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mmu_circOsbpl6_496
Basic information of circular RNA
PanCircBase ID:
mmu_circOsbpl6_496
mm39_Chr. Position ID:
chr2|76344260|76354488|+
Gene name:
Osbpl6
Transcript:
ENSMUST00000111929.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,9972
Genomic length:
10228
Exon/intron sizes in nt:
2,40,256
Flanking exon/introns:
chr2:76329766-76344260|chr2:76354488-76370447
Splice length:
496
Other database IDs:
circAtlas: mmu-Osbpl6_0040
Human Ortholog IDs:
Mature spliced Sequence:
GCCTCCAGCAGAAGGTGTGGCCCAGATTAAAGGCGTGTACCACCACAGCTAGATTTGGAACTTGCCCTGTCCCAGGCTGGCCTTGGAATTCAGAGATCTGCTTGCCTCAGTCTCCTGCGATCTTGCCTGAACCTGAGCTTTTTATGGCCACAGTGCCTCAAGATCTCGATCAAAAGGACGTCCCTTGTCAAGATCCAGGTCAGACACCAGAGTCCTCCAGCCTCAAGATCTGGATCACAGGTGGTTTGGACTCGTCAGCAGTGGCCTGAGTTTTCCTAGTCCAGTCTGATCCGAGGGAGATGGACTAAAGCCACAGATTGTCTGCTTCTCTGTCTGTGAATTGCAAGGAGGATTGGGATGGTCTTCCGTGCACATCCCTAGACTAATTGCAGCAATGAGTTCAGACGAGAAGGGCATATCCCCTGCTCATAAAACATCCACTCCGACCCATAGAAGTGCCTCGTCTTCAACATCTTCTCAGAGGGAAAGCAGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCGACCCATAGAAGTGCCTC
59.89
TCTGAATTCCAAGGCCAGCC
60.32
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCGACCCATAGAAGTGCC
59.89
TCTGAATTCCAAGGCCAGCC
60.32
150
PCR template sequence:
GAGTTCAGACGAGAAGGGCATATCCCCTGCTCATAAAACATCCACTCCGACCCATAGAAGTGCCTCGTCTTCAACATCTTCTCAGAGGGAAAGCAGACAGGCCTCCAGCAGAAGGTGTGGCCCAGATTAAAGGCGTGTACCACCACAGCTAGATTTGGAACTTGCCCTGTCCCAGGCTGGCCTTGGAATTCAGAGATCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(2), mmu-miR-31-5p(1), mmu-miR-7a-5p(1), mmu-miR-24-3p(1), mmu-miR-383-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAAAGCAGACAGGCCTCCAGCAGAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCOSBPL6_496_ORF_1
0.9738
-0.052749519
0.061891228
132
ORF sequence:
ATGAGTTCAGACGAGAAGGGCATATCCCCTGCTCATAAAACATCCACTCCGACCCATAGAAGTGCCTCGTCTTCAACATCTTCTCAGAGGGAAAGCAGACAGGCCTCCAGCAGAAGGTGTGGCCCAGATTAA
Peptide sequence:
MSSDEKGISPAHKTSTPTHRSASSSTSSQRESRQASSRRCGPD
IRESfinder result
IRESfinder score
0.704191802
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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