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mmu_circOsbpl8_1359
Basic information of circular RNA
PanCircBase ID:
mmu_circOsbpl8_1359
mm39_Chr. Position ID:
chr10|111103465|111118085|+
Gene name:
Osbpl8
Transcript:
ENSMUST00000105275.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
8,9,10,11,12,13,14,15,16,17
Exon/intron offsets:
0,1753,2886,4469,5421,7386,8855,9642,12685,14522
Genomic length:
14620
Exon/intron sizes in nt:
204,85,172,238,185,82,99,97,99,98
Flanking exon/introns:
chr10:111101497-111103465|chr10:111118085-111120190
Splice length:
1359
Other database IDs:
circAtlas: hsa-OSBPL8_0079
Human Ortholog IDs:
Mature spliced Sequence:
ATTCGTGGTACCCTGAAGAGCTGGACCAAGCTGTGGTGTGTGTTGAAACCTGGCGTGCTTCTGATCTATAAAACACAAAAAAATGGTCAGTGGGTCGGAACAGTCCTTCTGAATGCTTGTGAGATCATTGAACGTCCATCAAAAAAGGATGGCTTTTGTTTCAAGCTTTTCCATCCTTTGGAGCAGTCTATTTGGGCAGTGAAGGGTCCTAAAGGTGAAGCGGTTGGCTCCATAACCCAGCCTCTACCTAGCAGCTACTTGATTATCCGAGCTACTTCAGAGTCAGATGGAAGGTGCTGGATGGATGCTTTGGAGTTGGCTTTGAAGTGTTCCAGTCTCCTTAAGCGGACCATGGTGAGGGAGGGGAAGGAGCACGACCTGAGCATCTCCTCAGACAGCACACACGTGACGCTGTATGGCTTACTGCGCGCTAACAATCTCCACAGTGGCGACAACTTCCAGTTGAATGACAGTGAGATCGAAAGACAGCACTTTAAGGACCAAGATTTGTACTCTGATAAATCTGACAAGGAGAACGACCCGGAGCACGATGAGTCTGACAACGAGGTGCTGGGGAAGAGCGAGGAGAGCGACACAGACACGTCCGAAAGGCAAGACGACTCGTACATCGACCCTGAGCCTGTGGAGCCCTTAAAGGAGACCACCTACATGGAGCAGAGCCACGAAGAACTGGGAGAGGCAGGTGAGGCCTCTCAAACGGAAACTGTGTCTGAAGAAAACAAAAGCCTTATCTGGACGCTGCTCAAGCAAGTCCGTCCCGGCATGGACCTATCCAGGGTGGTTCTTCCTACGTTTATTTTGGAGCCCCGCTCTTTCCTGGATAAGCTTTCAGATTACTACTACCACGCCGATTTCCTGTCTGAAGCTGCTCTAGAAGAAAATCCGTATTTCCGTTTGAAGAAAGTCGTGAAATGGTATTTGTCAGGATTTTATAAGAAGCCAAAGGGACTAAAGAAACCATATAATCCTATACTTGGTGAGACTTTCCGATGTTTATGGATTCATCCCAGAACAAACAGCAAAACTTTCTATATTGCTGAACAGGTGTCCCATCACCCACCAATATCTGCCTTTTATGTCAGTAACCGGAAAGATGGGTTTTGCCTTAGTGGTAGCATCCTGGCTAAGTCCAAGTTTTATGGAAACTCGTTATCTGCCATATTGGAGGGAGAAGCCCGGTTAACTTTCTTGAACAGAGGTGAAGATTACGTCATGACAATGCCATATGCTCACTGTAAAGGAATTCTTTATGGCACAATGACCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCGATACTTGAATTTAAACTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAATGACCCTGGAGCTTGGT
59.96
CACGCCAGGTTTCAACACAC
60.25
