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mmu_circPhf21a_1176
Basic information of circular RNA
PanCircBase ID:
mmu_circPhf21a_1176
mm39_Chr. Position ID:
chr2|92158656|92189554|+
Gene name:
Phf21a
Transcript:
ENSMUST00000044036.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
6,7,8,9,10,11,12,13,14,15
Exon/intron offsets:
0,2203,16232,19684,20566,21076,23389,28552,30382,30794
Genomic length:
30898
Exon/intron sizes in nt:
9,02,94,99,52,80,61,16,00,00,00,000
Flanking exon/introns:
chr2:92150804-92158656|chr2:92189554-92190513
Splice length:
1176
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAGGTTCAAGCAACACCTCCTCAGCCCATCAAAGTACCACAGTTTATCCCTCCTCCTAGACTCACTCCACGCCCAAACTTCCTTCCACAGGTTCGACCCAAGCCTGTGGCCCAGAATAACATTCCTATCGCTCCAGCGCCTCCTCCCATGCTTGCAGCTCCTCAGCTTATCCAGAGGCCTGTCATGCTGACCAAGTTTACACCCACAACCCTCCCCACATCCCAGAATTCCATCCACCCTGTCCGTGTTGTCAATGGACAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCACCAGCATTGTCATAGCTACTCCAGGGACCAGACTCCCTGGACCTCAGACTGTACAGCTTAGCAAACCAAGCCTGGAGAAACAGACAGTTAAATCCCACCCAGAAGCAGAGGAAAAACAAGCAGAGAGCCGTACCGTTACCCCACCTGCTGCACCAAAACCAAAACGAGAAGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGTTGGTAACACATGACCATCTAGAAGAAATCCAAAGCAAGAGGCAGGAAAGAAAGAGAAGAACAACAGCAAACCCGGTGTACAGTGGGGCTGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTCACTTACCTTAACAGCACAATGCATCCTGGGACGCGGAAGAGAGGTCGTCCTCCAAAATACAATGCGGTGCTGGGGTTTGGAGCCCTTACCCCAACATCCCCCCCATCCAGTCATCCCGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTTCCCTGCACCTGTTCAGCCTGTGTCTTTGCCCAGCCCCACCTCCACAGACGGTGATATCCATGAGGATTTTTGCAGTGTTTGCAGAAAAAGTGGCCAGTTGCTGATGTGTGACACGTGTTCCCGTGTGTATCATCTGGACTGCTTAGAGCCGCCCCTGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCTAAAGAAGGAAGAAGCAATTCCTTGGCCTGGAACCTTAGCCATTGTTCATTCCTACATCGCCTACAAAGCAGCAAAAGAAGAGGAGAAACAGAAGCTACTTAAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAGAGCTCAGCAGTTCTATAAGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAACAGGAGCGGGAACAACT
59.82
GAAGGAAGTTTGGGCGTGGA
60.54
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAACAGGAGCGGGAACAACT
59.82
AAGGAAGTTTGGGCGTGGAG
60.54
141
PCR template sequence:
AGAAGAGGAGAAACAGAAGCTACTTAAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAGAGCTCAGCAGTTCTATAAGTCAGGTTCAAGCAACACCTCCTCAGCCCATCAAAGTACCACAGTTTATCCCTCCTCCTAGACTCACTCCACGCCCAAACTTCCTTCCACAGGTTCGACCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-19b-3p(1), mmu-miR-33-5p(1), mmu-miR-25-3p(1), mmu-miR-127-3p(1), mmu-miR-377-3p(1), mmu-miR-1198-5p(1), mmu-miR-1904(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGTTCTATAAGTCAGGTTCAAGCAA
siRNA sets
AACCTGACTTATAGAACTG, GAACCTGACTTATAGAACT, TTGAACCTGACTTATAGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPHF21A_1176_ORF_1
0.9496
0.173283291
0.999997931
2205
ORF sequence:
