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mmu_circOsbpl6_1075
Basic information of circular RNA
PanCircBase ID:
mmu_circOsbpl6_1075
mm39_Chr. Position ID:
chr2|76415359|76425230|+
Gene name:
Osbpl6
Transcript:
ENSMUST00000077972.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
19,20,21,22,23,24,25,26
Exon/intron offsets:
0,1098,1699,3536,5298,6768,8324,9748
Genomic length:
9871
Exon/intron sizes in nt:
2,54,13,86,47,91,45,14,00,00,000
Flanking exon/introns:
chr2:76414892-76415359|chr2:76425230-76426217
Splice length:
1075
Other database IDs:
circBase: hsa_circ_0118190, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_135098
Human Ortholog IDs:
Mature spliced Sequence:
TCCTGAATGGGGAGCTCACAGGAGGCGCCTTCCGCAATGGCCGTCGCACCTGCCTGCCTGCTCCTTGCCCGGACACCAGTAACATTAACCTGTGGAATATCTTGAGGAACAACATTGGCAAAGACCTGTCGAAGGTCTCCATGCCCGTGGAACTCAACGAGCCCCTCAACACCCTGCAGCACCTCTGTGAGGAAATGGAATACAGTGAGCTCCTGGACAAGGCCTCAGAGACCGACGACCCCTACGAGAGGATGGTTCTCGTCGCTGCCTTTGCAGTCTCAGGGTACTGTTCCACCTACTTCCGAGCAGGAAGCAAGCCATTCAATCCGGTCCTGGGGGAGACCTATGAATGCATAAGAGAAGATAAGGGGTTTCGGTTCTTCTCAGAACAAGTTAGCCATCATCCACCCATTTCTGCCTGTCACTGTGAATCCAAGAATTTTGTGTTTTGGCAAGATATCAGATGGAAAAATAAGTTCTGGGGAAAGTCCATGGAAATCCTGCCTGTCGGAACCCTGAACGTCACGCTTCCAAAATATGGGGATTACTATGTGTGGAATAAAGTCACCACGTGCATACACAATATCCTGAGTGGGAGGAGATGGATCGAGCACTACGGTGAAGTGACCCTTAGAAATACCAAAAGCAGTGTTTGCATTTGCAAACTCACGTTTGTCAAGGTGAATTACTGGAACTCCAACGTGAATGAAGTCCAGGGGGTGGTGATAGACCAGGAGGGGAAGGTGGTGCACCGGCTGTTTGGGAAATGGCACGAAGGACTCTACTGCGGGGTGGCCCCCTCGGCAAAGTGCATCTGGAGACCAGGTTCTCTGCCAACCAACTATGAGCTCTACTATGGCTTTACAAGATTTGCTGTGGAGCTCAATGAGTTGGACCCCGTCCTGAAGGACCTCCTTCCCCCCACAGATGCCCGATTCCGGCCAGATCAAAGGTTTTTGGAAGAAGGGAACCTAGAAGCCGCAGCCGCAGAGAAACAAAGAGTTGAGGAACTCCAAAGATCGCGAAGACGCTACATGGAGGAAAACAACCTTGAACATATACCAAAATTTTTTAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAACTCCAAAGATCGCGA
59.1
AATGTTACTGGTGTCCGGGC
60.32
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCAAAGATCGCGAAGACG
59.08
AATGTTACTGGTGTCCGGGC
60.32
151
PCR template sequence:
AAGCCGCAGCCGCAGAGAAACAAAGAGTTGAGGAACTCCAAAGATCGCGAAGACGCTACATGGAGGAAAACAACCTTGAACATATACCAAAATTTTTTAATCCTGAATGGGGAGCTCACAGGAGGCGCCTTCCGCAATGGCCGTCGCACCTGCCTGCCTGCTCCTTGCCCGGACACCAGTAACATTAACCTGTGGAATAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-135a-5p(1), mmu-miR-101a-3p(1), mmu-miR-125b-5p(1), mmu-miR-125a-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAAATTTTTTAATCCTGAATGGGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCOSBPL6_1075_ORF_1
0.9293
0.214317402
0.990529486
1017
ORF sequence:
ATGCCCGTGGAACTCAACGAGCCCCTCAACACCCTGCAGCACCTCTGTGAGGAAATGGAATACAGTGAGCTCCTGGACAAGGCCTCAGAGACCGACGACCCCTACGAGAGGATGGTTCTCGTCGCTGCCTTTGCAGTCTCAGGGTACTGTTCCACCTACTTCCGAGCAGGAAGCAAGCCATTCAATCCGGTCCTGGGGGAGACCTATGAATGCATAAGAGAAGATAAGGGGTTTCGGTTCTTCTCAGAACAAGTTAGCCATCATCCACCCATTTCTGCCTGTCACTGTGAATCCAAGAATTTTGTGTTTTGGCAAGATATCAGATGGAAAAATAAGTTCTGGGGAAAGTCCATGGAAATCCTGCCTGTCGGAACCCTGAACGTCACGCTTCCAAAATATGGGGATTACTATGTGTGGAATAAAGTCACCACGTGCATACACAATATCCTGAGTGGGAGGAGATGGATCGAGCACTACGGTGAAGTGACCCTTAGAAATACCAAAAGCAGTGTTTGCATTTGCAAACTCACGTTTGTCAAGGTGAATTACTGGAACTCCAACGTGAATGAAGTCCAGGGGGTGGTGATAGACCAGGAGGGGAAGGTGGTGCACCGGCTGTTTGGGAAATGGCACGAAGGACTCTACTGCGGGGTGGCCCCCTCGGCAAAGTGCATCTGGAGACCAGGTTCTCTGCCAACCAACTATGAGCTCTACTATGGCTTTACAAGATTTGCTGTGGAGCTCAATGAGTTGGACCCCGTCCTGAAGGACCTCCTTCCCCCCACAGATGCCCGATTCCGGCCAGATCAAAGGTTTTTGGAAGAAGGGAACCTAGAAGCCGCAGCCGCAGAGAAACAAAGAGTTGAGGAACTCCAAAGATCGCGAAGACGCTACATGGAGGAAAACAACCTTGAACATATACCAAAATTTTTTAATCCTGAATGGGGAGCTCACAGGAGGCGCCTTCCGCAATGGCCGTCGCACCTGCCTGCCTGCTCCTTGCCCGGACACCAGTAA
Peptide sequence:
MPVELNEPLNTLQHLCEEMEYSELLDKASETDDPYERMVLVAAFAVSGYCSTYFRAGSKPFNPVLGETYECIREDKGFRFFSEQVSHHPPISACHCESKNFVFWQDIRWKNKFWGKSMEILPVGTLNVTLPKYGDYYVWNKVTTCIHNILSGRRWIEHYGEVTLRNTKSSVCICKLTFVKVNYWNSNVNEVQGVVIDQEGKVVHRLFGKWHEGLYCGVAPSAKCIWRPGSLPTNYELYYGFTRFAVELNELDPVLKDLLPPTDARFRPDQRFLEEGNLEAAAAEKQRVEELQRSRRRYMEENNLEHIPKFFNPEWGAHRRRLPQWPSHLPACSLPGHQ
IRESfinder result
IRESfinder score
0.418650716
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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