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mmu_circOsbpl2_355
Basic information of circular RNA
PanCircBase ID:
mmu_circOsbpl2_355
mm39_Chr. Position ID:
chr2|179778771|179782973|+
Gene name:
Osbpl2
Transcript:
ENSMUST00000040668.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,1234,4126
Genomic length:
4202
Exon/intron sizes in nt:
134,145,76
Flanking exon/introns:
chr2:179761262-179778771|chr2:179782973-179786624
Splice length:
355
Other database IDs:
circBase: mmu_circ_0009656, CIRCpedia: MMU_CIRCpedia_12272, circAtlas: mmu-Osbpl2_0001
circBase: hsa_circ_0115550, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_34912
Human Ortholog IDs:
Mature spliced Sequence:
ATTTCCAGGATCCACACAACTTCAACATAAATATAGATCAGATATGATCCAGTAGGAGGACCAAACCCCAGAACAGTGACAGTCTTGTCACTGAAGGATGAACGGGGAGGAAGAATTCTTTGATGCCGTCACAGGCTTTGATTCTGATAATTCTTCTATAGGGGAATTTTCAGAGGCAAATAAAATTAGTGGGATGATTGACTTGGATACCAGCAAAAGTACTAGGAGTGGGAAAAATGGGGAGAAACCACAACAAGAGAATGGAATTCAAAAGCACAGGACAGCCCTTCCTGCTCCCATGTTCACCAGAAGCGACTTCAGTGTGTGGAGTATCCTGAAGAAATGCATTGGCTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTGCTCCCATGTTCACC
59.96
ACTGTTCTGGGGTTTGGTCC
59.81
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTGCTCCCATGTTCACC
59.96
GTTCTGGGGTTTGGTCCTCC
60.25
142
PCR template sequence:
AGAGAATGGAATTCAAAAGCACAGGACAGCCCTTCCTGCTCCCATGTTCACCAGAAGCGACTTCAGTGTGTGGAGTATCCTGAAGAAATGCATTGGCTTGATTTCCAGGATCCACACAACTTCAACATAAATATAGATCAGATATGATCCAGTAGGAGGACCAAACCCCAGAACAGTGACAGTCTTGTCACTGAAGGATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
ATGCATTGGCTTGATTTCCAGGATCC
siRNA sets
GGATCCTGGAAATCAAGCC, GATCCTGGAAATCAAGCCA, CTGGAAATCAAGCCAATGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCOSBPL2_355_ORF_1
0.9345
0.077097563
0.244464352
288
ORF sequence:
ATGAACGGGGAGGAAGAATTCTTTGATGCCGTCACAGGCTTTGATTCTGATAATTCTTCTATAGGGGAATTTTCAGAGGCAAATAAAATTAGTGGGATGATTGACTTGGATACCAGCAAAAGTACTAGGAGTGGGAAAAATGGGGAGAAACCACAACAAGAGAATGGAATTCAAAAGCACAGGACAGCCCTTCCTGCTCCCATGTTCACCAGAAGCGACTTCAGTGTGTGGAGTATCCTGAAGAAATGCATTGGCTTGATTTCCAGGATCCACACAACTTCAACATAA
Peptide sequence:
MNGEEEFFDAVTGFDSDNSSIGEFSEANKISGMIDLDTSKSTRSGKNGEKPQQENGIQKHRTALPAPMFTRSDFSVWSILKKCIGLISRIHTTST
IRESfinder result
IRESfinder score
0.737767875
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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