ATGCTTGCAGCTCCTCAGCTTATCCAGAGGCCTGTCATGCTGACCAAGTTTACACCCACAACCCTCCCCACATCCCAGAATTCCATCCACCCTGTCCGTGTTGTCAATGGACAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCACCAGCATTGTCATAGCTACTCCAGGGACCAGACTCCCTGGACCTCAGACTGTACAGCTTAGCAAACCAAGCCTGGAGAAACAGACAGTTAAATCCCACCCAGAAGCAGAGGAAAAACAAGCAGAGAGCCGTACCGTTACCCCACCTGCTGCACCAAAACCAAAACGAGAAGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGTTGGTAACACATGACCATCTAGAAGAAATCCAAAGCAAGAGGCAGGAAAGAAAGAGAAGAACAACAGCAAACCCGGTGTACAGTGGGGCTGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTCACTTACCTTAACAGCACAATGCATCCTGGGACGCGGAAGAGAGGTCGTCCTCCAAAATACAATGCGGTGCTGGGGTTTGGAGCCCTTACCCCAACATCCCCCCCATCCAGTCATCCCGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTTCCCTGCACCTGTTCAGCCTGTGTCTTTGCCCAGCCCCACCTCCACAGACGGTGATATCCATGAGGATTTTTGCAGTGTTTGCAGAAAAAGTGGCCAGTTGCTGATGTGTGACACGTGTTCCCGTGTGTATCATCTGGACTGCTTAGAGCCGCCCCTGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCTAAAGAAGGAAGAAGCAATTCCTTGGCCTGGAACCTTAGCCATTGTTCATTCCTACATCGCCTACAAAGCAGCAAAAGAAGAGGAGAAACAGAAGCTACTTAAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAGAGCTCAGCAGTTCTATAAGTCAGGTTCAAGCAACACCTCCTCAGCCCATCAAAGTACCACAGTTTATCCCTCCTCCTAGACTCACTCCACGCCCAAACTTCCTTCCACAGGTTCGACCCAAGCCTGTGGCCCAGAATAACATTCCTATCGCTCCAGCGCCTCCTCCCATGCTTGCAGCTCCTCAGCTTATCCAGAGGCCTGTCATGCTGACCAAGTTTACACCCACAACCCTCCCCACATCCCAGAATTCCATCCACCCTGTCCGTGTTGTCAATGGACAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCACCAGCATTGTCATAGCTACTCCAGGGACCAGACTCCCTGGACCTCAGACTGTACAGCTTAGCAAACCAAGCCTGGAGAAACAGACAGTTAAATCCCACCCAGAAGCAGAGGAAAAACAAGCAGAGAGCCGTACCGTTACCCCACCTGCTGCACCAAAACCAAAACGAGAAGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGTTGGTAACACATGACCATCTAGAAGAAATCCAAAGCAAGAGGCAGGAAAGAAAGAGAAGAACAACAGCAAACCCGGTGTACAGTGGGGCTGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTCACTTACCTTAACAGCACAATGCATCCTGGGACGCGGAAGAGAGGTCGTCCTCCAAAATACAATGCGGTGCTGGGGTTTGGAGCCCTTACCCCAACATCCCCCCCATCCAGTCATCCCGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTTCCCTGCACCTGTTCAGCCTGTGTCTTTGCCCAGCCCCACCTCCACAGACGGTGATATCCATGAGGATTTTTGCAGTGTTTGCAGAAAAAGTGGCCAGTTGCTGATGTGTGACACGTGTTCCCGTGTGTATCATCTGGACTGCTTAGAGCCGCCCCTGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCTAAAGAAGGAAGAAGCAATTCCTTGGCCTGGAACCTTAGCCATTGTTCATTCCTACATCGCCTACAAAGCAGCAAAAGAAGAGGAGAAACAGAAGCTACTTAAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAGAGCTCAGCAGTTCTATAAGT
Peptide sequence:
MLAAPQLIQRPVMLTKFTPTTLPTSQNSIHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLPGPQTVQLSKPSLEKQTVKSHPEAEEKQAESRTVTPPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSTMHPGTRKRGRPPKYNAVLGFGALTPTSPPSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLEPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLLKWSSDLKQEREQLEQKVKELSSSISQVQATPPQPIKVPQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQNSIHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLPGPQTVQLSKPSLEKQTVKSHPEAEEKQAESRTVTPPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSTMHPGTRKRGRPPKYNAVLGFGALTPTSPPSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLEPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLLKWSSDLKQEREQLEQKVKELSSSIS
IRESfinder result
IRESfinder score
0.639704749
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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