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mmu_circPhlpp1_323
Basic information of circular RNA
PanCircBase ID:
mmu_circPhlpp1_323
mm39_Chr. Position ID:
chr1|106209092|106214846|+
Gene name:
Phlpp1
Transcript:
ENSMUST00000061047.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,5628
Genomic length:
5754
Exon/intron sizes in nt:
197,126
Flanking exon/introns:
chr1:106101177-106209092|chr1:106214846-106246578
Splice length:
323
Other database IDs:
circBase: mmu_circ_0008061, CIRCpedia: MMU_CIRCpedia_1740, circAtlas: mmu-Phlpp1_0008
circBase: hsa_circ_0108767, circAtlas: hsa-PHLPP1_0008, CIRCpedia: HSA_CIRCpedia_24166
Human Ortholog IDs:
Mature spliced Sequence:
GAAAGCCTCATAGCACTGGGAGCTCTGAGCGGATTCAGCTCTCCGGAATGTACAATGTCCGAAAAGGCAAAATGCAGTTGCCAGTGAACCGATGGACAAGACGACAGGTCATTCTGTGCGGGACGTGCTTGATAGTGTCGTCCGTGAAAGACAGCGTGAGTGGGAAGATGCATGTCCTGCCCCTCATTGGTGGAAAAGTGGAGGAAGTGAAAAAGCACCAGCACTGTTTAGCGTTCAGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGATACTTTCACGGAGTACTTACGGTGGCTGCGGCAAGTCTCCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTTCGGGGCCTCAAAGC
60.75
CATCGGTTCACTGGCAACTG
59.48
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCTTCGGGGCCTCAAAG
60.75
CATCGGTTCACTGGCAACTG
59.48
180
PCR template sequence:
CTGTTTAGCGTTCAGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGATACTTTCACGGAGTACTTACGGTGGCTGCGGCAAGTCTCCAAGGAAAGCCTCATAGCACTGGGAGCTCTGAGCGGATTCAGCTCTCCGGAATGTACAATGTCCGAAAAGGCAAAATGCAGTTGCCAGTGAACCGATGGACAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-382-3p(1), mmu-miR-425-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAAGTCTCCAAGGAAAGCCTCATAG
siRNA sets
GCTTTCCTTGGAGACTTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPHLPP1_323_ORF_1
0.8975
0.206186424
0.468375674
363
ORF sequence:
ATGTACAATGTCCGAAAAGGCAAAATGCAGTTGCCAGTGAACCGATGGACAAGACGACAGGTCATTCTGTGCGGGACGTGCTTGATAGTGTCGTCCGTGAAAGACAGCGTGAGTGGGAAGATGCATGTCCTGCCCCTCATTGGTGGAAAAGTGGAGGAAGTGAAAAAGCACCAGCACTGTTTAGCGTTCAGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGATACTTTCACGGAGTACTTACGGTGGCTGCGGCAAGTCTCCAAGGAAAGCCTCATAGCACTGGGAGCTCTGAGCGGATTCAGCTCTCCGGAATGTACAATGTCCGAAAAGGCAAAATGCAGTTGCCAGTGA
Peptide sequence:
MYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSVSGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKESLIALGALSGFSSPECTMSEKAKCSCQ
IRESfinder result
IRESfinder score
0.386515662
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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