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mmu_circP2rx4_1006
Basic information of circular RNA
PanCircBase ID:
mmu_circP2rx4_1006
mm39_Chr. Position ID:
chr5|122852461|122865903|+
Gene name:
P2rx4
Transcript:
ENSMUST00000031429.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11
Exon/intron offsets:
0,3914,4102,4731,10195,10591,10831,12799,13000,13346
Genomic length:
13442
Exon/intron sizes in nt:
148,72,73,97,81,142,137,94,66,96
Flanking exon/introns:
chr5:122845850-122852461|chr5:122865903-122866349
Splice length:
1006
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGGTGTTCGTGTGGGAAAAGGGCTACCAGGAAACGGACTCTGTGGTCAGCTCGGTGACAACCAAAGCCAAAGGTGTGGCTGTGACCAACACTTCTCAGCTTGGATTCCGGATCTGGGACGTGGCCGACTATGTGGTCCCAGCTCAGGAGGAAAACTCCCTCTTCATCATGACCAACATGATCGTCACCGTGAACCAGACGCAGGGTACCTGTCCAGAGATTCCTGATAAGACCAGCATTTGCGATTCAGACGCCAACTGCACTCTCGGCTCCTCGGACACCCACAGCAGTGGAATTGGGACTGGAAGGTGTGTTCCATTCAACGCGTCTGTGAAGACCTGTGAGGTGGCCGCATGGTGCCCAGTGGAGAATGACGCTGGTGTGCCAACGCCAGCTTTTTTAAAGGCTGCAGAAAACTTCACCCTCTTGGTAAAGAACAACATCTGGTACCCCAAGTTTAATTTCAGCAAGAGGAATATCCTCCCCAACATTACCACCTCCTACCTCAAGTCGTGCATTTATAATGCGCGGACGGATCCCTTCTGCCCCATATTCCGTCTTGGCCAAATCGTGGCGGATGCGGGCCACAGCTTTCAGGAGATGGCAGTGGAGGGAGGCATCATGGGTATCCAGATCAAGTGGGACTGCAACCTTGACAGAGCTGCCTCCCACTGCCTGCCCAGATATTCCTTCCGGCGCCTCGACACTCGGGACTTAGAACACAACGTGTCTCCTGGCTACAATTTCAGGTTTGCCAAGTACTACAGGGACCTTGCTGGCAATGAGCAACGCACACTCACCAAGGCATATGGCATCCGCTTTGACATCATCGTGTTTGGAAAGGCTGGCAAGTTTGACATCATCCCAACCATGATCAATGTTGGCTCTGGCTTGGCGCTCCTAGGGGTGGCGACCGTGCTGTGTGACGTCATAGTCCTCTACTGTATGAAGAAGAGATACTACTACCGGGACAAGAAATATAAGTATGTGGAAGACTACGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCGTGCTGTGTGACGTCATA
60.39
GCTGACCACAGAGTCCGTTT
60.25
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCGTGCTGTGTGACGTCATA
60.39
TGACCACAGAGTCCGTTTCC
59.61
142
PCR template sequence:
GGTGGCGACCGTGCTGTGTGACGTCATAGTCCTCTACTGTATGAAGAAGAGATACTACTACCGGGACAAGAAATATAAGTATGTGGAAGACTACGAGCAGGTGGGTGTTCGTGTGGGAAAAGGGCTACCAGGAAACGGACTCTGTGGTCAGCTCGGTGACAACCAAAGCCAAAGGTGTGGCTGTGACCAACACTTCTCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-34c-5p(1), mmu-let-7c-5p(1), mmu-miR-320-3p(1), mmu-miR-138-5p(1), mmu-let-7d-5p(1), mmu-miR-127-3p(1), mmu-miR-433-5p(1), mmu-miR-96-5p(1), mmu-miR-98-5p(1), mmu-miR-705(1), mmu-miR-673-5p(1), mmu-miR-532-5p(1), mmu-miR-574-5p(1), mmu-miR-339-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGACTACGAGCAGGTGGGTGTTCGTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCP2RX4_1006_ORF_1
0.8192
0.306895054
0.994366712
1065
ORF sequence:
ATGACCAACATGATCGTCACCGTGAACCAGACGCAGGGTACCTGTCCAGAGATTCCTGATAAGACCAGCATTTGCGATTCAGACGCCAACTGCACTCTCGGCTCCTCGGACACCCACAGCAGTGGAATTGGGACTGGAAGGTGTGTTCCATTCAACGCGTCTGTGAAGACCTGTGAGGTGGCCGCATGGTGCCCAGTGGAGAATGACGCTGGTGTGCCAACGCCAGCTTTTTTAAAGGCTGCAGAAAACTTCACCCTCTTGGTAAAGAACAACATCTGGTACCCCAAGTTTAATTTCAGCAAGAGGAATATCCTCCCCAACATTACCACCTCCTACCTCAAGTCGTGCATTTATAATGCGCGGACGGATCCCTTCTGCCCCATATTCCGTCTTGGCCAAATCGTGGCGGATGCGGGCCACAGCTTTCAGGAGATGGCAGTGGAGGGAGGCATCATGGGTATCCAGATCAAGTGGGACTGCAACCTTGACAGAGCTGCCTCCCACTGCCTGCCCAGATATTCCTTCCGGCGCCTCGACACTCGGGACTTAGAACACAACGTGTCTCCTGGCTACAATTTCAGGTTTGCCAAGTACTACAGGGACCTTGCTGGCAATGAGCAACGCACACTCACCAAGGCATATGGCATCCGCTTTGACATCATCGTGTTTGGAAAGGCTGGCAAGTTTGACATCATCCCAACCATGATCAATGTTGGCTCTGGCTTGGCGCTCCTAGGGGTGGCGACCGTGCTGTGTGACGTCATAGTCCTCTACTGTATGAAGAAGAGATACTACTACCGGGACAAGAAATATAAGTATGTGGAAGACTACGAGCAGGTGGGTGTTCGTGTGGGAAAAGGGCTACCAGGAAACGGACTCTGTGGTCAGCTCGGTGACAACCAAAGCCAAAGGTGTGGCTGTGACCAACACTTCTCAGCTTGGATTCCGGATCTGGGACGTGGCCGACTATGTGGTCCCAGCTCAGGAGGAAAACTCCCTCTTCATCATGACCAACATGATCGTCACCGTGAACCAGACGCAGGGTACCTGTCCAGAGATTCCTGA
Peptide sequence:
MTNMIVTVNQTQGTCPEIPDKTSICDSDANCTLGSSDTHSSGIGTGRCVPFNASVKTCEVAAWCPVENDAGVPTPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNITTSYLKSCIYNARTDPFCPIFRLGQIVADAGHSFQEMAVEGGIMGIQIKWDCNLDRAASHCLPRYSFRRLDTRDLEHNVSPGYNFRFAKYYRDLAGNEQRTLTKAYGIRFDIIVFGKAGKFDIIPTMINVGSGLALLGVATVLCDVIVLYCMKKRYYYRDKKYKYVEDYEQVGVRVGKGLPGNGLCGQLGDNQSQRCGCDQHFSAWIPDLGRGRLCGPSSGGKLPLHHDQHDRHREPDAGYLSRDS
IRESfinder result
IRESfinder score
0.442197699
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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