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mmu_circPde10a_391
Basic information of circular RNA
PanCircBase ID:
mmu_circPde10a_391
mm39_Chr. Position ID:
chr17|9181719|9186406|+
Gene name:
Pde10a
Transcript:
ENSMUST00000115715.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
15,16,17
Exon/intron offsets:
0,1677,4531
Genomic length:
4687
Exon/intron sizes in nt:
8,41,51,156
Flanking exon/introns:
chr17:9180834-9181719|chr17:9186406-9188359
Splice length:
391
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATTCCACTTTGACATTGGTCCTTTCGAGAACATGTGGCCTGGGATCTTTGTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAAAAATTGTGCCGTTTTATCATGTCTGTGAAGAAGAACTATCGGCGGGTTCCTTACCACAACTGGAAGCATGCAGTCACGGTGGCACACTGCATGTATGCCATACTTCAAAACAACAATGGCCTCTTCACAGACCTCGAGCGCAAAGGCCTGCTAATTGCGTGTCTGTGCCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAGTTCGACCACCCCCTGGCGGCGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGGTGTCCATCCTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTGCAGAAGTTCGACCAC
60.25
GACAAAGATCCCAGGCCACA
59.96
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTGCAGAAGTTCGACCAC
60.25
ATGTCCCACAAGACCGATGG
59.75
172
PCR template sequence:
TAACAGCTACCTGCAGAAGTTCGACCACCCCCTGGCGGCGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGGTGTCCATCCTTCAGATTCCACTTTGACATTGGTCCTTTCGAGAACATGTGGCCTGGGATCTTTGTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCCATCCTTCAGATTCCACTTTGAC
siRNA sets
AGTGGAATCTGAAGGATGG, GTGGAATCTGAAGGATGGA, AAGTGGAATCTGAAGGATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPDE10A_391_ORF_1
0.7258
0.211708811
0.663184232
516
ORF sequence:
ATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAGTTCGACCACCCCCTGGCGGCGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGGTGTCCATCCTTCAGATTCCACTTTGACATTGGTCCTTTCGAGAACATGTGGCCTGGGATCTTTGTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAAAAATTGTGCCGTTTTATCATGTCTGTGAAGAAGAACTATCGGCGGGTTCCTTACCACAACTGGAAGCATGCAGTCACGGTGGCACACTGCATGTATGCCATACTTCAAAACAACAATGGCCTCTTCACAGACCTCGAGCGCAAAGGCCTGCTAATTGCGTGTCTGTGCCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAGTTCGACCACCCCCTGGCGGCGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGGTGTCCATCCTTCAG
Peptide sequence:
MTWTTGASVTATCRSSTTPWRRCTPPPPWSNTTSPRRCPSFRFHFDIGPFENMWPGIFVYMIHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNNGLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQ
IRESfinder result
IRESfinder score
0.563575303
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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