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mmu_circP2rx7_439
Basic information of circular RNA
PanCircBase ID:
mmu_circP2rx7_439
mm39_Chr. Position ID:
chr5|122801639|122808591|+
Gene name:
P2rx7
Transcript:
ENSMUST00000100737.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
6,7,8,9
Exon/intron offsets:
0,968,2567,6861
Genomic length:
6952
Exon/intron sizes in nt:
81,130,137,91
Flanking exon/introns:
chr5:122796867-122801639|chr5:122808591-122811417
Splice length:
439
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACCTGCACTCTTGAGGAGCGCCGAAAACTTCACCGTACTCATCAAGAATAATATCCACTTCCCCGGCCACAACTATACCACGAGAAACATCTTGCCAACTATGAACGGCTCTTGTACCTTTCACAAGACTTGGGACCCTCAGTGTTCCATCTTCCGACTAGGGGACATCTTCCAGGAAGCAGGAGAGAACTTTACAGAGGTGGCAGTTCAGGGAGGAATCATGGGTATCGAGATCTACTGGGATTGCAACCTAGACAGCTGGTCCCATCACTGCCGACCCAGGTACAGCTTCCGCCGCCTGGATGACAAGAACACGGATGAGTCCTTCGTCCCCGGCTACAACTTCAGATATGCCAAGTACTATAAGGAGAACAATGTGGAAAAGCGGACGTTGATCAAAGCCTTCGGCATCCGTTTTGACATCCTGGTTTTCGGCACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGGAAAAGCGGACGTTGA
60.46
TCGTGGTATAGTTGTGGCCG
59.83
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCGGACGTTGATCAAAGC
59.48
TCGTGGTATAGTTGTGGCCG
59.83
140
PCR template sequence:
CAACTTCAGATATGCCAAGTACTATAAGGAGAACAATGTGGAAAAGCGGACGTTGATCAAAGCCTTCGGCATCCGTTTTGACATCCTGGTTTTCGGCACTACCTGCACTCTTGAGGAGCGCCGAAAACTTCACCGTACTCATCAAGAATAATATCCACTTCCCCGGCCACAACTATACCACGAGAAACATCTTGCCAACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-31-5p(1), mmu-miR-34b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTTTTCGGCACTACCTGCACTCTTG
siRNA sets
CAAGAGTGCAGGTAGTGCC, AAGAGTGCAGGTAGTGCCG, AGAGTGCAGGTAGTGCCGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCP2RX7_439_ORF_1
0.7698
0.413708802
0.662336352
390
ORF sequence:
ATGAACGGCTCTTGTACCTTTCACAAGACTTGGGACCCTCAGTGTTCCATCTTCCGACTAGGGGACATCTTCCAGGAAGCAGGAGAGAACTTTACAGAGGTGGCAGTTCAGGGAGGAATCATGGGTATCGAGATCTACTGGGATTGCAACCTAGACAGCTGGTCCCATCACTGCCGACCCAGGTACAGCTTCCGCCGCCTGGATGACAAGAACACGGATGAGTCCTTCGTCCCCGGCTACAACTTCAGATATGCCAAGTACTATAAGGAGAACAATGTGGAAAAGCGGACGTTGATCAAAGCCTTCGGCATCCGTTTTGACATCCTGGTTTTCGGCACTACCTGCACTCTTGAGGAGCGCCGAAAACTTCACCGTACTCATCAAGAATAA
Peptide sequence:
MNGSCTFHKTWDPQCSIFRLGDIFQEAGENFTEVAVQGGIMGIEIYWDCNLDSWSHHCRPRYSFRRLDDKNTDESFVPGYNFRYAKYYKENNVEKRTLIKAFGIRFDILVFGTTCTLEERRKLHRTHQE
IRESfinder result
IRESfinder score
0.413280559
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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