCGTATCAATTTTCAAGCATTTTTATGGAGATGATGGGAAAACAGCATTTATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCGTCAGAGACTTTAACTCCGTTCGTTACTTGGGGAGCTGGAATCAAATTTCCTCAAAATGTATCAGCTCAGCAATATGATGATGAGTTTTTAAAAGAATGGAGATTGGAAAACTGGAAAAGGAGAGATGTCAATCAGGCTGATATTGCACCTCTGATGGCATCCCTTATTGGAGTCCCCTTTCCTCTTAATTCGGTGGGAATACTTCCTGTGGGTTATCTTAACAACACTGGTCTCTTCAAAGCAGAGAGCATGTTTACCAATGCAGTACAGATTCTTGAACAGTTCAAGGTGAAAATGACTCAGAAAAAAGAAGCCACTTTACCGTTTTTGTTTACACCATTTAAGTTGCTTTCTGATTCTCAACAACTGGACATTTTAAGAAAAGCAAGATCTTACATAAAACAAGAAAAATTTGATGAAGTAGTGTCCCTCTGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGGGCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTCTAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCTACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGCTGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATTGACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTCAGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGCTGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACTGATGCCTGTCGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTGTCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTCTGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAAAGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTCCTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCCTAAGCACTGGCTTCTCTTGCAGGTAG
Peptide sequence:
MLLFFALGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPLNSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSLCEELIDLALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIATGVLVACFLMIQACPWTYYVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVLSFFYRYMLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAGFLVLLLSLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPLMNQIVSWATLASSLVAPLLSSTALSQRLASILLSLMSTYLLLSTGFSCR
IRESfinder result
IRESfinder score
0.741207687
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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