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mmu_circOsbpl6_334
Basic information of circular RNA
PanCircBase ID:
mmu_circOsbpl6_334
mm39_Chr. Position ID:
chr2|76407355|76414892|+
Gene name:
Osbpl6
Transcript:
ENSMUST00000077972.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
16,17,18
Exon/intron offsets:
0,2161,7449
Genomic length:
7537
Exon/intron sizes in nt:
108,138,88
Flanking exon/introns:
chr2:76399387-76407355|chr2:76414892-76415359
Splice length:
334
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GCACTTAACCAGAACGCTGAGCTCCGGAGTCGGCTGAACAGAATACACTCAGAGTCTACCATTTGTGATCACGTTGTCAGTGTAAATATTATCCCTAGCCCCGATGAGCCTGGTGAGCAAATCCATGTCAGCCTCCCTCTGTCACAGCAGGTGGCCAACGAGAGCCGCCTCTCCATGTCAGAATCCGTGTCAGAATTCTTCGATGCCCAAGAGGTGCTCCTGTCTGCAAGCTCATCAGAGAATGAGGCTTCTGATGATGAGTCCTACATCAGCGACGTGAGTGACAACATATCTGAGGACAACACCAGTGTCGCCGACAACATTTCTCGGCAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCCTACATCAGCGACGTGA
59.55
ACACTGACAACGTGATCACA
57.4
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCCTACATCAGCGACGTG
59.55
ACACTGACAACGTGATCACA
57.4
158
PCR template sequence:
TCAGAGAATGAGGCTTCTGATGATGAGTCCTACATCAGCGACGTGAGTGACAACATATCTGAGGACAACACCAGTGTCGCCGACAACATTTCTCGGCAAAGCACTTAACCAGAACGCTGAGCTCCGGAGTCGGCTGAACAGAATACACTCAGAGTCTACCATTTGTGATCACGTTGTCAGTGTAAATATTATCCCTAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-138-5p(1), mmu-miR-370-3p(1), mmu-miR-190b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTTCTCGGCAAAGCACTTAACCAGA
siRNA sets
TTAAGTGCTTTGCCGAGAA, GTTAAGTGCTTTGCCGAGA, TAAGTGCTTTGCCGAGAAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCOSBPL6_334_ORF_3
0.9753
0.430771223
0.384270616
168
ORF sequence:
ATGTCAGAATCCGTGTCAGAATTCTTCGATGCCCAAGAGGTGCTCCTGTCTGCAAGCTCATCAGAGAATGAGGCTTCTGATGATGAGTCCTACATCAGCGACGTGAGTGACAACATATCTGAGGACAACACCAGTGTCGCCGACAACATTTCTCGGCAAAGCACTTAA
Peptide sequence:
MSESVSEFFDAQEVLLSASSSENEASDDESYISDVSDNISEDNTSVADNISRQST
IRESfinder result
IRESfinder score
0.271134204
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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