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAATGACCCTGGAGCTTGG
59.96
CACGCCAGGTTTCAACACAC
60.25
141
PCR template sequence:
AGGAATTCTTTATGGCACAATGACCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCGATACTTGAATTTAAACTAAAGATTCGTGGTACCCTGAAGAGCTGGACCAAGCTGTGGTGTGTGTTGAAACCTGGCGTGCTTCTGATCTATAAAACACAAAAAAATGGTCAGTGGGTCGGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-93-5p(1), mmu-miR-26a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-106b-5p(1), mmu-miR-30c-5p(1), mmu-miR-25-3p(1), mmu-miR-433-5p(1), mmu-miR-363-3p(1), mmu-miR-26b-5p(1), mmu-miR-382-5p(1), mmu-miR-324-3p(1), mmu-miR-383-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-673-5p(1), mmu-miR-5101(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
ATTTAAACTAAAGATTCGTGGTACCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCOSBPL8_1359_ORF_1
0.9258
0.23400211
0.999999634
2418
ORF sequence:
ATGGATGCTTTGGAGTTGGCTTTGAAGTGTTCCAGTCTCCTTAAGCGGACCATGGTGAGGGAGGGGAAGGAGCACGACCTGAGCATCTCCTCAGACAGCACACACGTGACGCTGTATGGCTTACTGCGCGCTAACAATCTCCACAGTGGCGACAACTTCCAGTTGAATGACAGTGAGATCGAAAGACAGCACTTTAAGGACCAAGATTTGTACTCTGATAAATCTGACAAGGAGAACGACCCGGAGCACGATGAGTCTGACAACGAGGTGCTGGGGAAGAGCGAGGAGAGCGACACAGACACGTCCGAAAGGCAAGACGACTCGTACATCGACCCTGAGCCTGTGGAGCCCTTAAAGGAGACCACCTACATGGAGCAGAGCCACGAAGAACTGGGAGAGGCAGGTGAGGCCTCTCAAACGGAAACTGTGTCTGAAGAAAACAAAAGCCTTATCTGGACGCTGCTCAAGCAAGTCCGTCCCGGCATGGACCTATCCAGGGTGGTTCTTCCTACGTTTATTTTGGAGCCCCGCTCTTTCCTGGATAAGCTTTCAGATTACTACTACCACGCCGATTTCCTGTCTGAAGCTGCTCTAGAAGAAAATCCGTATTTCCGTTTGAAGAAAGTCGTGAAATGGTATTTGTCAGGATTTTATAAGAAGCCAAAGGGACTAAAGAAACCATATAATCCTATACTTGGTGAGACTTTCCGATGTTTATGGATTCATCCCAGAACAAACAGCAAAACTTTCTATATTGCTGAACAGGTGTCCCATCACCCACCAATATCTGCCTTTTATGTCAGTAACCGGAAAGATGGGTTTTGCCTTAGTGGTAGCATCCTGGCTAAGTCCAAGTTTTATGGAAACTCGTTATCTGCCATATTGGAGGGAGAAGCCCGGTTAACTTTCTTGAACAGAGGTGAAGATTACGTCATGACAATGCCATATGCTCACTGTAAAGGAATTCTTTATGGCACAATGACCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCGATACTTGAATTTAAACTAAAGATTCGTGGTACCCTGAAGAGCTGGACCAAGCTGTGGTGTGTGTTGAAACCTGGCGTGCTTCTGATCTATAAAACACAAAAAAATGGTCAGTGGGTCGGAACAGTCCTTCTGAATGCTTGTGAGATCATTGAACGTCCATCAAAAAAGGATGGCTTTTGTTTCAAGCTTTTCCATCCTTTGGAGCAGTCTATTTGGGCAGTGAAGGGTCCTAAAGGTGAAGCGGTTGGCTCCATAACCCAGCCTCTACCTAGCAGCTACTTGATTATCCGAGCTACTTCAGAGTCAGATGGAAGGTGCTGGATGGATGCTTTGGAGTTGGCTTTGAAGTGTTCCAGTCTCCTTAAGCGGACCATGGTGAGGGAGGGGAAGGAGCACGACCTGAGCATCTCCTCAGACAGCACACACGTGACGCTGTATGGCTTACTGCGCGCTAACAATCTCCACAGTGGCGACAACTTCCAGTTGAATGACAGTGAGATCGAAAGACAGCACTTTAAGGACCAAGATTTGTACTCTGATAAATCTGACAAGGAGAACGACCCGGAGCACGATGAGTCTGACAACGAGGTGCTGGGGAAGAGCGAGGAGAGCGACACAGACACGTCCGAAAGGCAAGACGACTCGTACATCGACCCTGAGCCTGTGGAGCCCTTAAAGGAGACCACCTACATGGAGCAGAGCCACGAAGAACTGGGAGAGGCAGGTGAGGCCTCTCAAACGGAAACTGTGTCTGAAGAAAACAAAAGCCTTATCTGGACGCTGCTCAAGCAAGTCCGTCCCGGCATGGACCTATCCAGGGTGGTTCTTCCTACGTTTATTTTGGAGCCCCGCTCTTTCCTGGATAAGCTTTCAGATTACTACTACCACGCCGATTTCCTGTCTGAAGCTGCTCTAGAAGAAAATCCGTATTTCCGTTTGAAGAAAGTCGTGAAATGGTATTTGTCAGGATTTTATAAGAAGCCAAAGGGACTAAAGAAACCATATAATCCTATACTTGGTGAGACTTTCCGATGTTTATGGATTCATCCCAGAACAAACAGCAAAACTTTCTATATTGCTGAACAGGTGTCCCATCACCCACCAATATCTGCCTTTTATGTCAGTAACCGGAAAGATGGGTTTTGCCTTAGTGGTAGCATCCTGGCTAAGTCCAAGTTTTATGGAAACTCGTTATCTGCCATATTGGAGGGAGAAGCCCGGTTAACTTTCTTGAACAGAGGTGAAGATTACGTCATGACAATGCCATATGCTCACTGTAAAGGAATTCTTTATGGCACAATGACCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCGATACTTGAATTTAAACTAAAG
Peptide sequence:
MDALELALKCSSLLKRTMVREGKEHDLSISSDSTHVTLYGLLRANNLHSGDNFQLNDSEIERQHFKDQDLYSDKSDKENDPEHDESDNEVLGKSEESDTDTSERQDDSYIDPEPVEPLKETTYMEQSHEELGEAGEASQTETVSEENKSLIWTLLKQVRPGMDLSRVVLPTFILEPRSFLDKLSDYYYHADFLSEAALEENPYFRLKKVVKWYLSGFYKKPKGLKKPYNPILGETFRCLWIHPRTNSKTFYIAEQVSHHPPISAFYVSNRKDGFCLSGSILAKSKFYGNSLSAILEGEARLTFLNRGEDYVMTMPYAHCKGILYGTMTLELGGTVNITCQKTGYSAILEFKLKIRGTLKSWTKLWCVLKPGVLLIYKTQKNGQWVGTVLLNACEIIERPSKKDGFCFKLFHPLEQSIWAVKGPKGEAVGSITQPLPSSYLIIRATSESDGRCWMDALELALKCSSLLKRTMVREGKEHDLSISSDSTHVTLYGLLRANNLHSGDNFQLNDSEIERQHFKDQDLYSDKSDKENDPEHDESDNEVLGKSEESDTDTSERQDDSYIDPEPVEPLKETTYMEQSHEELGEAGEASQTETVSEENKSLIWTLLKQVRPGMDLSRVVLPTFILEPRSFLDKLSDYYYHADFLSEAALEENPYFRLKKVVKWYLSGFYKKPKGLKKPYNPILGETFRCLWIHPRTNSKTFYIAEQVSHHPPISAFYVSNRKDGFCLSGSILAKSKFYGNSLSAILEGEARLTFLNRGEDYVMTMPYAHCKGILYGTMTLELGGTVNITCQKTGYSAILEFKLK
IRESfinder result
IRESfinder score
0.547336803
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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