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mmu_circOsbpl6_300
Basic information of circular RNA
PanCircBase ID:
mmu_circOsbpl6_300
mm39_Chr. Position ID:
chr2|76395127|76398586|+
Gene name:
Osbpl6
Transcript:
ENSMUST00000077972.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
13,14
Exon/intron offsets:
0,3325
Genomic length:
3459
Exon/intron sizes in nt:
1,66,134
Flanking exon/introns:
chr2:76390604-76395127|chr2:76398586-76399363
Splice length:
300
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCCTTTCAGCGCCACCATGTCACCCGTGCGTCTGCACTCCTCCAACCCCAACCTTTGTGCAGACATTGAGTTCCAGACCCCTCCTAGCCACCTCACCGACCCTCTGGAAAGTTCCACGGATTATACCAAGCTACAAGAAGAATTTTGTCTAATTGCACAGAAAGTGCATTCTCTTCTGAAGTCTGCATTTAACAGCATAGCTATAGAGAAGGAGAAGCTGAAGCAGGTGGTCTCGGAGCAAGATCACAACAAAGGCCACAGCACACAGATGGCCAGACTCCGGCAGTCACTGTCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCTGAAGCAGGTGGTCTC
59.96
TCTGCACAAAGGTTGGGGTT
60.03
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGCTGAAGCAGGTGGTCT
59.96
TCTGCACAAAGGTTGGGGTT
60.03
150
PCR template sequence:
AGCTATAGAGAAGGAGAAGCTGAAGCAGGTGGTCTCGGAGCAAGATCACAACAAAGGCCACAGCACACAGATGGCCAGACTCCGGCAGTCACTGTCACAGGTTCCTTTCAGCGCCACCATGTCACCCGTGCGTCTGCACTCCTCCAACCCCAACCTTTGTGCAGACATTGAGTTCCAGACCCCTCCTAGCCACCTCACCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-207(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCACTGTCACAGGTTCCTTTCAGCG
siRNA sets
AAAGGAACCTGTGACAGTG, TGAAAGGAACCTGTGACAG, AAGGAACCTGTGACAGTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCOSBPL6_300_ORF_1
1.0045
0.287061931
0.87915331
582
ORF sequence:
ATGTCACCCGTGCGTCTGCACTCCTCCAACCCCAACCTTTGTGCAGACATTGAGTTCCAGACCCCTCCTAGCCACCTCACCGACCCTCTGGAAAGTTCCACGGATTATACCAAGCTACAAGAAGAATTTTGTCTAATTGCACAGAAAGTGCATTCTCTTCTGAAGTCTGCATTTAACAGCATAGCTATAGAGAAGGAGAAGCTGAAGCAGGTGGTCTCGGAGCAAGATCACAACAAAGGCCACAGCACACAGATGGCCAGACTCCGGCAGTCACTGTCACAGGTTCCTTTCAGCGCCACCATGTCACCCGTGCGTCTGCACTCCTCCAACCCCAACCTTTGTGCAGACATTGAGTTCCAGACCCCTCCTAGCCACCTCACCGACCCTCTGGAAAGTTCCACGGATTATACCAAGCTACAAGAAGAATTTTGTCTAATTGCACAGAAAGTGCATTCTCTTCTGAAGTCTGCATTTAACAGCATAGCTATAGAGAAGGAGAAGCTGAAGCAGGTGGTCTCGGAGCAAGATCACAACAAAGGCCACAGCACACAGATGGCCAGACTCCGGCAGTCACTGTCACAG
Peptide sequence:
MSPVRLHSSNPNLCADIEFQTPPSHLTDPLESSTDYTKLQEEFCLIAQKVHSLLKSAFNSIAIEKEKLKQVVSEQDHNKGHSTQMARLRQSLSQVPFSATMSPVRLHSSNPNLCADIEFQTPPSHLTDPLESSTDYTKLQEEFCLIAQKVHSLLKSAFNSIAIEKEKLKQVVSEQDHNKGHSTQMARLRQSLSQ
IRESfinder result
IRESfinder score
0.456711565
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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