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mmu_circPign_1325
Basic information of circular RNA
PanCircBase ID:
mmu_circPign_1325
mm39_Chr. Position ID:
chr1|105480096|105525575|-
Gene name:
Pign
Transcript:
ENSMUST00000070699.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
26,25,24,23,22,21,20,19,18,17,16,15,14
Exon/intron offsets:
0,1692,2072,5091,5616,13776,27550,32617,35608,36677,36854,39250,45296
Genomic length:
45479
Exon/intron sizes in nt:
74,76,56,87,10,31,03,10,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:105476832-105480096|chr1:105525575-105526773
Splice length:
1325
Other database IDs:
CIRCpedia: MMU_CIRCpedia_221343, circAtlas: mmu-Pign_0030
Human Ortholog IDs:
Mature spliced Sequence:
GTGTCCCTCTGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGGGCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTCTAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCTACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGCTGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATTGACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTCAGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGCTGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACTGATGCCTGTCGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTGTCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTCTGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAAAGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTCCTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCCTAAGCACTGGGTATGAAGCTCTCTTTCCTTTAGTGCTGTCCTGTTTGATGTTTGTCTGGATCCAAGTGGAACAAGAGACCCTTCAACAGCCTGGTGTTTCTTGTAAACAGAAGCTCACCAGCATCCAGTTCACCTGTGACACTGATATAGCTCAGTTCCGACAGCTCTGTCCGGATGACATCCGTAGGGCCTTTTTCCTAGTTTTCTTTTTATTAACTGCATTTTTTGGAACTGGAAATATAGCTTCTATTAACAGCTTCGACCTTGCCTCTGTGTATTGCTTTCTGACTGTGTTTAGTCCTTTCATGATGGGAGCGCTCATGATGTGGAAGATTTTAATTCCTTTTGTTCTTGTGATGTGTGCTTTTGAAGCAGTTCAGATAACTACACAGCTGTCCTCAAAAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGGGAGCGCTCATGATG
60.25
CTCTCAGGGCGAGGTCAATC
59.9
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGGGAGCGCTCATGATG
60.25
GACAATCCTCTCAGGGCGAG
59.9
147
PCR template sequence:
TGATGGGAGCGCTCATGATGTGGAAGATTTTAATTCCTTTTGTTCTTGTGATGTGTGCTTTTGAAGCAGTTCAGATAACTACACAGCTGTCCTCAAAAGGGTGTCCCTCTGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGGGCATCAATGTTGCAGTTGGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-200c-3p(1), mmu-miR-29b-3p(1), mmu-miR-335-5p(1), mmu-miR-146a-5p(1), mmu-miR-132-3p(1), mmu-miR-127-5p(1), mmu-miR-340-5p(1), mmu-miR-466d-3p(1), mmu-miR-3065-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTCCTCAAAAGGGTGTCCCTCTGTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPIGN_1325_ORF_1
0.8806
-0.00468843
0.994689656
1236
ORF sequence:
ATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTCTAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCTACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGCTGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATTGACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTCAGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGCTGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACTGATGCCTGTCGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTGTCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTCTGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAAAGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTCCTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCCTAAGCACTGGGTATGAAGCTCTCTTTCCTTTAGTGCTGTCCTGTTTGATGTTTGTCTGGATCCAAGTGGAACAAGAGACCCTTCAACAGCCTGGTGTTTCTTGTAAACAGAAGCTCACCAGCATCCAGTTCACCTGTGACACTGATATAGCTCAGTTCCGACAGCTCTGTCCGGATGACATCCGTAGGGCCTTTTTCCTAGTTTTCTTTTTATTAACTGCATTTTTTGGAACTGGAAATATAGCTTCTATTAACAGCTTCGACCTTGCCTCTGTGTATTGCTTTCTGACTGTGTTTAGTCCTTTCATGATGGGAGCGCTCATGATGTGGAAGATTTTAATTCCTTTTGTTCTTGTGATGTGTGCTTTTGAAGCAGTTCAGATAACTACACAGCTGTCCTCAAAAGGGTGTCCCTCTGTGAGGAGCTGA
Peptide sequence:
MSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIATGVLVACFLMIQACPWTYYVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVLSFFYRYMLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAGFLVLLLSLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPLMNQIVSWATLASSLVAPLLSSTALSQRLASILLSLMSTYLLLSTGYEALFPLVLSCLMFVWIQVEQETLQQPGVSCKQKLTSIQFTCDTDIAQFRQLCPDDIRRAFFLVFFLLTAFFGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFVLVMCAFEAVQITTQLSSKGCPSVRS
IRESfinder result
IRESfinder score
0.813460374
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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