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mmu_circPias2_1102
Basic information of circular RNA
PanCircBase ID:
mmu_circPias2_1102
mm39_Chr. Position ID:
chr18|77207607|77237794|+
Gene name:
Pias2
Transcript:
ENSMUST00000114777.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
4,5,6,7,8,9,10,11,12,13
Exon/intron offsets:
0,144,6945,9044,10138,13179,14022,15868,25091,30149
Genomic length:
30187
Exon/intron sizes in nt:
51,91,135,106,74,161,134,172,140,38
Flanking exon/introns:
chr18:77193585-77207607|chr18:77237794-77240310
Splice length:
1102
Other database IDs:
circAtlas: mmu-Pias2_0018
circAtlas: hsa-PIAS2_0008
Human Ortholog IDs:
Mature spliced Sequence:
GGACTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCGACTTTGCTTGGCAGAGACCAGTTGCCCTCAAGAAGATAACTATCCCAATAGTTTGTGTATAAAAGTCAATGGGAAACTCTTTCCTTTGCCTGGCTATGCGCCACCACCTAAAAATGGGATTGAACAGAAGCGCCCTGGACGCCCCCTGAACATTACGTCTTTAGTTAGGTTGTCTTCAGCTGTGCCAAATCAGATTTCTATTTCTTGGGCATCTGAAATTGGAAAGAATTACTCCATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGTTATTACAGAGATTAAAAATGAAAGGTATTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTGCAGACCCTGATAGTGAAATTGCTACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAAATGAGGCTGACAATCCCGTGCCGTGCAGTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATCTTCAGATGAATGAGAAAAAGCCCACCTGGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCTGATACTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAAGATGGTTCTTGGTGTCCCATGAGACCTAAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAGTAGAAAGTTCAAGTGTCTTTAGTAAACCTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGATAGATGTTATTGATCTAACAATAGAGAGCTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGCATCTTTATGTCAGAAACACAAAGCAGTCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCCAGTGTGACTTCAGTTGATCCTGCTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCACCACACGCCAGTGTCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGATCCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTACCATTCCACCACACGC
60.32
TGGTCTCTGCCAAGCAAAGT
59.82
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTACCATTCCACCACACGC
60.32
ACTGGTCTCTGCCAAGCAAA
59.82
161
PCR template sequence:
AACAGACTACTCAGTACCATTCCACCACACGCCAGTGTCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGATCCCCAGGGACTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCGACTTTGCTTGGCAGAGACCAGTTGCCCTCAAGAAGATAACTATCCCAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-503-5p(1), mmu-miR-224-5p(1), mmu-miR-190b-5p(1), mmu-miR-335-3p(1), mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTTGATCCCCAGGGACTTTTTGCCG
siRNA sets
CGGCAAAAAGTCCCTGGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPIAS2_1102_ORF_1
0.8799
-0.014278626
0.943815734
906
ORF sequence:
ATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGTTATTACAGAGATTAAAAATGAAAGGTATTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTGCAGACCCTGATAGTGAAATTGCTACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAAATGAGGCTGACAATCCCGTGCCGTGCAGTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATCTTCAGATGAATGAGAAAAAGCCCACCTGGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCTGATACTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAAGATGGTTCTTGGTGTCCCATGAGACCTAAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAGTAGAAAGTTCAAGTGTCTTTAGTAAACCTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGATAGATGTTATTGATCTAACAATAGAGAGCTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGCATCTTTATGTCAGAAACACAAAGCAGTCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCCAGTGTGACTTCAGTTGATCCTGCTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCACCACACGCCAGTGTCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGATCCCCAGGGACTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCGACTTTGCTTGGCAGAGACCAGTTGCCCTCAAGAAGATAA
Peptide sequence:
MSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVTSQPCTKVESSSVFSKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGLDFLSLIPVDPQGLFAGWQERLHSPSPAATLLGRDQLPSRR
IRESfinder result
IRESfinder score
0.727261969
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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