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mmu_circPi4ka_489
Basic information of circular RNA
PanCircBase ID:
mmu_circPi4ka_489
mm39_Chr. Position ID:
chr16|17191213|17199753|-
Gene name:
Pi4ka
Transcript:
ENSMUST00000036161.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,6,5,4
Exon/intron offsets:
0,3568,5092,8451
Genomic length:
8540
Exon/intron sizes in nt:
67,260,73,89
Flanking exon/introns:
chr16:17185416-17191213|chr16:17199753-17204099
Splice length:
489
Other database IDs:
circAtlas: mmu-Pi4ka_0032
Human Ortholog IDs:
Mature spliced Sequence:
GTAACCTCCCAGTTGCAGAGAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCACTTAGGGATGAGATTTTAGAGGCAATTTTGCAGGTTTTACATGTCCTTTTGGGAATGTGCCAGGCCTTGGAGATTCAAGAAAAAGAATACCTTTGCAAGTATGCGATCCCATGCCTGATAGGAATCTCAAGATCTTTTGGACGTTACAGCAATTCAGAAGAGTCTCTCCTCTCAAAGCTTTTCCCCAAAGTTTCTCCTCATTCCCTGAGAATCCCAGAAGAGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTTCGCTCCATCCTGCCCAGCAACCTGCTAACTGTCTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGTGGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGTGTGTCCAGCATCTCT
60.04
AGCCACATCAGACAGCAAGG
60.32
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGTGTGTCCAGCATCTC
60.04
AGCCACATCAGACAGCAAGG
60.32
153
PCR template sequence:
AGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGTGGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAAGGTAACCTCCCAGTTGCAGAGAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCACTTAGGGATGAGATTTTAGAGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
CACTATTTTGAAGGTAACCTCCCAGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPI4KA_489_ORF_1
0.5278
0.11536001
0.908226997
846
ORF sequence:
ATGTGCCAGGCCTTGGAGATTCAAGAAAAAGAATACCTTTGCAAGTATGCGATCCCATGCCTGATAGGAATCTCAAGATCTTTTGGACGTTACAGCAATTCAGAAGAGTCTCTCCTCTCAAAGCTTTTCCCCAAAGTTTCTCCTCATTCCCTGAGAATCCCAGAAGAGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTTCGCTCCATCCTGCCCAGCAACCTGCTAACTGTCTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGTGGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAAGGTAACCTCCCAGTTGCAGAGAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCACTTAGGGATGAGATTTTAGAGGCAATTTTGCAGGTTTTACATGTCCTTTTGGGAATGTGCCAGGCCTTGGAGATTCAAGAAAAAGAATACCTTTGCAAGTATGCGATCCCATGCCTGATAGGAATCTCAAGATCTTTTGGACGTTACAGCAATTCAGAAGAGTCTCTCCTCTCAAAGCTTTTCCCCAAAGTTTCTCCTCATTCCCTGAGAATCCCAGAAGAGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTTCGCTCCATCCTGCCCAGCAACCTGCTAACTGTCTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGTGGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAA
Peptide sequence:
MCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFE
IRESfinder result
IRESfinder score
0.738544595
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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